New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production

Autores
Racca, Sofia; Leonardi, Rodrigo Jorge; Comelli, Raul Nicolas
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bioethanol is a sustainable, low-impact energy source with the potential to reduce or even replace fossil fuel consumption. Second-generation (2G) bioethanol exploits lignocellulosic agro-industrial residues, contributing to circular economy strategies by valorizing these waste streams. However, conventional Saccharomyces cerevisiae strains are unable to efficiently metabolize the pentose sugars abundant in lignocellulose, prompting growing interest in non-conventional yeasts such as Spathaspora passalidarum. This species, recognized for its innate ability to assimilate pentoses, remains underexplored, particularly regarding its metabolic performance in mixed sugar environments containing hexoses, pentoses, and disaccharides. Our results demonstrate that S. passalidarum’s xylose metabolism is strongly inhibited by pulses of hexoses such as glucose, galactose, and mannose, as well as by the disaccharide maltose. Notably, inhibition was also triggered by the non-metabolizable glucose analog 2-deoxyglucose (2DG), indicating that the regulatory signal originates during the early stages of glucose uptake into the cytosol rather than from downstream glycolytic pathways. In contrast, xylose metabolism was prioritized over fructose and sucrose. Furthermore, S. passalidarum was able to metabolize arabinose and glycerol, although these pathways favored biomass production through oxygen-dependent processes. Arabinose could be co-metabolized with xylose, but its assimilation was markedly suppressed in the presence of glucose. Collectively, these findings provide new insights into the metabolic regulation of S. passalidarum and highlight its potential role in the design of robust strategies for 2G bioethanol production.
Fil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Comelli, Raul Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
Materia
BIOETHANOL
NON-CONVENTIONAL YEAST
SPATHASPORA PASSALIDARUM
YEAST METABOLISM
FERMENTATION
SUGAR METABOLISM
XYLOSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278925
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol productionRacca, SofiaLeonardi, Rodrigo JorgeComelli, Raul NicolasBIOETHANOLNON-CONVENTIONAL YEASTSPATHASPORA PASSALIDARUMYEAST METABOLISMFERMENTATIONSUGAR METABOLISMXYLOSEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Bioethanol is a sustainable, low-impact energy source with the potential to reduce or even replace fossil fuel consumption. Second-generation (2G) bioethanol exploits lignocellulosic agro-industrial residues, contributing to circular economy strategies by valorizing these waste streams. However, conventional Saccharomyces cerevisiae strains are unable to efficiently metabolize the pentose sugars abundant in lignocellulose, prompting growing interest in non-conventional yeasts such as Spathaspora passalidarum. This species, recognized for its innate ability to assimilate pentoses, remains underexplored, particularly regarding its metabolic performance in mixed sugar environments containing hexoses, pentoses, and disaccharides. Our results demonstrate that S. passalidarum’s xylose metabolism is strongly inhibited by pulses of hexoses such as glucose, galactose, and mannose, as well as by the disaccharide maltose. Notably, inhibition was also triggered by the non-metabolizable glucose analog 2-deoxyglucose (2DG), indicating that the regulatory signal originates during the early stages of glucose uptake into the cytosol rather than from downstream glycolytic pathways. In contrast, xylose metabolism was prioritized over fructose and sucrose. Furthermore, S. passalidarum was able to metabolize arabinose and glycerol, although these pathways favored biomass production through oxygen-dependent processes. Arabinose could be co-metabolized with xylose, but its assimilation was markedly suppressed in the presence of glucose. Collectively, these findings provide new insights into the metabolic regulation of S. passalidarum and highlight its potential role in the design of robust strategies for 2G bioethanol production.Fil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Comelli, Raul Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; ArgentinaFrontiers Media2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278925Racca, Sofia; Leonardi, Rodrigo Jorge; Comelli, Raul Nicolas; New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production; Frontiers Media; Frontiers in Fungal Biology; 6; 9-2025; 1-162673-6128CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/ffunb.2025.1657121/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2025.1657121info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-14T12:53:31Zoai:ri.conicet.gov.ar:11336/278925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-14 12:53:31.641CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
title New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
spellingShingle New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
Racca, Sofia
BIOETHANOL
NON-CONVENTIONAL YEAST
SPATHASPORA PASSALIDARUM
YEAST METABOLISM
FERMENTATION
SUGAR METABOLISM
XYLOSE
title_short New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
title_full New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
