Triggering doxorubicin release from responsive hydrogel films by polyamine uptake

Autores
Pérez Chávez, Néstor Ariel; Nosthas Aguiar, Victor; Allegretto, Juan Alejandro; Albesa, Alberto Gustavo; Giussi, Juan Martín; Longo, Gabriel Sebastian
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyamines such as putrescine, spermidine and spermine are required in many inter- and intra-cellular processes. There is, however, evidence of anomalously high concentrations of these polyamines around cancer cells. Furthermore, high polyamine concentrations play a key role in accelerating the speed of cancer proliferation. Some current therapies target the reduction of the polyamine concentration to delay the cancer advance. In this study, we use a molecular theory to prove the concept that poly(methacrylic acid) (PMAA) hydrogels can play the dual role of incorporating and retaining polyamines as well as releasing preloaded drugs in response. Towards such a goal, we have developed a molecular model for each of the chemical species, which includes the shape, size, charge, protonation state, and configuration. Our results indicate that PMAA hydrogel films can incorporate significant amounts of polyamines; this absorption increases with the solution concentration of the polyamines. Doxorubicin was chosen as a model drug for this study, which can be successfully incorporated within the film; the optimal encapsulation conditions occur at low salt concentrations and pH values near neutral. Polyamine absorption within the film results in the desorption of the drug from the hydrogel. An increase in the concentration of the polyamines enhances the drug release. To validate our theoretical findings, poly(methacrylic acid) hydrogel thin films were synthesized by atom transfer radical polymerization. Absorption/desorption experiments followed by UV-Vis spectroscopy demonstrate doxorubicin encapsulation within these films and polyamine-dependent drug release. This journal is
Fil: Pérez Chávez, Néstor Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Nosthas Aguiar, Victor. Universidad Nacional de La Plata; Argentina
Fil: Allegretto, Juan Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Albesa, Alberto Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Giussi, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Longo, Gabriel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
Calculation
Hydrogel
Doxorubicin
Polyamines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141719
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141719
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Triggering doxorubicin release from responsive hydrogel films by polyamine uptakePérez Chávez, Néstor ArielNosthas Aguiar, VictorAllegretto, Juan AlejandroAlbesa, Alberto GustavoGiussi, Juan MartínLongo, Gabriel SebastianCalculationHydrogelDoxorubicinPolyamineshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Polyamines such as putrescine, spermidine and spermine are required in many inter- and intra-cellular processes. There is, however, evidence of anomalously high concentrations of these polyamines around cancer cells. Furthermore, high polyamine concentrations play a key role in accelerating the speed of cancer proliferation. Some current therapies target the reduction of the polyamine concentration to delay the cancer advance. In this study, we use a molecular theory to prove the concept that poly(methacrylic acid) (PMAA) hydrogels can play the dual role of incorporating and retaining polyamines as well as releasing preloaded drugs in response. Towards such a goal, we have developed a molecular model for each of the chemical species, which includes the shape, size, charge, protonation state, and configuration. Our results indicate that PMAA hydrogel films can incorporate significant amounts of polyamines; this absorption increases with the solution concentration of the polyamines. Doxorubicin was chosen as a model drug for this study, which can be successfully incorporated within the film; the optimal encapsulation conditions occur at low salt concentrations and pH values near neutral. Polyamine absorption within the film results in the desorption of the drug from the hydrogel. An increase in the concentration of the polyamines enhances the drug release. To validate our theoretical findings, poly(methacrylic acid) hydrogel thin films were synthesized by atom transfer radical polymerization. Absorption/desorption experiments followed by UV-Vis spectroscopy demonstrate doxorubicin encapsulation within these films and polyamine-dependent drug release. This journal isFil: Pérez Chávez, Néstor Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Nosthas Aguiar, Victor. Universidad Nacional de La Plata; ArgentinaFil: Allegretto, Juan Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Albesa, Alberto Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Giussi, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Longo, Gabriel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaRoyal Society of Chemistry2020-07-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141719Pérez Chávez, Néstor Ariel; Nosthas Aguiar, Victor; Allegretto, Juan Alejandro; Albesa, Alberto Gustavo; Giussi, Juan Martín; et al.; Triggering doxorubicin release from responsive hydrogel films by polyamine uptake; Royal Society of Chemistry; Soft Matter; 16; 32; 14-7-2020; 7492-75021744-683X1744-6848CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2020/SM/D0SM00951Binfo:eu-repo/semantics/altIdentifier/doi/10.1039/D0SM00951Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:04Zoai:ri.conicet.gov.ar:11336/141719instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:04.514CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
title Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
spellingShingle Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
Pérez Chávez, Néstor Ariel
Calculation
Hydrogel
Doxorubicin
