Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses

Autores
Rodriguez Prado, Macarena; Muruaga, María Laura; Perotti, Nora Ines; Abate, Carlos Mauricio
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.
Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
VI Congreso Argentino de Microbiología General
Carlos Paz
Argentina
Sociedad Argentina de Microbiología General
Materia
ETHANOL
YEAST
BIOETHANOL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195002
Acceder
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spelling Selection and identification of ethanol hiperproducer yeasts from sugar cane molassesRodriguez Prado, MacarenaMuruaga, María LauraPerotti, Nora InesAbate, Carlos MauricioETHANOLYEASTBIOETHANOLhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaVI Congreso Argentino de Microbiología GeneralCarlos PazArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195002Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2009.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:31:17Zoai:ri.conicet.gov.ar:11336/195002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:31:18.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
title Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
spellingShingle Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
Rodriguez Prado, Macarena
ETHANOL
YEAST
BIOETHANOL
title_short Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
title_full Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
title_fullStr Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
title_full_unstemmed Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
title_sort Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
dc.creator.none.fl_str_mv Rodriguez Prado, Macarena
Muruaga, María Laura
Perotti, Nora Ines
Abate, Carlos Mauricio
author Rodriguez Prado, Macarena
author_facet Rodriguez Prado, Macarena
Muruaga, María Laura
Perotti, Nora Ines
Abate, Carlos Mauricio
author_role author
author2 Muruaga, María Laura
Perotti, Nora Ines
Abate, Carlos Mauricio
author2_role author
author
author
dc.subject.none.fl_str_mv ETHANOL
YEAST
BIOETHANOL
topic ETHANOL
YEAST
BIOETHANOL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.
Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
VI Congreso Argentino de Microbiología General
Carlos Paz
Argentina
Sociedad Argentina de Microbiología General
description The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195002
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195002
identifier_str_mv Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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