Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives

Autores
Quiroga, Ezequiel Dario; Cormick, Maria Paula; Pons, Patricia; Alvarez, María Gabriela; Durantini, Edgardo Néstor
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Photodynamic inactivation of Candida albicans produced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl) porphyrin (TMAP4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin (TMPyP4+) was investigated to obtain insight about the mechanism of cellular damage. In solution, absorption spectroscopic studies showed that these cationic porphyrins interact strongly with calf thymus DNA. The electrophoretic analysis indicated that photocleavage of DNA induced by TFAP3+ took place after long irradiation periods (>5 h). In contrast, TMAP4+ produced a marked reduction in DNA band after 1 h irradiation. In C. albicans, these cationic porphyrins produced a ∼3.5 log decrease in survival when the cell suspensions (107 cells/mL) were incubated with 5 μM photosensitizer and irradiated for 30 min with visible light (fluence 162 J/cm2). After this treatment, modifications of genomic DNA isolated from C. albicans cells were not found by electrophoresis. Furthermore, transmission electron microscopy showed structural changes with appearance of low density areas into the cells and irregularities in cell barriers. However, the photodamage to the cell envelope was insufficient to cause the release of intracellular biopolymers. Therefore, modifications in the cytoplasmic biomolecules and alteration in the cell barriers could be mainly involved in C. albicans photoinactivation.
Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Pons, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electrónica; Argentina
Fil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
Antifungal
Candida Albicans
Cationic Porphyrin
Dna
Photoinactivation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84125
Acceder
id CONICETDig_daf298841839e595429aeedd9d876d3f
oai_identifier_str oai:ri.conicet.gov.ar:11336/84125
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivativesQuiroga, Ezequiel DarioCormick, Maria PaulaPons, PatriciaAlvarez, María GabrielaDurantini, Edgardo NéstorAntifungalCandida AlbicansCationic PorphyrinDnaPhotoinactivationPhotodynamic inactivation of Candida albicans produced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl) porphyrin (TMAP4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin (TMPyP4+) was investigated to obtain insight about the mechanism of cellular damage. In solution, absorption spectroscopic studies showed that these cationic porphyrins interact strongly with calf thymus DNA. The electrophoretic analysis indicated that photocleavage of DNA induced by TFAP3+ took place after long irradiation periods (>5 h). In contrast, TMAP4+ produced a marked reduction in DNA band after 1 h irradiation. In C. albicans, these cationic porphyrins produced a ∼3.5 log decrease in survival when the cell suspensions (107 cells/mL) were incubated with 5 μM photosensitizer and irradiated for 30 min with visible light (fluence 162 J/cm2). After this treatment, modifications of genomic DNA isolated from C. albicans cells were not found by electrophoresis. Furthermore, transmission electron microscopy showed structural changes with appearance of low density areas into the cells and irregularities in cell barriers. However, the photodamage to the cell envelope was insufficient to cause the release of intracellular biopolymers. Therefore, modifications in the cytoplasmic biomolecules and alteration in the cell barriers could be mainly involved in C. albicans photoinactivation.Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Pons, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electrónica; ArgentinaFil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84125Quiroga, Ezequiel Dario; Cormick, Maria Paula; Pons, Patricia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives; Elsevier France-editions Scientifiques Medicales Elsevier; European Journal of Medical Chemistry; 58; 12-2012; 332-3390223-5234CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0223523412006216info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejmech.2012.10.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:44Zoai:ri.conicet.gov.ar:11336/84125instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:44.361CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
title Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
spellingShingle Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
Quiroga, Ezequiel Dario
Antifungal
Candida Albicans
Cationic Porphyrin
Dna
Photoinactivation
title_short Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
title_full Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
title_fullStr Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
title_full_unstemmed Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