Polyamines
title_short Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
title_full Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
title_fullStr Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
title_full_unstemmed Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
title_sort Triggering doxorubicin release from responsive hydrogel films by polyamine uptake
dc.creator.none.fl_str_mv Pérez Chávez, Néstor Ariel
Nosthas Aguiar, Victor
Allegretto, Juan Alejandro
Albesa, Alberto Gustavo
Giussi, Juan Martín
Longo, Gabriel Sebastian
author Pérez Chávez, Néstor Ariel
author_facet Pérez Chávez, Néstor Ariel
Nosthas Aguiar, Victor
Allegretto, Juan Alejandro
Albesa, Alberto Gustavo
Giussi, Juan Martín
Longo, Gabriel Sebastian
author_role author
author2 Nosthas Aguiar, Victor
Allegretto, Juan Alejandro
Albesa, Alberto Gustavo
Giussi, Juan Martín
Longo, Gabriel Sebastian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Calculation
Hydrogel
Doxorubicin
Polyamines
topic Calculation
Hydrogel
Doxorubicin
Polyamines
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polyamines such as putrescine, spermidine and spermine are required in many inter- and intra-cellular processes. There is, however, evidence of anomalously high concentrations of these polyamines around cancer cells. Furthermore, high polyamine concentrations play a key role in accelerating the speed of cancer proliferation. Some current therapies target the reduction of the polyamine concentration to delay the cancer advance. In this study, we use a molecular theory to prove the concept that poly(methacrylic acid) (PMAA) hydrogels can play the dual role of incorporating and retaining polyamines as well as releasing preloaded drugs in response. Towards such a goal, we have developed a molecular model for each of the chemical species, which includes the shape, size, charge, protonation state, and configuration. Our results indicate that PMAA hydrogel films can incorporate significant amounts of polyamines; this absorption increases with the solution concentration of the polyamines. Doxorubicin was chosen as a model drug for this study, which can be successfully incorporated within the film; the optimal encapsulation conditions occur at low salt concentrations and pH values near neutral. Polyamine absorption within the film results in the desorption of the drug from the hydrogel. An increase in the concentration of the polyamines enhances the drug release. To validate our theoretical findings, poly(methacrylic acid) hydrogel thin films were synthesized by atom transfer radical polymerization. Absorption/desorption experiments followed by UV-Vis spectroscopy demonstrate doxorubicin encapsulation within these films and polyamine-dependent drug release. This journal is
Fil: Pérez Chávez, Néstor Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Nosthas Aguiar, Victor. Universidad Nacional de La Plata; Argentina
Fil: Allegretto, Juan Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Albesa, Alberto Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Giussi, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Longo, Gabriel Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description Polyamines such as putrescine, spermidine and spermine are required in many inter- and intra-cellular processes. There is, however, evidence of anomalously high concentrations of these polyamines around cancer cells. Furthermore, high polyamine concentrations play a key role in accelerating the speed of cancer proliferation. Some current therapies target the reduction of the polyamine concentration to delay the cancer advance. In this study, we use a molecular theory to prove the concept that poly(methacrylic acid) (PMAA) hydrogels can play the dual role of incorporating and retaining polyamines as well as releasing preloaded drugs in response. Towards such a goal, we have developed a molecular model for each of the chemical species, which includes the shape, size, charge, protonation state, and configuration. Our results indicate that PMAA hydrogel films can incorporate significant amounts of polyamines; this absorption increases with the solution concentration of the polyamines. Doxorubicin was chosen as a model drug for this study, which can be successfully incorporated within the film; the optimal encapsulation conditions occur at low salt concentrations and pH values near neutral. Polyamine absorption within the film results in the desorption of the drug from the hydrogel. An increase in the concentration of the polyamines enhances the drug release. To validate our theoretical findings, poly(methacrylic acid) hydrogel thin films were synthesized by atom transfer radical polymerization. Absorption/desorption experiments followed by UV-Vis spectroscopy demonstrate doxorubicin encapsulation within these films and polyamine-dependent drug release. This journal is
publishDate 2020
dc.date.none.fl_str_mv 2020-07-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141719
Pérez Chávez, Néstor Ariel; Nosthas Aguiar, Victor; Allegretto, Juan Alejandro; Albesa, Alberto Gustavo; Giussi, Juan Martín; et al.; Triggering doxorubicin release from responsive hydrogel films by polyamine uptake; Royal Society of Chemistry; Soft Matter; 16; 32; 14-7-2020; 7492-7502
1744-683X
1744-6848
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141719
identifier_str_mv Pérez Chávez, Néstor Ariel; Nosthas Aguiar, Victor; Allegretto, Juan Alejandro; Albesa, Alberto Gustavo; Giussi, Juan Martín; et al.; Triggering doxorubicin release from responsive hydrogel films by polyamine uptake; Royal Society of Chemistry; Soft Matter; 16; 32; 14-7-2020; 7492-7502
1744-683X
1744-6848
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/content/articlelanding/2020/SM/D0SM00951B
info:eu-repo/semantics/altIdentifier/doi/10.1039/D0SM00951B
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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