title_fullStr New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
title_full_unstemmed New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
title_sort New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production
dc.creator.none.fl_str_mv Racca, Sofia
Leonardi, Rodrigo Jorge
Comelli, Raul Nicolas
author Racca, Sofia
author_facet Racca, Sofia
Leonardi, Rodrigo Jorge
Comelli, Raul Nicolas
author_role author
author2 Leonardi, Rodrigo Jorge
Comelli, Raul Nicolas
author2_role author
author
dc.subject.none.fl_str_mv BIOETHANOL
NON-CONVENTIONAL YEAST
SPATHASPORA PASSALIDARUM
YEAST METABOLISM
FERMENTATION
SUGAR METABOLISM
XYLOSE
topic BIOETHANOL
NON-CONVENTIONAL YEAST
SPATHASPORA PASSALIDARUM
YEAST METABOLISM
FERMENTATION
SUGAR METABOLISM
XYLOSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bioethanol is a sustainable, low-impact energy source with the potential to reduce or even replace fossil fuel consumption. Second-generation (2G) bioethanol exploits lignocellulosic agro-industrial residues, contributing to circular economy strategies by valorizing these waste streams. However, conventional Saccharomyces cerevisiae strains are unable to efficiently metabolize the pentose sugars abundant in lignocellulose, prompting growing interest in non-conventional yeasts such as Spathaspora passalidarum. This species, recognized for its innate ability to assimilate pentoses, remains underexplored, particularly regarding its metabolic performance in mixed sugar environments containing hexoses, pentoses, and disaccharides. Our results demonstrate that S. passalidarum’s xylose metabolism is strongly inhibited by pulses of hexoses such as glucose, galactose, and mannose, as well as by the disaccharide maltose. Notably, inhibition was also triggered by the non-metabolizable glucose analog 2-deoxyglucose (2DG), indicating that the regulatory signal originates during the early stages of glucose uptake into the cytosol rather than from downstream glycolytic pathways. In contrast, xylose metabolism was prioritized over fructose and sucrose. Furthermore, S. passalidarum was able to metabolize arabinose and glycerol, although these pathways favored biomass production through oxygen-dependent processes. Arabinose could be co-metabolized with xylose, but its assimilation was markedly suppressed in the presence of glucose. Collectively, these findings provide new insights into the metabolic regulation of S. passalidarum and highlight its potential role in the design of robust strategies for 2G bioethanol production.
Fil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Leonardi, Rodrigo Jorge. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Comelli, Raul Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hidricas. Departamento de Medio Ambiente; Argentina
description Bioethanol is a sustainable, low-impact energy source with the potential to reduce or even replace fossil fuel consumption. Second-generation (2G) bioethanol exploits lignocellulosic agro-industrial residues, contributing to circular economy strategies by valorizing these waste streams. However, conventional Saccharomyces cerevisiae strains are unable to efficiently metabolize the pentose sugars abundant in lignocellulose, prompting growing interest in non-conventional yeasts such as Spathaspora passalidarum. This species, recognized for its innate ability to assimilate pentoses, remains underexplored, particularly regarding its metabolic performance in mixed sugar environments containing hexoses, pentoses, and disaccharides. Our results demonstrate that S. passalidarum’s xylose metabolism is strongly inhibited by pulses of hexoses such as glucose, galactose, and mannose, as well as by the disaccharide maltose. Notably, inhibition was also triggered by the non-metabolizable glucose analog 2-deoxyglucose (2DG), indicating that the regulatory signal originates during the early stages of glucose uptake into the cytosol rather than from downstream glycolytic pathways. In contrast, xylose metabolism was prioritized over fructose and sucrose. Furthermore, S. passalidarum was able to metabolize arabinose and glycerol, although these pathways favored biomass production through oxygen-dependent processes. Arabinose could be co-metabolized with xylose, but its assimilation was markedly suppressed in the presence of glucose. Collectively, these findings provide new insights into the metabolic regulation of S. passalidarum and highlight its potential role in the design of robust strategies for 2G bioethanol production.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278925
Racca, Sofia; Leonardi, Rodrigo Jorge; Comelli, Raul Nicolas; New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production; Frontiers Media; Frontiers in Fungal Biology; 6; 9-2025; 1-16
2673-6128
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278925
identifier_str_mv Racca, Sofia; Leonardi, Rodrigo Jorge; Comelli, Raul Nicolas; New insights into carbon metabolism in Spathaspora passalidarum for second-generation ethanol production; Frontiers Media; Frontiers in Fungal Biology; 6; 9-2025; 1-16
2673-6128
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/ffunb.2025.1657121/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/ffunb.2025.1657121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.113929

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