Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts

Autores
Barrera, Antonio Daniel; Garcia, Elina Vanesa; Hamdi, Meriem; Sánchez Calabuig, María Jesús; López Cardona, Ángela Patricia; Fonseca Balvís, Noelia; Rizos, Dimitrios; Gutiérrez Adan, Alfonso
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1.25% OF added at the one- to 16-cell stage (OF1-16), one- to 8-cell stage (OF1-8) or 8- to 16-cell stage (OF8-16), and then were cultured until Day 8 in medium with 3 mg/mL BSA. Genomic regions in four developmentally important genes (MTERF2, ABCA7, OLFM1, GMDS) and within LINE-1 retrotransposons were selected for methylation analysis by bisulfite sequencing on Day 7-8 blastocysts. Blastocysts derived from OF1-16 group showed lower CpG methylation levels in MTERF2 and ABCA7 compared with the BSA group. However, CpG sites within MTERF2, ABCA7 and OLFM1 showed higher methylation levels in groups OF1-8 and OF8-16 than in OF1-16. For LINE-1 elements, higher CpG methylation levels were observed in blastocysts from the OF1-16 group than in the other experimental groups. In correlation with the methylation changes observed, mRNA expression level of MTERF2 was increased, while LINE-1 showed a decreased expression in blastocysts from OF1-16 group. Our results suggest that embryos show transient sensitivity to OF at early stages, which is reflected by specific methylation changes at the blastocyst stage.
Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: López Cardona, Ángela Patricia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Fonseca Balvís, Noelia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Materia
BOVINE
BLASTOCYST
DNA METHYLATION
OVIDUCT
OVIDUCTAL FLUID
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53858
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/53858
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocystsBarrera, Antonio DanielGarcia, Elina VanesaHamdi, MeriemSánchez Calabuig, María JesúsLópez Cardona, Ángela PatriciaFonseca Balvís, NoeliaRizos, DimitriosGutiérrez Adan, AlfonsoBOVINEBLASTOCYSTDNA METHYLATIONOVIDUCTOVIDUCTAL FLUIDhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1.25% OF added at the one- to 16-cell stage (OF1-16), one- to 8-cell stage (OF1-8) or 8- to 16-cell stage (OF8-16), and then were cultured until Day 8 in medium with 3 mg/mL BSA. Genomic regions in four developmentally important genes (MTERF2, ABCA7, OLFM1, GMDS) and within LINE-1 retrotransposons were selected for methylation analysis by bisulfite sequencing on Day 7-8 blastocysts. Blastocysts derived from OF1-16 group showed lower CpG methylation levels in MTERF2 and ABCA7 compared with the BSA group. However, CpG sites within MTERF2, ABCA7 and OLFM1 showed higher methylation levels in groups OF1-8 and OF8-16 than in OF1-16. For LINE-1 elements, higher CpG methylation levels were observed in blastocysts from the OF1-16 group than in the other experimental groups. In correlation with the methylation changes observed, mRNA expression level of MTERF2 was increased, while LINE-1 showed a decreased expression in blastocysts from OF1-16 group. Our results suggest that embryos show transient sensitivity to OF at early stages, which is reflected by specific methylation changes at the blastocyst stage.Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaFil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaFil: López Cardona, Ángela Patricia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaFil: Fonseca Balvís, Noelia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaFil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaFil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; EspañaBioScientifica2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53858Barrera, Antonio Daniel; Garcia, Elina Vanesa; Hamdi, Meriem; Sánchez Calabuig, María Jesús; López Cardona, Ángela Patricia; et al.; Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts; BioScientifica; Reproduction; 154; 1; 4-2017; 1-121470-1626CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/154/1/1info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-16-0651info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:58Zoai:ri.conicet.gov.ar:11336/53858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:59.265CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
title Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
spellingShingle Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
Barrera, Antonio Daniel
BOVINE
BLASTOCYST
DNA METHYLATION
OVIDUCT
OVIDUCTAL FLUID
title_short Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
title_full Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
title_fullStr Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
title_full_unstemmed Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
title_sort Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts
dc.creator.none.fl_str_mv Barrera, Antonio Daniel
Garcia, Elina Vanesa
Hamdi, Meriem
Sánchez Calabuig, María Jesús
