Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
- Autores
- Cebral, E.; Carrasco, I.; Vantman, D.; Smith, R.
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. - Fuente
- Biocell 2007;31(1):51-59
- Materia
-
Mouse
Preimplantation embryogenesis
Reactive oxygen species
Toxicity
hydrogen peroxide
reactive oxygen metabolite
animal experiment
animal model
article
cell cycle arrest
controlled study
culture medium
embryo
embryo development
embryotoxicity
female
male
mouse
nonhuman
oxidative stress
preimplantation embryo
Animals
Blastocyst
Cleavage Stage, Ovum
Embryo Transfer
Embryonic Development
Female
Hydrogen Peroxide
Male
Mice
Oxidative Stress
Reactive Oxygen Species - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/2.5/ar
- Repositorio
- Institución
- Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
- OAI Identificador
- paperaa:paper_03279545_v31_n1_p51_Cebral
Ver los metadatos del registro completo
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Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen speciesCebral, E.Carrasco, I.Vantman, D.Smith, R.MousePreimplantation embryogenesisReactive oxygen speciesToxicityhydrogen peroxidereactive oxygen metaboliteanimal experimentanimal modelarticlecell cycle arrestcontrolled studyculture mediumembryoembryo developmentembryotoxicityfemalemalemousenonhumanoxidative stresspreimplantation embryoAnimalsBlastocystCleavage Stage, OvumEmbryo TransferEmbryonic DevelopmentFemaleHydrogen PeroxideMaleMiceOxidative StressReactive Oxygen SpeciesExposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_CebralBiocell 2007;31(1):51-59reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_03279545_v31_n1_p51_CebralInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.154Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse |
dc.title.none.fl_str_mv |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
title |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
spellingShingle |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species Cebral, E. Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species |
title_short |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
title_full |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
title_fullStr |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
title_full_unstemmed |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
title_sort |
Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species |
dc.creator.none.fl_str_mv |
Cebral, E. Carrasco, I. Vantman, D. Smith, R. |
author |
Cebral, E. |
author_facet |
Cebral, E. Carrasco, I. Vantman, D. Smith, R. |
author_role |
author |
author2 |
Carrasco, I. Vantman, D. Smith, R. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species |
topic |
Mouse Preimplantation embryogenesis Reactive oxygen species Toxicity hydrogen peroxide reactive oxygen metabolite animal experiment animal model article cell cycle arrest controlled study culture medium embryo embryo development embryotoxicity female male mouse nonhuman oxidative stress preimplantation embryo Animals Blastocyst Cleavage Stage, Ovum Embryo Transfer Embryonic Development Female Hydrogen Peroxide Male Mice Oxidative Stress Reactive Oxygen Species |
dc.description.none.fl_txt_mv |
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
description |
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral |
url |
http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/ar |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Biocell 2007;31(1):51-59 reponame:Biblioteca Digital (UBA-FCEN) instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN |
reponame_str |
Biblioteca Digital (UBA-FCEN) |
collection |
Biblioteca Digital (UBA-FCEN) |
instname_str |
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
instacron_str |
UBA-FCEN |
institution |
UBA-FCEN |
repository.name.fl_str_mv |
Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
repository.mail.fl_str_mv |
ana@bl.fcen.uba.ar |
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