Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time

Autores
Andreozzi, Patrizia; Simó, Cristina; Moretti, Paolo; Martinez Porcel, Joaquin; Lüdtke, Tanja Ursula; Ramirez, Maria de Los Angeles; Tamberi, Lorenza; Marradi, Marco; Amenitsch, Heinz; Llop, Jordi; Ortore, Maria Grazia; Moya, Sergio Enrique
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An approach for reducing toxicity and enhancing therapeutic potential of supramolecular polyamine phosphate nanoparticles (PANs) through PEGylation of polyamines before their assembly into nanoparticles is presented here. It is shown that the number of polyethylene glycol (PEG) chains for polyamine largely influence physico-chemical properties of PANs and their biological endpoints. Poly(allylamine hydrochloride) (PAH) are functionalized through carbodiimide chemistry with three ratios of PEG molecules per PAH chain: 0.1, 1, and 10. PEGylated PAH is then assembled into PANs by exposing the polymer to phosphate buffer solution. PANs decrease size and surface charge with increasing PEG ratios as evidenced by dynamic light scattering and zeta potential measurements, with the ten PEG/PAH ratio PANs having practically zero charge. Small angle X-ray scattering (SAXS) proves that PEG chains form a shell around a polyamine core, which is responsible for the screening of positive charges. MTT experiments show that the screening of amine groups decreases nanoparticle toxicity, with the lowest toxicity for the 10 PEG/PAH ratio. Fluorescence correlation spectroscopy (FCS) proves less interaction with proteins for PEGylated PANs. Positron emission tomography (PET) imaging of 18F labelled PANs shows longer circulation time in healthy mice for PEGylated PANs than non-PEGylated ones.
Fil: Andreozzi, Patrizia. Basque Research and Technology Alliance; España. Università degli Studi di Firenze; Italia
Fil: Simó, Cristina. Basque Research and Technology Alliance; España
Fil: Moretti, Paolo. Università Politecnica delle Marche; Italia
Fil: Martinez Porcel, Joaquin. Basque Research and Technology Alliance; España
Fil: Lüdtke, Tanja Ursula. Basque Research and Technology Alliance; España
Fil: Ramirez, Maria de Los Angeles. Basque Research and Technology Alliance; España. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tamberi, Lorenza. Basque Research and Technology Alliance; España
Fil: Marradi, Marco. Università degli Studi di Firenze; Italia
Fil: Amenitsch, Heinz. Graz University Of Technology.; Austria
Fil: Llop, Jordi. Basque Research and Technology Alliance; España. Centro de Investigación Biomédica En Red de Enfermedades Respiratorias; España
Fil: Ortore, Maria Grazia. Università Politecnica Delle Marche; Italia
Fil: Moya, Sergio Enrique. Basque Research and Technology Alliance; España
Materia
BIOLOGICAL FATE
POLYAMINE PHOSPHATE NANOPARTICLES
POLYETHYLENE GLYCOL
SELF ASSEMBLY
SMALL ANGLE X-RAY SCATTERING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/166058
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/166058
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation TimeAndreozzi, PatriziaSimó, CristinaMoretti, PaoloMartinez Porcel, JoaquinLüdtke, Tanja UrsulaRamirez, Maria de Los AngelesTamberi, LorenzaMarradi, MarcoAmenitsch, HeinzLlop, JordiOrtore, Maria GraziaMoya, Sergio EnriqueBIOLOGICAL FATEPOLYAMINE PHOSPHATE NANOPARTICLESPOLYETHYLENE GLYCOLSELF ASSEMBLYSMALL ANGLE X-RAY SCATTERINGhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An approach for reducing toxicity and enhancing therapeutic potential of supramolecular polyamine phosphate nanoparticles (PANs) through PEGylation of polyamines before their assembly into nanoparticles is presented here. It is shown that the number of polyethylene glycol (PEG) chains for polyamine largely influence physico-chemical properties of PANs and their biological endpoints. Poly(allylamine hydrochloride) (PAH) are functionalized through carbodiimide chemistry with three ratios of PEG molecules per PAH chain: 0.1, 1, and 10. PEGylated PAH is then assembled into PANs by exposing the polymer to phosphate buffer solution. PANs decrease size and surface charge with increasing PEG ratios as evidenced by dynamic light scattering and zeta potential measurements, with the ten PEG/PAH ratio PANs having practically zero charge. Small angle X-ray scattering (SAXS) proves that PEG chains form a shell around a polyamine core, which is responsible for the screening of positive charges. MTT experiments show that the screening of amine groups decreases nanoparticle toxicity, with the lowest toxicity for the 10 PEG/PAH ratio. Fluorescence correlation spectroscopy (FCS) proves less interaction with proteins for PEGylated PANs. Positron emission tomography (PET) imaging of 18F labelled PANs shows longer circulation time in healthy mice for PEGylated PANs than non-PEGylated ones.Fil: Andreozzi, Patrizia. Basque Research and Technology Alliance; España. Università degli Studi di Firenze; ItaliaFil: Simó, Cristina. Basque Research and Technology Alliance; EspañaFil: Moretti, Paolo. Università Politecnica delle Marche; ItaliaFil: Martinez Porcel, Joaquin. Basque Research and Technology Alliance; EspañaFil: Lüdtke, Tanja Ursula. Basque Research and Technology Alliance; EspañaFil: Ramirez, Maria de Los Angeles. Basque Research and Technology Alliance; España. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tamberi, Lorenza. Basque Research and Technology Alliance; EspañaFil: Marradi, Marco. Università degli Studi di Firenze; ItaliaFil: Amenitsch, Heinz. Graz University Of Technology.; AustriaFil: Llop, Jordi. Basque Research and Technology Alliance; España. Centro de Investigación Biomédica En Red de Enfermedades Respiratorias; EspañaFil: Ortore, Maria Grazia. Università Politecnica Delle Marche; ItaliaFil: Moya, Sergio Enrique. Basque Research and Technology Alliance; EspañaWiley VCH Verlag2021-09-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/166058Andreozzi, Patrizia; Simó, Cristina; Moretti, Paolo; Martinez Porcel, Joaquin; Lüdtke, Tanja Ursula; et al.; Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time; Wiley VCH Verlag; Small; 17; 35; 2-9-2021; 1-101613-68101613-6829CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/smll.202102211info:eu-repo/semantics/altIdentifier/doi/10.1002/smll.202102211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:19:47Zoai:ri.conicet.gov.ar:11336/166058instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:19:48.236CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
title Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
spellingShingle Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
Andreozzi, Patrizia
BIOLOGICAL FATE
POLYAMINE PHOSPHATE NANOPARTICLES
POLYETHYLENE GLYCOL
SELF ASSEMBLY
SMALL ANGLE X-RAY SCATTERING
title_short Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
title_full Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
title_fullStr Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
title_full_unstemmed Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
title_sort Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time
dc.creator.none.fl_str_mv Andreozzi, Patrizia
Simó, Cristina
Moretti, Paolo
Martinez Porcel, Joaquin
Lüdtke, Tanja Ursula
Ramirez, Maria de Los Angeles
Tamberi, Lorenza
Marradi, Marco
Amenitsch, Heinz
Llop, Jordi
Ortore, Maria Grazia
Moya, Sergio Enrique
author Andreozzi, Patrizia
author_facet Andreozzi, Patrizia
Simó, Cristina
Moretti, Paolo
Martinez Porcel, Joaquin
Lüdtke, Tanja Ursula
Ramirez, Maria de Los Angeles
Tamberi, Lorenza
Marradi, Marco
Amenitsch, Heinz
Llop, Jordi
Ortore, Maria Grazia
Moya, Sergio Enrique
author_role author
author2 Simó, Cristina
Moretti, Paolo
Martinez Porcel, Joaquin
Lüdtke, Tanja Ursula
Ramirez, Maria de Los Angeles
Tamberi, Lorenza
Marradi, Marco
Amenitsch, Heinz
Llop, Jordi
Ortore, Maria Grazia
Moya, Sergio Enrique
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL FATE
POLYAMINE PHOSPHATE NANOPARTICLES
POLYETHYLENE GLYCOL
SELF ASSEMBLY
SMALL ANGLE X-RAY SCATTERING
topic BIOLOGICAL FATE
POLYAMINE PHOSPHATE NANOPARTICLES
POLYETHYLENE GLYCOL
SELF ASSEMBLY
SMALL ANGLE X-RAY SCATTERING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An approach for reducing toxicity and enhancing therapeutic potential of supramolecular polyamine phosphate nanoparticles (PANs) through PEGylation of polyamines before their assembly into nanoparticles is presented here. It is shown that the number of polyethylene glycol (PEG) chains for polyamine largely influence physico-chemical properties of PANs and their biological endpoints. Poly(allylamine hydrochloride) (PAH) are functionalized through carbodiimide chemistry with three ratios of PEG molecules per PAH chain: 0.1, 1, and 10. PEGylated PAH is then assembled into PANs by exposing the polymer to phosphate buffer solution. PANs decrease size and surface charge with increasing PEG ratios as evidenced by dynamic light scattering and zeta potential measurements, with the ten PEG/PAH ratio PANs having practically zero charge. Small angle X-ray scattering (SAXS) proves that PEG chains form a shell around a polyamine core, which is responsible for the screening of positive charges. MTT experiments show that the screening of amine groups decreases nanoparticle toxicity, with the lowest toxicity for the 10 PEG/PAH ratio. Fluorescence correlation spectroscopy (FCS) proves less interaction with proteins for PEGylated PANs. Positron emission tomography (PET) imaging of 18F labelled PANs shows longer circulation time in healthy mice for PEGylated PANs than non-PEGylated ones.