López Cardona, Ángela Patricia
Fonseca Balvís, Noelia
Rizos, Dimitrios
Gutiérrez Adan, Alfonso
author Barrera, Antonio Daniel
author_facet Barrera, Antonio Daniel
Garcia, Elina Vanesa
Hamdi, Meriem
Sánchez Calabuig, María Jesús
López Cardona, Ángela Patricia
Fonseca Balvís, Noelia
Rizos, Dimitrios
Gutiérrez Adan, Alfonso
author_role author
author2 Garcia, Elina Vanesa
Hamdi, Meriem
Sánchez Calabuig, María Jesús
López Cardona, Ángela Patricia
Fonseca Balvís, Noelia
Rizos, Dimitrios
Gutiérrez Adan, Alfonso
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE
BLASTOCYST
DNA METHYLATION
OVIDUCT
OVIDUCTAL FLUID
topic BOVINE
BLASTOCYST
DNA METHYLATION
OVIDUCT
OVIDUCTAL FLUID
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1.25% OF added at the one- to 16-cell stage (OF1-16), one- to 8-cell stage (OF1-8) or 8- to 16-cell stage (OF8-16), and then were cultured until Day 8 in medium with 3 mg/mL BSA. Genomic regions in four developmentally important genes (MTERF2, ABCA7, OLFM1, GMDS) and within LINE-1 retrotransposons were selected for methylation analysis by bisulfite sequencing on Day 7-8 blastocysts. Blastocysts derived from OF1-16 group showed lower CpG methylation levels in MTERF2 and ABCA7 compared with the BSA group. However, CpG sites within MTERF2, ABCA7 and OLFM1 showed higher methylation levels in groups OF1-8 and OF8-16 than in OF1-16. For LINE-1 elements, higher CpG methylation levels were observed in blastocysts from the OF1-16 group than in the other experimental groups. In correlation with the methylation changes observed, mRNA expression level of MTERF2 was increased, while LINE-1 showed a decreased expression in blastocysts from OF1-16 group. Our results suggest that embryos show transient sensitivity to OF at early stages, which is reflected by specific methylation changes at the blastocyst stage.
Fil: Barrera, Antonio Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Garcia, Elina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Hamdi, Meriem. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Sánchez Calabuig, María Jesús. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: López Cardona, Ángela Patricia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Fonseca Balvís, Noelia. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Rizos, Dimitrios. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
Fil: Gutiérrez Adan, Alfonso. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Departamento de Reproducción Animal; España
description During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu could affect the DNA methylation marks of the developing embryo. The aim of this study was to examine if culture of bovine embryos with oviductal fluid (OF) can induce DNA methylation changes at specific genomic regions in the resulting blastocysts. In vitro produced zygotes were cultured in medium with 3 mg/mL bovine serum albumin (BSA) or 1.25% OF added at the one- to 16-cell stage (OF1-16), one- to 8-cell stage (OF1-8) or 8- to 16-cell stage (OF8-16), and then were cultured until Day 8 in medium with 3 mg/mL BSA. Genomic regions in four developmentally important genes (MTERF2, ABCA7, OLFM1, GMDS) and within LINE-1 retrotransposons were selected for methylation analysis by bisulfite sequencing on Day 7-8 blastocysts. Blastocysts derived from OF1-16 group showed lower CpG methylation levels in MTERF2 and ABCA7 compared with the BSA group. However, CpG sites within MTERF2, ABCA7 and OLFM1 showed higher methylation levels in groups OF1-8 and OF8-16 than in OF1-16. For LINE-1 elements, higher CpG methylation levels were observed in blastocysts from the OF1-16 group than in the other experimental groups. In correlation with the methylation changes observed, mRNA expression level of MTERF2 was increased, while LINE-1 showed a decreased expression in blastocysts from OF1-16 group. Our results suggest that embryos show transient sensitivity to OF at early stages, which is reflected by specific methylation changes at the blastocyst stage.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53858
Barrera, Antonio Daniel; Garcia, Elina Vanesa; Hamdi, Meriem; Sánchez Calabuig, María Jesús; López Cardona, Ángela Patricia; et al.; Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts; BioScientifica; Reproduction; 154; 1; 4-2017; 1-12
1470-1626
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53858
identifier_str_mv Barrera, Antonio Daniel; Garcia, Elina Vanesa; Hamdi, Meriem; Sánchez Calabuig, María Jesús; López Cardona, Ángela Patricia; et al.; Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts; BioScientifica; Reproduction; 154; 1; 4-2017; 1-12
1470-1626
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.reproduction-online.org/content/154/1/1
info:eu-repo/semantics/altIdentifier/doi/10.1530/REP-16-0651
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioScientifica
publisher.none.fl_str_mv BioScientifica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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