Effects of choline on the phenotype of the cultured bovine preimplantation embryo

Autores
Estrada Cortés, E.; Negrón Pérez, Verónica; Tribulo, Paula; Zenobi, Marcos Gonzalo; Staples, Charles; Hansen, P. J.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Choline is a precursor of acetylcholine, phosphatidylcholine, and the methyl-donor betaine. Reports indicate that supplementation with rumen-protected choline improves postpartum reproductive function of dairy cows. The objective was to determine whether addition of choline to culture medium of in vitro-produced embryos alters the phenotype of the resultant blastocysts. Treatments were choline chloride (ChCl; 0.004, 1.3, 1.8, and 6.37 mM) and phosphatidylcholine (1.3 mM). Treatment with 0.004 mM ChCl improved development to the blastocyst stage, increased blastocyst cell number, and increased the percentage of blastocysts that were hatching or hatched. Development was not affected by higher concentrations of ChCl but was reduced by 1.3 mM phosphatidylcholine. Treatment of embryos with 1.3 mM ChCl (but not other concentrations) increased expression in blastocysts of 11 of 165 genes examined (AMOT, NANOG, HDAC8, HNF4A, STAT1, MBNL3, SOX2, STAT3, KDM2B, SAV1, and GPAM) and decreased expression of one gene (ASS1). Treatment with 1.3 mM ChCl decreased global DNA methylation at d 3.5 of development and increased DNA methylation at d 7.5 in blastocysts. Treatment with 1.8 mM ChCl also increased methylation in blastocysts. In conclusion, addition of choline to the culture medium alters the phenotype of preimplantation bovine embryos produced in vitro. Choline chloride can act in a concentrationdependent manner to alter development, expression of specific genes, and DNA methylation.
Fil: Estrada Cortés, E.. University of Florida; Estados Unidos. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias; México
Fil: Negrón Pérez, Verónica. Universidad de Puerto Rico; Puerto Rico
Fil: Tribulo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto de Reproducción Animal Córdoba; Argentina
Fil: Zenobi, Marcos Gonzalo. Instituto de Reproducción Animal Córdoba; Argentina
Fil: Staples, Charles. University of Florida; Estados Unidos
Fil: Hansen, P. J.. University of Florida; Estados Unidos
Materia
Ccholine
Embryo
Blastocyst
Gene expression
DNA methylation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/267582
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/267582
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of choline on the phenotype of the cultured bovine preimplantation embryoEstrada Cortés, E.Negrón Pérez, VerónicaTribulo, PaulaZenobi, Marcos GonzaloStaples, CharlesHansen, P. J.CcholineEmbryoBlastocystGene expressionDNA methylationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Choline is a precursor of acetylcholine, phosphatidylcholine, and the methyl-donor betaine. Reports indicate that supplementation with rumen-protected choline improves postpartum reproductive function of dairy cows. The objective was to determine whether addition of choline to culture medium of in vitro-produced embryos alters the phenotype of the resultant blastocysts. Treatments were choline chloride (ChCl; 0.004, 1.3, 1.8, and 6.37 mM) and phosphatidylcholine (1.3 mM). Treatment with 0.004 mM ChCl improved development to the blastocyst stage, increased blastocyst cell number, and increased the percentage of blastocysts that were hatching or hatched. Development was not affected by higher concentrations of ChCl but was reduced by 1.3 mM phosphatidylcholine. Treatment of embryos with 1.3 mM ChCl (but not other concentrations) increased expression in blastocysts of 11 of 165 genes examined (AMOT, NANOG, HDAC8, HNF4A, STAT1, MBNL3, SOX2, STAT3, KDM2B, SAV1, and GPAM) and decreased expression of one gene (ASS1). Treatment with 1.3 mM ChCl decreased global DNA methylation at d 3.5 of development and increased DNA methylation at d 7.5 in blastocysts. Treatment with 1.8 mM ChCl also increased methylation in blastocysts. In conclusion, addition of choline to the culture medium alters the phenotype of preimplantation bovine embryos produced in vitro. Choline chloride can act in a concentrationdependent manner to alter development, expression of specific genes, and DNA methylation.Fil: Estrada Cortés, E.. University of Florida; Estados Unidos. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias; MéxicoFil: Negrón Pérez, Verónica. Universidad de Puerto Rico; Puerto RicoFil: Tribulo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto de Reproducción Animal Córdoba; ArgentinaFil: Zenobi, Marcos Gonzalo. Instituto de Reproducción Animal Córdoba; ArgentinaFil: Staples, Charles. University of Florida; Estados UnidosFil: Hansen, P. J.. University of Florida; Estados UnidosAmerican Dairy Science Association2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/267582Estrada Cortés, E.; Negrón Pérez, Verónica; Tribulo, Paula; Zenobi, Marcos Gonzalo; Staples, Charles; et al.; Effects of choline on the phenotype of the cultured bovine preimplantation embryo; American Dairy Science Association; Journal of Dairy Science; 103; 11; 11-2020; 10784-107960022-0302CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022030220306640info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.2020-18598info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:54Zoai:ri.conicet.gov.ar:11336/267582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:54.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of choline on the phenotype of the cultured bovine preimplantation embryo
title Effects of choline on the phenotype of the cultured bovine preimplantation embryo
spellingShingle Effects of choline on the phenotype of the cultured bovine preimplantation embryo
Estrada Cortés, E.