title_sort Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives
dc.creator.none.fl_str_mv Quiroga, Ezequiel Dario
Cormick, Maria Paula
Pons, Patricia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author Quiroga, Ezequiel Dario
author_facet Quiroga, Ezequiel Dario
Cormick, Maria Paula
Pons, Patricia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author_role author
author2 Cormick, Maria Paula
Pons, Patricia
Alvarez, María Gabriela
Durantini, Edgardo Néstor
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antifungal
Candida Albicans
Cationic Porphyrin
Dna
Photoinactivation
topic Antifungal
Candida Albicans
Cationic Porphyrin
Dna
Photoinactivation
dc.description.none.fl_txt_mv Photodynamic inactivation of Candida albicans produced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl) porphyrin (TMAP4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin (TMPyP4+) was investigated to obtain insight about the mechanism of cellular damage. In solution, absorption spectroscopic studies showed that these cationic porphyrins interact strongly with calf thymus DNA. The electrophoretic analysis indicated that photocleavage of DNA induced by TFAP3+ took place after long irradiation periods (>5 h). In contrast, TMAP4+ produced a marked reduction in DNA band after 1 h irradiation. In C. albicans, these cationic porphyrins produced a ∼3.5 log decrease in survival when the cell suspensions (107 cells/mL) were incubated with 5 μM photosensitizer and irradiated for 30 min with visible light (fluence 162 J/cm2). After this treatment, modifications of genomic DNA isolated from C. albicans cells were not found by electrophoresis. Furthermore, transmission electron microscopy showed structural changes with appearance of low density areas into the cells and irregularities in cell barriers. However, the photodamage to the cell envelope was insufficient to cause the release of intracellular biopolymers. Therefore, modifications in the cytoplasmic biomolecules and alteration in the cell barriers could be mainly involved in C. albicans photoinactivation.
Fil: Quiroga, Ezequiel Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Cormick, Maria Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Pons, Patricia. Universidad Nacional de Córdoba. Facultad de Medicina. Centro de Microscopia Electrónica; Argentina
Fil: Alvarez, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Durantini, Edgardo Néstor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Photodynamic inactivation of Candida albicans produced by 5-(4-trifluorophenyl)-10,15,20-tris(4-N,N,N-trimethylammoniumphenyl)porphyrin (TFAP3+), 5,10,15,20-tetrakis(4-N,N,N-trimethylammoniumphenyl) porphyrin (TMAP4+) and 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin (TMPyP4+) was investigated to obtain insight about the mechanism of cellular damage. In solution, absorption spectroscopic studies showed that these cationic porphyrins interact strongly with calf thymus DNA. The electrophoretic analysis indicated that photocleavage of DNA induced by TFAP3+ took place after long irradiation periods (>5 h). In contrast, TMAP4+ produced a marked reduction in DNA band after 1 h irradiation. In C. albicans, these cationic porphyrins produced a ∼3.5 log decrease in survival when the cell suspensions (107 cells/mL) were incubated with 5 μM photosensitizer and irradiated for 30 min with visible light (fluence 162 J/cm2). After this treatment, modifications of genomic DNA isolated from C. albicans cells were not found by electrophoresis. Furthermore, transmission electron microscopy showed structural changes with appearance of low density areas into the cells and irregularities in cell barriers. However, the photodamage to the cell envelope was insufficient to cause the release of intracellular biopolymers. Therefore, modifications in the cytoplasmic biomolecules and alteration in the cell barriers could be mainly involved in C. albicans photoinactivation.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84125
Quiroga, Ezequiel Dario; Cormick, Maria Paula; Pons, Patricia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives; Elsevier France-editions Scientifiques Medicales Elsevier; European Journal of Medical Chemistry; 58; 12-2012; 332-339
0223-5234
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84125
identifier_str_mv Quiroga, Ezequiel Dario; Cormick, Maria Paula; Pons, Patricia; Alvarez, María Gabriela; Durantini, Edgardo Néstor; Mechanistic aspects of the photodynamic inactivation of Candida albicans induced by cationic porphyrin derivatives; Elsevier France-editions Scientifiques Medicales Elsevier; European Journal of Medical Chemistry; 58; 12-2012; 332-339
0223-5234
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0223523412006216
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejmech.2012.10.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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