Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species

Autores
Cebral, E.; Carrasco, I.; Vantman, D.; Smith, R.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p<0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p<0.05 and p<0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
Biocell 2007;31(1):51-59
Materia
Mouse
Preimplantation embryogenesis
Reactive oxygen species
Toxicity
hydrogen peroxide
reactive oxygen metabolite
animal experiment
animal model
article
cell cycle arrest
controlled study
culture medium
embryo
embryo development
embryotoxicity
female
male
mouse
nonhuman
oxidative stress
preimplantation embryo
Animals
Blastocyst
Cleavage Stage, Ovum
Embryo Transfer
Embryonic Development
Female
Hydrogen Peroxide
Male
Mice
Oxidative Stress
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_03279545_v31_n1_p51_Cebral

id BDUBAFCEN_9a36d060989a7c18a0893f9f7904415c
oai_identifier_str paperaa:paper_03279545_v31_n1_p51_Cebral
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen speciesCebral, E.Carrasco, I.Vantman, D.Smith, R.MousePreimplantation embryogenesisReactive oxygen speciesToxicityhydrogen peroxidereactive oxygen metaboliteanimal experimentanimal modelarticlecell cycle arrestcontrolled studyculture mediumembryoembryo developmentembryotoxicityfemalemalemousenonhumanoxidative stresspreimplantation embryoAnimalsBlastocystCleavage Stage, OvumEmbryo TransferEmbryonic DevelopmentFemaleHydrogen PeroxideMaleMiceOxidative StressReactive Oxygen SpeciesExposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p&lt;0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p&lt;0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p&lt;0.05 and p&lt;0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_CebralBiocell 2007;31(1):51-59reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:05Zpaperaa:paper_03279545_v31_n1_p51_CebralInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:07.154Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
spellingShingle Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
Cebral, E.
Mouse
Preimplantation embryogenesis
Reactive oxygen species
Toxicity
hydrogen peroxide
reactive oxygen metabolite
animal experiment
animal model
article
cell cycle arrest
controlled study
culture medium
embryo
embryo development
embryotoxicity
female
male
mouse
nonhuman
oxidative stress
preimplantation embryo
Animals
Blastocyst
Cleavage Stage, Ovum
Embryo Transfer
Embryonic Development
Female
Hydrogen Peroxide
Male
Mice
Oxidative Stress
Reactive Oxygen Species
title_short Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_full Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_fullStr Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_full_unstemmed Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
title_sort Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species
dc.creator.none.fl_str_mv Cebral, E.
Carrasco, I.
Vantman, D.
Smith, R.
author Cebral, E.
author_facet Cebral, E.
Carrasco, I.
Vantman, D.
Smith, R.
author_role author
author2 Carrasco, I.
Vantman, D.
Smith, R.
author2_role author
author
author
dc.subject.none.fl_str_mv Mouse
Preimplantation embryogenesis
Reactive oxygen species
Toxicity
hydrogen peroxide
reactive oxygen metabolite
animal experiment
animal model
article
cell cycle arrest
controlled study
culture medium
embryo
embryo development
embryotoxicity
female
male
mouse
nonhuman
oxidative stress
preimplantation embryo
Animals
Blastocyst
Cleavage Stage, Ovum
Embryo Transfer
Embryonic Development
Female
Hydrogen Peroxide
Male
Mice
Oxidative Stress
Reactive Oxygen Species
topic Mouse
Preimplantation embryogenesis
Reactive oxygen species
Toxicity
hydrogen peroxide
reactive oxygen metabolite
animal experiment
animal model
article
cell cycle arrest
controlled study
culture medium
embryo
embryo development
embryotoxicity
female
male
mouse
nonhuman
oxidative stress
preimplantation embryo
Animals
Blastocyst
Cleavage Stage, Ovum
Embryo Transfer
Embryonic Development
Female
Hydrogen Peroxide
Male
Mice
Oxidative Stress
Reactive Oxygen Species
dc.description.none.fl_txt_mv Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p&lt;0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p&lt;0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p&lt;0.05 and p&lt;0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H2O2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p&lt;0.001). Twenty-five μM H2O2 produced inhibition of blastocyst formation (p&lt;0.001) and 10 μM H2O2 significantly decreased the percentages of expanded and hatched blastocysts, which resulted morphologically altered (p&lt;0.05 and p&lt;0.01, respectively). The higher H2O2 concentrations were able to elicit necrotic morphology in the 2-cell arrested embryos, while 10 μM H2O 2 induced moderate damage with the arrested embryos partially fragmented. In conclusion, important causes for defective preimplantation development and for early embryo losses may be due to oxidative stress because early mouse embryos exposed to ROS for short times arrested at the first cellular cycle (2-cell) and/or impaired embryo differentiation and morphogenesis, being these effects ROS-concentration-dependent.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral
url http://hdl.handle.net/20.500.12110/paper_03279545_v31_n1_p51_Cebral
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biocell 2007;31(1):51-59
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
_version_ 1844618739377504256
score 13.070432