SPEECHLESS integrates brassinosteroid and stomata signalling pathways

Autores
Gudesblat, Gustavo Eduardo; Schneider Pizon, Joanna; Betti, Camilla; Mayerhofer, Juliane; Vanhoutte, Isabelle; Dongen, Walter van; Boeren, Sjef; Zhiponova, Miroslava; de Vries, Sacco; Jonak, Claudia; Russinova, Eugenia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood. In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation. Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
Fil: Gudesblat, Gustavo Eduardo. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina
Fil: Schneider Pizon, Joanna. University of Ghent; Bélgica
Fil: Betti, Camilla. University of Ghent; Bélgica
Fil: Mayerhofer, Juliane. Austrian Academy of Sciences; Austria
Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica
Fil: Dongen, Walter van. Wageningen University; Países Bajos
Fil: Boeren, Sjef. Wageningen University; Países Bajos
Fil: Zhiponova, Miroslava. University of Ghent; Bélgica
Fil: de Vries, Sacco. Wageningen University; Países Bajos
Fil: Jonak, Claudia. Austrian Academy of Sciences; Austria
Fil: Russinova, Eugenia. University of Ghent; Bélgica. University of Sofia; Bulgaria
Materia
Brasinossinosteroids
Stomata
Gsk3
Speechless
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16902
Acceder
id CONICETDig_2534668c35480e702e96345842801bb9
oai_identifier_str oai:ri.conicet.gov.ar:11336/16902
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling SPEECHLESS integrates brassinosteroid and stomata signalling pathwaysGudesblat, Gustavo EduardoSchneider Pizon, JoannaBetti, CamillaMayerhofer, JulianeVanhoutte, IsabelleDongen, Walter vanBoeren, SjefZhiponova, Miroslavade Vries, SaccoJonak, ClaudiaRussinova, EugeniaBrasinossinosteroidsStomataGsk3Speechlesshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood. In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation. Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.Fil: Gudesblat, Gustavo Eduardo. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; ArgentinaFil: Schneider Pizon, Joanna. University of Ghent; BélgicaFil: Betti, Camilla. University of Ghent; BélgicaFil: Mayerhofer, Juliane. Austrian Academy of Sciences; AustriaFil: Vanhoutte, Isabelle. University of Ghent; BélgicaFil: Dongen, Walter van. Wageningen University; Países BajosFil: Boeren, Sjef. Wageningen University; Países BajosFil: Zhiponova, Miroslava. University of Ghent; BélgicaFil: de Vries, Sacco. Wageningen University; Países BajosFil: Jonak, Claudia. Austrian Academy of Sciences; AustriaFil: Russinova, Eugenia. University of Ghent; Bélgica. University of Sofia; BulgariaNature Publishing Group2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16902Gudesblat, Gustavo Eduardo; Schneider Pizon, Joanna; Betti, Camilla; Mayerhofer, Juliane; Vanhoutte, Isabelle; et al.; SPEECHLESS integrates brassinosteroid and stomata signalling pathways; Nature Publishing Group; Nature Cell Biology.; 14; 5; 5-2012; 548-5541465-73921476-4679enginfo:eu-repo/semantics/altIdentifier/doi/10.1038/ncb2471info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/ncb/journal/v14/n5/full/ncb2471.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:37Zoai:ri.conicet.gov.ar:11336/16902instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:37.854CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SPEECHLESS integrates brassinosteroid and stomata signalling pathways
title SPEECHLESS integrates brassinosteroid and stomata signalling pathways
spellingShingle SPEECHLESS integrates brassinosteroid and stomata signalling pathways
Gudesblat, Gustavo Eduardo
Brasinossinosteroids
Stomata
Gsk3
Speechless
title_short SPEECHLESS integrates brassinosteroid and stomata signalling pathways
title_full SPEECHLESS integrates brassinosteroid and stomata signalling pathways
title_fullStr SPEECHLESS integrates brassinosteroid and stomata signalling pathways
title_full_unstemmed SPEECHLESS integrates brassinosteroid and stomata signalling pathways
