The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub

Autores
Golluscio, Rodolfo; Irueta, Regina; Cipriotti, Pablo Ariel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The comparison of 15N natural abundance (δ15N) between fixing and non-fixing reference species, is the most feasible method for nitrogen fixation studies in xeric shrubs. However, it assumes that both species use the same sources of soil N, the δ15N of such source is clearly different from zero, and the difference between the δ15N of both species is ≥ 5‰. On the other hand, the analysis of the difference between the δ15N of nodules and leaves (Δδ15Nn−l) does not require a reference species. To evaluate the effect of water availability on nitrogen fixation, we analyzed the variation of δ15N and Δδ15Nn−l in watered and unwatered plants of Adesmia volckmanni Phil. (Fabaceae) and Mulinum spinosum (Cav.) Pers. (Apiaceae), formerly proposed as non-fixing reference species. However, we found that M. spinosum was not valid as reference species to study N fixation in Adesmia volckmann because its δ15N was closer to zero than that of the presumably fixing species, suggesting they use different soil N sources. In addition, water availability did not affect biological fixation after watering because both leaf δ15N and Δδ15Nn−l of A. volckmanni did not change either as soil dried up along the growing season nor in response to watering. However, the presence of nodules itself, the δ15N closer to zero than in a former dry year, and the Δδ15Nn−l (+ 1.23‰) suggest that biological fixation could have occurred before watering, probably during early spring.
Fil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina
Fil: Irueta, Regina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina
Fil: Cipriotti, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Métodos Cuantitativos y Sistemas de Información; Argentina
Materia
Elusive Measurement
Nitrogen Fixation
Xeric Shrubs
Adesmia Volckmanni
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4174
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4174
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrubGolluscio, RodolfoIrueta, ReginaCipriotti, Pablo ArielElusive MeasurementNitrogen FixationXeric ShrubsAdesmia Volckmannihttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The comparison of 15N natural abundance (δ15N) between fixing and non-fixing reference species, is the most feasible method for nitrogen fixation studies in xeric shrubs. However, it assumes that both species use the same sources of soil N, the δ15N of such source is clearly different from zero, and the difference between the δ15N of both species is ≥ 5‰. On the other hand, the analysis of the difference between the δ15N of nodules and leaves (Δδ15Nn−l) does not require a reference species. To evaluate the effect of water availability on nitrogen fixation, we analyzed the variation of δ15N and Δδ15Nn−l in watered and unwatered plants of Adesmia volckmanni Phil. (Fabaceae) and Mulinum spinosum (Cav.) Pers. (Apiaceae), formerly proposed as non-fixing reference species. However, we found that M. spinosum was not valid as reference species to study N fixation in Adesmia volckmann because its δ15N was closer to zero than that of the presumably fixing species, suggesting they use different soil N sources. In addition, water availability did not affect biological fixation after watering because both leaf δ15N and Δδ15Nn−l of A. volckmanni did not change either as soil dried up along the growing season nor in response to watering. However, the presence of nodules itself, the δ15N closer to zero than in a former dry year, and the Δδ15Nn−l (+ 1.23‰) suggest that biological fixation could have occurred before watering, probably during early spring.Fil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; ArgentinaFil: Irueta, Regina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; ArgentinaFil: Cipriotti, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Métodos Cuantitativos y Sistemas de Información; ArgentinaElsevier2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4174Golluscio, Rodolfo; Irueta, Regina; Cipriotti, Pablo Ariel; The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub; Elsevier; Journal of Arid Environments; 111; 6-2014; 22-260140-1963enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0140196314001633info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2014.07.006info:eu-repo/semantics/altIdentifier/issn/0140-1963info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:06Zoai:ri.conicet.gov.ar:11336/4174instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:06.348CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
title The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
spellingShingle The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
Golluscio, Rodolfo
Elusive Measurement
Nitrogen Fixation
Xeric Shrubs
Adesmia Volckmanni
