Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation

Autores
Hidalgo, María Eugenia; Folmer Correa, Ana Paula; Mancilla Canales, Manuel Arturo; Joner Daroit, Daniel; Brandelli, Adriano; Risso, Patricia Hilda
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates were evaluated. An acid-induced aggregation process of the hydrolysates and their corresponding mixtures with NaCAS were also analyzed. The results showed that the enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to aggregate by addition of glucono-delta-lactone, and their incorporation into NaCAS solutions alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, estimated by the fractal dimension of aggregates, was not significantly altered by the incorporation of hydrolysates. However, at higher protein concentrations, when the decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates alters the microstructure and rheological behavior of these gels.
Fil: Hidalgo, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
Fil: Folmer Correa, Ana Paula. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancilla Canales, Manuel Arturo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
Fil: Joner Daroit, Daniel. Universidade Federal da Fronteira Sul; Brasil
Fil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Risso, Patricia Hilda. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
Materia
Bacillus Sp.P7
Bovine Sodium Caseinate
Hydrolysates
Bioactivity
Acid Agreggation And Gelation
Microstructure
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6194
Acceder
id CONICETDig_c90fd53de08c60293baa08e49809f807
oai_identifier_str oai:ri.conicet.gov.ar:11336/6194
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparationHidalgo, María EugeniaFolmer Correa, Ana PaulaMancilla Canales, Manuel ArturoJoner Daroit, DanielBrandelli, AdrianoRisso, Patricia HildaBacillus Sp.P7Bovine Sodium CaseinateHydrolysatesBioactivityAcid Agreggation And GelationMicrostructurehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates were evaluated. An acid-induced aggregation process of the hydrolysates and their corresponding mixtures with NaCAS were also analyzed. The results showed that the enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to aggregate by addition of glucono-delta-lactone, and their incorporation into NaCAS solutions alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, estimated by the fractal dimension of aggregates, was not significantly altered by the incorporation of hydrolysates. However, at higher protein concentrations, when the decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates alters the microstructure and rheological behavior of these gels.Fil: Hidalgo, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaFil: Folmer Correa, Ana Paula. Universidade Federal do Rio Grande do Sul; BrasilFil: Mancilla Canales, Manuel Arturo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaFil: Joner Daroit, Daniel. Universidade Federal da Fronteira Sul; BrasilFil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; BrasilFil: Risso, Patricia Hilda. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); ArgentinaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6194Hidalgo, María Eugenia; Folmer Correa, Ana Paula; Mancilla Canales, Manuel Arturo; Joner Daroit, Daniel; Brandelli, Adriano; et al.; Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation; Elsevier; Food Hydrocolloids; 43; 7-2014; 510-5200268-005Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X14002525info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2014.07.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:29:23Zoai:ri.conicet.gov.ar:11336/6194instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:29:24.116CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
spellingShingle Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
Hidalgo, María Eugenia
Bacillus Sp.P7
Bovine Sodium Caseinate
Hydrolysates
Bioactivity
Acid Agreggation And Gelation
Microstructure
title_short Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_full Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_fullStr Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_full_unstemmed Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
title_sort Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation
dc.creator.none.fl_str_mv Hidalgo, María Eugenia
Folmer Correa, Ana Paula
Mancilla Canales, Manuel Arturo
Joner Daroit, Daniel
Brandelli, Adriano
Risso, Patricia Hilda
author Hidalgo, María Eugenia
author_facet Hidalgo, María Eugenia
Folmer Correa, Ana Paula
Mancilla Canales, Manuel Arturo
Joner Daroit, Daniel
Brandelli, Adriano
Risso, Patricia Hilda
author_role author
author2 Folmer Correa, Ana Paula
Mancilla Canales, Manuel Arturo
Joner Daroit, Daniel
Brandelli, Adriano
Risso, Patricia Hilda
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacillus Sp.P7
Bovine Sodium Caseinate
Hydrolysates
Bioactivity
Acid Agreggation And Gelation
Microstructure
topic Bacillus Sp.P7
Bovine Sodium Caseinate
Hydrolysates
Bioactivity
Acid Agreggation And Gelation
Microstructure
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates were evaluated. An acid-induced aggregation process of the hydrolysates and their corresponding mixtures with NaCAS were also analyzed. The results showed that the enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to aggregate by addition of glucono-delta-lactone, and their incorporation into NaCAS solutions alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, estimated by the fractal dimension of aggregates, was not significantly altered by the incorporation of hydrolysates. However, at higher protein concentrations, when the decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates alters the microstructure and rheological behavior of these gels.
Fil: Hidalgo, María Eugenia. Universidad Nacional de Rosario. Facultad de Cs.bioquimicas y Farmaceuticas. Departamento de Quimica y Fisica. Area Fisicoquimica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
Fil: Folmer Correa, Ana Paula. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancilla Canales, Manuel Arturo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
Fil: Joner Daroit, Daniel. Universidade Federal da Fronteira Sul; Brasil
Fil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Risso, Patricia Hilda. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Física de Rosario (i); Argentina
description In this work, we aimed at the production of bovine sodium caseinate (NaCAS) hydrolysates by means of an extracellular protease from Bacillus sp. P7. Mass spectrometry was carried out to evaluate peptide mass distribution and identified sequences of peptides with a signal/noise ratio higher than 10. Antioxidant and antimicrobial properties of hydrolysates were evaluated. An acid-induced aggregation process of the hydrolysates and their corresponding mixtures with NaCAS were also analyzed. The results showed that the enzymatic hydrolysis produced peptides, mostly lower than 3 kDa, with different bioactivities depending on the time of hydrolysis (ti). These hydrolysates lost their ability to aggregate by addition of glucono-delta-lactone, and their incorporation into NaCAS solutions alter the kinetics of the process. Also, the degree of compactness of the NaCAS aggregates, estimated by the fractal dimension of aggregates, was not significantly altered by the incorporation of hydrolysates. However, at higher protein concentrations, when the decrease in pH leads to the formation of NaCAS acid gels, the presence of hydrolysates alters the microstructure and rheological behavior of these gels.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6194
Hidalgo, María Eugenia; Folmer Correa, Ana Paula; Mancilla Canales, Manuel Arturo; Joner Daroit, Daniel; Brandelli, Adriano; et al.; Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation; Elsevier; Food Hydrocolloids; 43; 7-2014; 510-520
0268-005X
url http://hdl.handle.net/11336/6194
identifier_str_mv Hidalgo, María Eugenia; Folmer Correa, Ana Paula; Mancilla Canales, Manuel Arturo; Joner Daroit, Daniel; Brandelli, Adriano; et al.; Biological and physicochemical properties of bovine sodium caseinate hydrolysates obtained by a bacterial protease preparation; Elsevier; Food Hydrocolloids; 43; 7-2014; 510-520
0268-005X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0268005X14002525
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodhyd.2014.07.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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