Fil: Andreozzi, Patrizia. Basque Research and Technology Alliance; España. Università degli Studi di Firenze; Italia
Fil: Simó, Cristina. Basque Research and Technology Alliance; España
Fil: Moretti, Paolo. Università Politecnica delle Marche; Italia
Fil: Martinez Porcel, Joaquin. Basque Research and Technology Alliance; España
Fil: Lüdtke, Tanja Ursula. Basque Research and Technology Alliance; España
Fil: Ramirez, Maria de Los Angeles. Basque Research and Technology Alliance; España. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tamberi, Lorenza. Basque Research and Technology Alliance; España
Fil: Marradi, Marco. Università degli Studi di Firenze; Italia
Fil: Amenitsch, Heinz. Graz University Of Technology.; Austria
Fil: Llop, Jordi. Basque Research and Technology Alliance; España. Centro de Investigación Biomédica En Red de Enfermedades Respiratorias; España
Fil: Ortore, Maria Grazia. Università Politecnica Delle Marche; Italia
Fil: Moya, Sergio Enrique. Basque Research and Technology Alliance; España
description An approach for reducing toxicity and enhancing therapeutic potential of supramolecular polyamine phosphate nanoparticles (PANs) through PEGylation of polyamines before their assembly into nanoparticles is presented here. It is shown that the number of polyethylene glycol (PEG) chains for polyamine largely influence physico-chemical properties of PANs and their biological endpoints. Poly(allylamine hydrochloride) (PAH) are functionalized through carbodiimide chemistry with three ratios of PEG molecules per PAH chain: 0.1, 1, and 10. PEGylated PAH is then assembled into PANs by exposing the polymer to phosphate buffer solution. PANs decrease size and surface charge with increasing PEG ratios as evidenced by dynamic light scattering and zeta potential measurements, with the ten PEG/PAH ratio PANs having practically zero charge. Small angle X-ray scattering (SAXS) proves that PEG chains form a shell around a polyamine core, which is responsible for the screening of positive charges. MTT experiments show that the screening of amine groups decreases nanoparticle toxicity, with the lowest toxicity for the 10 PEG/PAH ratio. Fluorescence correlation spectroscopy (FCS) proves less interaction with proteins for PEGylated PANs. Positron emission tomography (PET) imaging of 18F labelled PANs shows longer circulation time in healthy mice for PEGylated PANs than non-PEGylated ones.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/166058
Andreozzi, Patrizia; Simó, Cristina; Moretti, Paolo; Martinez Porcel, Joaquin; Lüdtke, Tanja Ursula; et al.; Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time; Wiley VCH Verlag; Small; 17; 35; 2-9-2021; 1-10
1613-6810
1613-6829
CONICET Digital
CONICET
url http://hdl.handle.net/11336/166058
identifier_str_mv Andreozzi, Patrizia; Simó, Cristina; Moretti, Paolo; Martinez Porcel, Joaquin; Lüdtke, Tanja Ursula; et al.; Novel Core–Shell Polyamine Phosphate Nanoparticles Self-Assembled from PEGylated Poly(allylamine hydrochloride) with Low Toxicity and Increased In Vivo Circulation Time; Wiley VCH Verlag; Small; 17; 35; 2-9-2021; 1-10
1613-6810
1613-6829
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/smll.202102211
info:eu-repo/semantics/altIdentifier/doi/10.1002/smll.202102211
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.121305

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