POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Autores
Houbaert, Anaxi; Zhang, Cheng; Tiwari, Manish; Wang, Kun; Marcos Serrano, Alberto de; Savatin, Daniel V.; Urs, Mounashree J.; Zhiponova, Miroslava; Gudesblat, Gustavo Eduardo; Vanhoutte, Isabelle; Eeckhout, Dominique; Boeren, Sjef; Karimi, Mansour; Betti, Camilla; Jacobs, Thomas; Fenoll, Carmen; Mena, Montaña; de Vries, Sacco; De Jaeger, Geert; Russinova, Eugenia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.
Fil: Houbaert, Anaxi. University of Ghent; Bélgica
Fil: Zhang, Cheng. University of Ghent; Bélgica
Fil: Tiwari, Manish. University of Ghent; Bélgica
Fil: Wang, Kun. Wuhan University; China. University of Ghent; Bélgica
Fil: Marcos Serrano, Alberto de. Universidad de Castilla-La Mancha; España
Fil: Savatin, Daniel V.. University of Ghent; Bélgica
Fil: Urs, Mounashree J.. University of Ghent; Bélgica
Fil: Zhiponova, Miroslava. University of Sofia; Bulgaria. University of Ghent; Bélgica
Fil: Gudesblat, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. University of Ghent; Bélgica
Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica
Fil: Eeckhout, Dominique. University of Ghent; Bélgica
Fil: Boeren, Sjef. Wageningen University; Países Bajos
Fil: Karimi, Mansour. University of Ghent; Bélgica
Fil: Betti, Camilla. University of Ghent; Bélgica. Università di Perugia; Italia
Fil: Jacobs, Thomas. University of Ghent; Bélgica
Fil: Fenoll, Carmen. Universidad de Castilla-La Mancha; España
Fil: Mena, Montaña. Universidad de Castilla-La Mancha; España
Fil: de Vries, Sacco. Wageningen University; Países Bajos
Fil: De Jaeger, Geert. University of Ghent; Bélgica
Fil: Russinova, Eugenia. University of Ghent; Bélgica
Materia
Stomata
Brassinosteroid
GSK
Speechless
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96302
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96302
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling POLAR-guided signalling complex assembly and localization drive asymmetric cell divisionHoubaert, AnaxiZhang, ChengTiwari, ManishWang, KunMarcos Serrano, Alberto deSavatin, Daniel V.Urs, Mounashree J.Zhiponova, MiroslavaGudesblat, Gustavo EduardoVanhoutte, IsabelleEeckhout, DominiqueBoeren, SjefKarimi, MansourBetti, CamillaJacobs, ThomasFenoll, CarmenMena, Montañade Vries, SaccoDe Jaeger, GeertRussinova, EugeniaStomataBrassinosteroidGSKSpeechlesshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.Fil: Houbaert, Anaxi. University of Ghent; BélgicaFil: Zhang, Cheng. University of Ghent; BélgicaFil: Tiwari, Manish. University of Ghent; BélgicaFil: Wang, Kun. Wuhan University; China. University of Ghent; BélgicaFil: Marcos Serrano, Alberto de. Universidad de Castilla-La Mancha; EspañaFil: Savatin, Daniel V.. University of Ghent; BélgicaFil: Urs, Mounashree J.. University of Ghent; BélgicaFil: Zhiponova, Miroslava. University of Sofia; Bulgaria. University of Ghent; BélgicaFil: Gudesblat, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. University of Ghent; BélgicaFil: Vanhoutte, Isabelle. University of Ghent; BélgicaFil: Eeckhout, Dominique. University of Ghent; BélgicaFil: Boeren, Sjef. Wageningen University; Países BajosFil: Karimi, Mansour. University of Ghent; BélgicaFil: Betti, Camilla. University of Ghent; Bélgica. Università di Perugia; ItaliaFil: Jacobs, Thomas. University of Ghent; BélgicaFil: Fenoll, Carmen. Universidad de Castilla-La Mancha; EspañaFil: Mena, Montaña. Universidad de Castilla-La Mancha; EspañaFil: de Vries, Sacco. Wageningen University; Países BajosFil: De Jaeger, Geert. University of Ghent; BélgicaFil: Russinova, Eugenia. University of Ghent; BélgicaNature Publishing Group2018-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96302Houbaert, Anaxi; Zhang, Cheng; Tiwari, Manish; Wang, Kun; Marcos Serrano, Alberto de; et al.; POLAR-guided signalling complex assembly and localization drive asymmetric cell division; Nature Publishing Group; Nature; 563; 7732; 11-2018; 574-5780028-0836CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41586-018-0714-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41586-018-0714-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:41Zoai:ri.conicet.gov.ar:11336/96302instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:42.175CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title POLAR-guided signalling complex assembly and localization drive asymmetric cell division
spellingShingle POLAR-guided signalling complex assembly and localization drive asymmetric cell division
Houbaert, Anaxi
Stomata
Brassinosteroid
GSK
Speechless
title_short POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_full POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_fullStr POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_full_unstemmed POLAR-guided signalling complex assembly and localization drive asymmetric cell division
title_sort POLAR-guided signalling complex assembly and localization drive asymmetric cell division
dc.creator.none.fl_str_mv Houbaert, Anaxi
Zhang, Cheng
Tiwari, Manish
Wang, Kun
Marcos Serrano, Alberto de
Savatin, Daniel V.
