Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells

Autores
Novaira, Mercedes; Cormick, Maria Paula; Durantini, Edgardo Néstor
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Spectroscopic and time-resolved fluorescence emission techniques were used to provide information for the interaction of 5,10,15,20-tetrakis(4-N,N,N- trimethylammoniumphenyl) porphyrin (TMAP 4+) and 5,10,15,20- tetrakis(4-sulphonatophenyl) porphyrin (TPPS 4-) with different biomimetic media and with Candida albicans cells. In n-heptane/sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/water and benzene/benzyl-n- hexadecyldimethylammonium chloride (BHDC)/water reverse micelles interactions were dependent on the micellar interface and the amount of water dispersed in the microemulsion. It was also observed that the DNA binding of cationic porphyrin TMAP 4+ led to two lifetimes. In vitro investigations showed that TMAP 4+ is bound to C. albicans. Fluorescence lifetime measurements and fluorescence microscopic images provided additional insight into the effects of porphyrin uptake by cells. The results reveal a double localization of TMAP 4+ inside of C. albicans cells. Thus, a redistribution of TMAP 4+ was observed in unwashed cells, probably due to a relocalisation of molecules that were weakly bound to the cells or remained in solution. However, this effect was not found with molecules tightly bound in the cells, after one washing step.
Fil: Novaira, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina
Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina
Materia
CANDIDA ALBICANS
DNA
FLUORESCENCE
MICELLES
PORPHYRIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/189177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cellsNovaira, MercedesCormick, Maria PaulaDurantini, Edgardo NéstorCANDIDA ALBICANSDNAFLUORESCENCEMICELLESPORPHYRINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Spectroscopic and time-resolved fluorescence emission techniques were used to provide information for the interaction of 5,10,15,20-tetrakis(4-N,N,N- trimethylammoniumphenyl) porphyrin (TMAP 4+) and 5,10,15,20- tetrakis(4-sulphonatophenyl) porphyrin (TPPS 4-) with different biomimetic media and with Candida albicans cells. In n-heptane/sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/water and benzene/benzyl-n- hexadecyldimethylammonium chloride (BHDC)/water reverse micelles interactions were dependent on the micellar interface and the amount of water dispersed in the microemulsion. It was also observed that the DNA binding of cationic porphyrin TMAP 4+ led to two lifetimes. In vitro investigations showed that TMAP 4+ is bound to C. albicans. Fluorescence lifetime measurements and fluorescence microscopic images provided additional insight into the effects of porphyrin uptake by cells. The results reveal a double localization of TMAP 4+ inside of C. albicans cells. Thus, a redistribution of TMAP 4+ was observed in unwashed cells, probably due to a relocalisation of molecules that were weakly bound to the cells or remained in solution. However, this effect was not found with molecules tightly bound in the cells, after one washing step.Fil: Novaira, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; ArgentinaFil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; ArgentinaElsevier Science SA2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189177Novaira, Mercedes; Cormick, Maria Paula; Durantini, Edgardo Néstor; Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 246; 8-2012; 67-741010-6030CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1010603012003632info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2012.06.024info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:46:41Zoai:ri.conicet.gov.ar:11336/189177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:46:41.866CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
title Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
spellingShingle Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
Novaira, Mercedes
CANDIDA ALBICANS
DNA
FLUORESCENCE
MICELLES
PORPHYRIN
title_short Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
title_full Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
title_fullStr Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
title_full_unstemmed Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
title_sort Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells
dc.creator.none.fl_str_mv Novaira, Mercedes
Cormick, Maria Paula
Durantini, Edgardo Néstor
author Novaira, Mercedes
author_facet Novaira, Mercedes
Cormick, Maria Paula
Durantini, Edgardo Néstor
author_role author
author2 Cormick, Maria Paula
Durantini, Edgardo Néstor
author2_role author
author
dc.subject.none.fl_str_mv CANDIDA ALBICANS
DNA
FLUORESCENCE
MICELLES
PORPHYRIN
topic CANDIDA ALBICANS
DNA
FLUORESCENCE
MICELLES
PORPHYRIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Spectroscopic and time-resolved fluorescence emission techniques were used to provide information for the interaction of 5,10,15,20-tetrakis(4-N,N,N- trimethylammoniumphenyl) porphyrin (TMAP 4+) and 5,10,15,20- tetrakis(4-sulphonatophenyl) porphyrin (TPPS 4-) with different biomimetic media and with Candida albicans cells. In n-heptane/sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/water and benzene/benzyl-n- hexadecyldimethylammonium chloride (BHDC)/water reverse micelles interactions were dependent on the micellar interface and the amount of water dispersed in the microemulsion. It was also observed that the DNA binding of cationic porphyrin TMAP 4+ led to two lifetimes. In vitro investigations showed that TMAP 4+ is bound to C. albicans. Fluorescence lifetime measurements and fluorescence microscopic images provided additional insight into the effects of porphyrin uptake by cells. The results reveal a double localization of TMAP 4+ inside of C. albicans cells. Thus, a redistribution of TMAP 4+ was observed in unwashed cells, probably due to a relocalisation of molecules that were weakly bound to the cells or remained in solution. However, this effect was not found with molecules tightly bound in the cells, after one washing step.
Fil: Novaira, Mercedes. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina
Fil: Durantini, Edgardo Néstor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química y Física; Argentina
description Spectroscopic and time-resolved fluorescence emission techniques were used to provide information for the interaction of 5,10,15,20-tetrakis(4-N,N,N- trimethylammoniumphenyl) porphyrin (TMAP 4+) and 5,10,15,20- tetrakis(4-sulphonatophenyl) porphyrin (TPPS 4-) with different biomimetic media and with Candida albicans cells. In n-heptane/sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/water and benzene/benzyl-n- hexadecyldimethylammonium chloride (BHDC)/water reverse micelles interactions were dependent on the micellar interface and the amount of water dispersed in the microemulsion. It was also observed that the DNA binding of cationic porphyrin TMAP 4+ led to two lifetimes. In vitro investigations showed that TMAP 4+ is bound to C. albicans. Fluorescence lifetime measurements and fluorescence microscopic images provided additional insight into the effects of porphyrin uptake by cells. The results reveal a double localization of TMAP 4+ inside of C. albicans cells. Thus, a redistribution of TMAP 4+ was observed in unwashed cells, probably due to a relocalisation of molecules that were weakly bound to the cells or remained in solution. However, this effect was not found with molecules tightly bound in the cells, after one washing step.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189177
Novaira, Mercedes; Cormick, Maria Paula; Durantini, Edgardo Néstor; Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 246; 8-2012; 67-74
1010-6030
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189177
identifier_str_mv Novaira, Mercedes; Cormick, Maria Paula; Durantini, Edgardo Néstor; Spectroscopic and time-resolved fluorescence emission properties of a cationic and an anionic porphyrin in biomimetic media and Candida albicans cells; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 246; 8-2012; 67-74
1010-6030
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1010603012003632
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2012.06.024
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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