title_short The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
title_full The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
title_fullStr The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
title_full_unstemmed The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
title_sort The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub
dc.creator.none.fl_str_mv Golluscio, Rodolfo
Irueta, Regina
Cipriotti, Pablo Ariel
author Golluscio, Rodolfo
author_facet Golluscio, Rodolfo
Irueta, Regina
Cipriotti, Pablo Ariel
author_role author
author2 Irueta, Regina
Cipriotti, Pablo Ariel
author2_role author
author
dc.subject.none.fl_str_mv Elusive Measurement
Nitrogen Fixation
Xeric Shrubs
Adesmia Volckmanni
topic Elusive Measurement
Nitrogen Fixation
Xeric Shrubs
Adesmia Volckmanni
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The comparison of 15N natural abundance (δ15N) between fixing and non-fixing reference species, is the most feasible method for nitrogen fixation studies in xeric shrubs. However, it assumes that both species use the same sources of soil N, the δ15N of such source is clearly different from zero, and the difference between the δ15N of both species is ≥ 5‰. On the other hand, the analysis of the difference between the δ15N of nodules and leaves (Δδ15Nn−l) does not require a reference species. To evaluate the effect of water availability on nitrogen fixation, we analyzed the variation of δ15N and Δδ15Nn−l in watered and unwatered plants of Adesmia volckmanni Phil. (Fabaceae) and Mulinum spinosum (Cav.) Pers. (Apiaceae), formerly proposed as non-fixing reference species. However, we found that M. spinosum was not valid as reference species to study N fixation in Adesmia volckmann because its δ15N was closer to zero than that of the presumably fixing species, suggesting they use different soil N sources. In addition, water availability did not affect biological fixation after watering because both leaf δ15N and Δδ15Nn−l of A. volckmanni did not change either as soil dried up along the growing season nor in response to watering. However, the presence of nodules itself, the δ15N closer to zero than in a former dry year, and the Δδ15Nn−l (+ 1.23‰) suggest that biological fixation could have occurred before watering, probably during early spring.
Fil: Golluscio, Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina
Fil: Irueta, Regina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal; Argentina
Fil: Cipriotti, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Métodos Cuantitativos y Sistemas de Información; Argentina
description The comparison of 15N natural abundance (δ15N) between fixing and non-fixing reference species, is the most feasible method for nitrogen fixation studies in xeric shrubs. However, it assumes that both species use the same sources of soil N, the δ15N of such source is clearly different from zero, and the difference between the δ15N of both species is ≥ 5‰. On the other hand, the analysis of the difference between the δ15N of nodules and leaves (Δδ15Nn−l) does not require a reference species. To evaluate the effect of water availability on nitrogen fixation, we analyzed the variation of δ15N and Δδ15Nn−l in watered and unwatered plants of Adesmia volckmanni Phil. (Fabaceae) and Mulinum spinosum (Cav.) Pers. (Apiaceae), formerly proposed as non-fixing reference species. However, we found that M. spinosum was not valid as reference species to study N fixation in Adesmia volckmann because its δ15N was closer to zero than that of the presumably fixing species, suggesting they use different soil N sources. In addition, water availability did not affect biological fixation after watering because both leaf δ15N and Δδ15Nn−l of A. volckmanni did not change either as soil dried up along the growing season nor in response to watering. However, the presence of nodules itself, the δ15N closer to zero than in a former dry year, and the Δδ15Nn−l (+ 1.23‰) suggest that biological fixation could have occurred before watering, probably during early spring.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4174
Golluscio, Rodolfo; Irueta, Regina; Cipriotti, Pablo Ariel; The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub; Elsevier; Journal of Arid Environments; 111; 6-2014; 22-26
0140-1963
url http://hdl.handle.net/11336/4174
identifier_str_mv Golluscio, Rodolfo; Irueta, Regina; Cipriotti, Pablo Ariel; The elusive quantifi cation of nitrogen fi xation in xeric shrubs: The case of Adesmia volckmanni, a Patagonian leguminous shrub; Elsevier; Journal of Arid Environments; 111; 6-2014; 22-26
0140-1963
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0140196314001633
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jaridenv.2014.07.006
info:eu-repo/semantics/altIdentifier/issn/0140-1963
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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