title_sort SPEECHLESS integrates brassinosteroid and stomata signalling pathways
dc.creator.none.fl_str_mv Gudesblat, Gustavo Eduardo
Schneider Pizon, Joanna
Betti, Camilla
Mayerhofer, Juliane
Vanhoutte, Isabelle
Dongen, Walter van
Boeren, Sjef
Zhiponova, Miroslava
de Vries, Sacco
Jonak, Claudia
Russinova, Eugenia
author Gudesblat, Gustavo Eduardo
author_facet Gudesblat, Gustavo Eduardo
Schneider Pizon, Joanna
Betti, Camilla
Mayerhofer, Juliane
Vanhoutte, Isabelle
Dongen, Walter van
Boeren, Sjef
Zhiponova, Miroslava
de Vries, Sacco
Jonak, Claudia
Russinova, Eugenia
author_role author
author2 Schneider Pizon, Joanna
Betti, Camilla
Mayerhofer, Juliane
Vanhoutte, Isabelle
Dongen, Walter van
Boeren, Sjef
Zhiponova, Miroslava
de Vries, Sacco
Jonak, Claudia
Russinova, Eugenia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Brasinossinosteroids
Stomata
Gsk3
Speechless
topic Brasinossinosteroids
Stomata
Gsk3
Speechless
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood. In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation. Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
Fil: Gudesblat, Gustavo Eduardo. University of Ghent; Bélgica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. Cesar Milstein". Fundación Pablo Cassara. Instituto de Ciencia y Tecnología ; Argentina
Fil: Schneider Pizon, Joanna. University of Ghent; Bélgica
Fil: Betti, Camilla. University of Ghent; Bélgica
Fil: Mayerhofer, Juliane. Austrian Academy of Sciences; Austria
Fil: Vanhoutte, Isabelle. University of Ghent; Bélgica
Fil: Dongen, Walter van. Wageningen University; Países Bajos
Fil: Boeren, Sjef. Wageningen University; Países Bajos
Fil: Zhiponova, Miroslava. University of Ghent; Bélgica
Fil: de Vries, Sacco. Wageningen University; Países Bajos
Fil: Jonak, Claudia. Austrian Academy of Sciences; Austria
Fil: Russinova, Eugenia. University of Ghent; Bélgica. University of Sofia; Bulgaria
description Stomatal formation is regulated by multiple developmental and environmental signals, but how these signals are integrated to control this process is not fully understood. In Arabidopsis thaliana, the basic helix-loop-helix transcription factor SPEECHLESS (SPCH) regulates the entry, amplifying and spacing divisions that occur during stomatal lineage development. SPCH activity is negatively regulated by mitogen-activated protein kinase (MAPK)-mediated phosphorylation. Here, we show that in addition to MAPKs, SPCH activity is also modulated by brassinosteroid (BR) signalling. The GSK3/SHAGGY-like kinase BIN2 (BR INSENSITIVE2) phosphorylates residues overlapping those targeted by the MAPKs, as well as four residues in the amino-terminal region of the protein outside the MAPK target domain. These phosphorylation events antagonize SPCH activity and limit epidermal cell proliferation. Conversely, inhibition of BIN2 activity in vivo stabilizes SPCH and triggers excessive stomatal and non-stomatal cell formation. We demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16902
Gudesblat, Gustavo Eduardo; Schneider Pizon, Joanna; Betti, Camilla; Mayerhofer, Juliane; Vanhoutte, Isabelle; et al.; SPEECHLESS integrates brassinosteroid and stomata signalling pathways; Nature Publishing Group; Nature Cell Biology.; 14; 5; 5-2012; 548-554
1465-7392
1476-4679
url http://hdl.handle.net/11336/16902
identifier_str_mv Gudesblat, Gustavo Eduardo; Schneider Pizon, Joanna; Betti, Camilla; Mayerhofer, Juliane; Vanhoutte, Isabelle; et al.; SPEECHLESS integrates brassinosteroid and stomata signalling pathways; Nature Publishing Group; Nature Cell Biology.; 14; 5; 5-2012; 548-554
1465-7392
1476-4679
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1038/ncb2471
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/ncb/journal/v14/n5/full/ncb2471.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269966997389312
score 13.13397

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