Ccholine
Embryo
Blastocyst
Gene expression
DNA methylation
title_short Effects of choline on the phenotype of the cultured bovine preimplantation embryo
title_full Effects of choline on the phenotype of the cultured bovine preimplantation embryo
title_fullStr Effects of choline on the phenotype of the cultured bovine preimplantation embryo
title_full_unstemmed Effects of choline on the phenotype of the cultured bovine preimplantation embryo
title_sort Effects of choline on the phenotype of the cultured bovine preimplantation embryo
dc.creator.none.fl_str_mv Estrada Cortés, E.
Negrón Pérez, Verónica
Tribulo, Paula
Zenobi, Marcos Gonzalo
Staples, Charles
Hansen, P. J.
author Estrada Cortés, E.
author_facet Estrada Cortés, E.
Negrón Pérez, Verónica
Tribulo, Paula
Zenobi, Marcos Gonzalo
Staples, Charles
Hansen, P. J.
author_role author
author2 Negrón Pérez, Verónica
Tribulo, Paula
Zenobi, Marcos Gonzalo
Staples, Charles
Hansen, P. J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ccholine
Embryo
Blastocyst
Gene expression
DNA methylation
topic Ccholine
Embryo
Blastocyst
Gene expression
DNA methylation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Choline is a precursor of acetylcholine, phosphatidylcholine, and the methyl-donor betaine. Reports indicate that supplementation with rumen-protected choline improves postpartum reproductive function of dairy cows. The objective was to determine whether addition of choline to culture medium of in vitro-produced embryos alters the phenotype of the resultant blastocysts. Treatments were choline chloride (ChCl; 0.004, 1.3, 1.8, and 6.37 mM) and phosphatidylcholine (1.3 mM). Treatment with 0.004 mM ChCl improved development to the blastocyst stage, increased blastocyst cell number, and increased the percentage of blastocysts that were hatching or hatched. Development was not affected by higher concentrations of ChCl but was reduced by 1.3 mM phosphatidylcholine. Treatment of embryos with 1.3 mM ChCl (but not other concentrations) increased expression in blastocysts of 11 of 165 genes examined (AMOT, NANOG, HDAC8, HNF4A, STAT1, MBNL3, SOX2, STAT3, KDM2B, SAV1, and GPAM) and decreased expression of one gene (ASS1). Treatment with 1.3 mM ChCl decreased global DNA methylation at d 3.5 of development and increased DNA methylation at d 7.5 in blastocysts. Treatment with 1.8 mM ChCl also increased methylation in blastocysts. In conclusion, addition of choline to the culture medium alters the phenotype of preimplantation bovine embryos produced in vitro. Choline chloride can act in a concentrationdependent manner to alter development, expression of specific genes, and DNA methylation.
Fil: Estrada Cortés, E.. University of Florida; Estados Unidos. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias; México
Fil: Negrón Pérez, Verónica. Universidad de Puerto Rico; Puerto Rico
Fil: Tribulo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina. Instituto de Reproducción Animal Córdoba; Argentina
Fil: Zenobi, Marcos Gonzalo. Instituto de Reproducción Animal Córdoba; Argentina
Fil: Staples, Charles. University of Florida; Estados Unidos
Fil: Hansen, P. J.. University of Florida; Estados Unidos
description Choline is a precursor of acetylcholine, phosphatidylcholine, and the methyl-donor betaine. Reports indicate that supplementation with rumen-protected choline improves postpartum reproductive function of dairy cows. The objective was to determine whether addition of choline to culture medium of in vitro-produced embryos alters the phenotype of the resultant blastocysts. Treatments were choline chloride (ChCl; 0.004, 1.3, 1.8, and 6.37 mM) and phosphatidylcholine (1.3 mM). Treatment with 0.004 mM ChCl improved development to the blastocyst stage, increased blastocyst cell number, and increased the percentage of blastocysts that were hatching or hatched. Development was not affected by higher concentrations of ChCl but was reduced by 1.3 mM phosphatidylcholine. Treatment of embryos with 1.3 mM ChCl (but not other concentrations) increased expression in blastocysts of 11 of 165 genes examined (AMOT, NANOG, HDAC8, HNF4A, STAT1, MBNL3, SOX2, STAT3, KDM2B, SAV1, and GPAM) and decreased expression of one gene (ASS1). Treatment with 1.3 mM ChCl decreased global DNA methylation at d 3.5 of development and increased DNA methylation at d 7.5 in blastocysts. Treatment with 1.8 mM ChCl also increased methylation in blastocysts. In conclusion, addition of choline to the culture medium alters the phenotype of preimplantation bovine embryos produced in vitro. Choline chloride can act in a concentrationdependent manner to alter development, expression of specific genes, and DNA methylation.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/267582
Estrada Cortés, E.; Negrón Pérez, Verónica; Tribulo, Paula; Zenobi, Marcos Gonzalo; Staples, Charles; et al.; Effects of choline on the phenotype of the cultured bovine preimplantation embryo; American Dairy Science Association; Journal of Dairy Science; 103; 11; 11-2020; 10784-10796
0022-0302
CONICET Digital
CONICET
url http://hdl.handle.net/11336/267582
identifier_str_mv Estrada Cortés, E.; Negrón Pérez, Verónica; Tribulo, Paula; Zenobi, Marcos Gonzalo; Staples, Charles; et al.; Effects of choline on the phenotype of the cultured bovine preimplantation embryo; American Dairy Science Association; Journal of Dairy Science; 103; 11; 11-2020; 10784-10796
0022-0302
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022030220306640
info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.2020-18598
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Dairy Science Association
publisher.none.fl_str_mv American Dairy Science Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268824323227648
score 13.13397

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