Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina

Autores
Rosa, Silvina Mariana; Galvagno, Miguel Angel; Vélez, Carlos Guillermo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable elusive thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments.
Fil: Rosa, Silvina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Vélez, Carlos Guillermo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
Alternative isolation procedures
Elusive taxa
Media formulation
Thraustochytriales
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280900
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280900
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern ArgentinaRosa, Silvina MarianaGalvagno, Miguel AngelVélez, Carlos GuillermoAlternative isolation proceduresElusive taxaMedia formulationThraustochytrialeshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable elusive thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments.Fil: Rosa, Silvina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Vélez, Carlos Guillermo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaSpringer Tokyo2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280900Rosa, Silvina Mariana; Galvagno, Miguel Angel; Vélez, Carlos Guillermo; Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina; Springer Tokyo; Mycoscience; 52; 4; 7-2011; 242-2521340-3540CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10267-010-0091-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-06T13:35:00Zoai:ri.conicet.gov.ar:11336/280900instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-06 13:35:00.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
title Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
spellingShingle Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
Rosa, Silvina Mariana
Alternative isolation procedures
Elusive taxa
Media formulation
Thraustochytriales
title_short Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
title_full Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
title_fullStr Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
title_full_unstemmed Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
title_sort Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina
dc.creator.none.fl_str_mv Rosa, Silvina Mariana
Galvagno, Miguel Angel
Vélez, Carlos Guillermo
author Rosa, Silvina Mariana
author_facet Rosa, Silvina Mariana
Galvagno, Miguel Angel
Vélez, Carlos Guillermo
author_role author
author2 Galvagno, Miguel Angel
Vélez, Carlos Guillermo
author2_role author
author
dc.subject.none.fl_str_mv Alternative isolation procedures
Elusive taxa
Media formulation
Thraustochytriales
topic Alternative isolation procedures
Elusive taxa
Media formulation
Thraustochytriales
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable elusive thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments.
Fil: Rosa, Silvina Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
Fil: Vélez, Carlos Guillermo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description To study thraustochytrids from temperate and cold environments of Southern Argentina, the standard cultivation methodologies have been modified because many of the microorganisms detected by microscopic examination in both the original samples and the colonized baits failed to be successfully isolated in standard culture media. As a result, 35 strains, most of them having a very low growth rate, were isolated. Alternative procedures are proposed according to the nature of the sample, the characteristics of the thraustochytrid to be isolated, and the presence of contaminating microorganisms. Modifications proposed include the use of a newly formulated culture medium (Mar Chiquita, containing glucose, gelatine hydrolysate, peptone, and corn steep liquor as main carbon and nitrogen sources). In addition, the effects of the nutrient composition and agar concentration of culture media on the relative growth rates of the isolates were studied in an attempt to determine the most suitable conditions for the cultivation of new strains of thraustochytrids. The goal of this study is to develop a standard methodology, allowing us to grow baitable elusive thraustochytrid strains, and that could be applied to improve the isolation and the study of the undocumented biodiversity of this group of microorganisms from different environments.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280900
Rosa, Silvina Mariana; Galvagno, Miguel Angel; Vélez, Carlos Guillermo; Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina; Springer Tokyo; Mycoscience; 52; 4; 7-2011; 242-252
1340-3540
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280900
identifier_str_mv Rosa, Silvina Mariana; Galvagno, Miguel Angel; Vélez, Carlos Guillermo; Adjusting culture conditions to isolate thraustochytrids from temperate and cold environments in southern Argentina; Springer Tokyo; Mycoscience; 52; 4; 7-2011; 242-252
1340-3540
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10267-010-0091-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Tokyo
publisher.none.fl_str_mv Springer Tokyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.115731

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