Urs, Mounashree J.
Zhiponova, Miroslava
Gudesblat, Gustavo Eduardo
Vanhoutte, Isabelle
Eeckhout, Dominique
Boeren, Sjef
Karimi, Mansour
Betti, Camilla
Jacobs, Thomas
Fenoll, Carmen
Mena, Montaña
de Vries, Sacco
De Jaeger, Geert
Russinova, Eugenia
author Houbaert, Anaxi
author_facet Houbaert, Anaxi
Zhang, Cheng
Tiwari, Manish
Wang, Kun
Marcos Serrano, Alberto de
Savatin, Daniel V.
Urs, Mounashree J.
Zhiponova, Miroslava
Gudesblat, Gustavo Eduardo
Vanhoutte, Isabelle
Eeckhout, Dominique
Boeren, Sjef
Karimi, Mansour
Betti, Camilla
Jacobs, Thomas
Fenoll, Carmen
Mena, Montaña
de Vries, Sacco
De Jaeger, Geert
Russinova, Eugenia
author_role author
author2 Zhang, Cheng
Tiwari, Manish
Wang, Kun
Marcos Serrano, Alberto de
Savatin, Daniel V.
Urs, Mounashree J.
Zhiponova, Miroslava
Gudesblat, Gustavo Eduardo
Vanhoutte, Isabelle
Eeckhout, Dominique
Boeren, Sjef
Karimi, Mansour
Betti, Camilla
Jacobs, Thomas
Fenoll, Carmen
Mena, Montaña
de Vries, Sacco
De Jaeger, Geert
Russinova, Eugenia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Stomata
Brassinosteroid
GSK
Speechless
topic Stomata
Brassinosteroid
GSK
Speechless
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.
Fil: Houbaert, Anaxi. University of Ghent; Bélgica
Fil: Zhang, Cheng. University of Ghent; Bélgica
Fil: Tiwari, Manish. University of Ghent; Bélgica
Fil: Wang, Kun. Wuhan University; China. University of Ghent; Bélgica
Fil: Marcos Serrano, Alberto de. Universidad de Castilla-La Mancha; España
Fil: Savatin, Daniel V.. University of Ghent; Bélgica
Fil: Urs, Mounashree J.. University of Ghent; Bélgica
Fil: Zhiponova, Miroslava. University of Sofia; Bulgaria. University of Ghent; Bélgica
Fil: Gudesblat, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. University of Ghent; Bélgica
Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica
Fil: Eeckhout, Dominique. University of Ghent; Bélgica
Fil: Boeren, Sjef. Wageningen University; Países Bajos
Fil: Karimi, Mansour. University of Ghent; Bélgica
Fil: Betti, Camilla. University of Ghent; Bélgica. Università di Perugia; Italia
Fil: Jacobs, Thomas. University of Ghent; Bélgica
Fil: Fenoll, Carmen. Universidad de Castilla-La Mancha; España
Fil: Mena, Montaña. Universidad de Castilla-La Mancha; España
Fil: de Vries, Sacco. Wageningen University; Países Bajos
Fil: De Jaeger, Geert. University of Ghent; Bélgica
Fil: Russinova, Eugenia. University of Ghent; Bélgica
description Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.
publishDate 2018
dc.date.none.fl_str_mv 2018-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96302
Houbaert, Anaxi; Zhang, Cheng; Tiwari, Manish; Wang, Kun; Marcos Serrano, Alberto de; et al.; POLAR-guided signalling complex assembly and localization drive asymmetric cell division; Nature Publishing Group; Nature; 563; 7732; 11-2018; 574-578
0028-0836
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96302
identifier_str_mv Houbaert, Anaxi; Zhang, Cheng; Tiwari, Manish; Wang, Kun; Marcos Serrano, Alberto de; et al.; POLAR-guided signalling complex assembly and localization drive asymmetric cell division; Nature Publishing Group; Nature; 563; 7732; 11-2018; 574-578
0028-0836
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41586-018-0714-x
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41586-018-0714-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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