Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses
- Autores
- Rodriguez Prado, Macarena; Muruaga, María Laura; Perotti, Nora Ines; Abate, Carlos Mauricio
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.
Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
VI Congreso Argentino de Microbiología General
Carlos Paz
Argentina
Sociedad Argentina de Microbiología General - Materia
-
ETHANOL
YEAST
BIOETHANOL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/195002
Ver los metadatos del registro completo
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Selection and identification of ethanol hiperproducer yeasts from sugar cane molassesRodriguez Prado, MacarenaMuruaga, María LauraPerotti, Nora InesAbate, Carlos MauricioETHANOLYEASTBIOETHANOLhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production.Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaVI Congreso Argentino de Microbiología GeneralCarlos PazArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195002Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2009.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:24Zoai:ri.conicet.gov.ar:11336/195002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:24.872CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
title |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
spellingShingle |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses Rodriguez Prado, Macarena ETHANOL YEAST BIOETHANOL |
title_short |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
title_full |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
title_fullStr |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
title_full_unstemmed |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
title_sort |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses |
dc.creator.none.fl_str_mv |
Rodriguez Prado, Macarena Muruaga, María Laura Perotti, Nora Ines Abate, Carlos Mauricio |
author |
Rodriguez Prado, Macarena |
author_facet |
Rodriguez Prado, Macarena Muruaga, María Laura Perotti, Nora Ines Abate, Carlos Mauricio |
author_role |
author |
author2 |
Muruaga, María Laura Perotti, Nora Ines Abate, Carlos Mauricio |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
ETHANOL YEAST BIOETHANOL |
topic |
ETHANOL YEAST BIOETHANOL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.9 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production. Fil: Rodriguez Prado, Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Muruaga, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina VI Congreso Argentino de Microbiología General Carlos Paz Argentina Sociedad Argentina de Microbiología General |
description |
The decrease of the petroliferous stock and gas in the world, coupled with environmental pollution and global warming, caused mainly by the excessive use of such fuels has generated the need for further studies to become feasible to use new energy sources. The bioethanol consumption produce lower evaporative emissions due to its higher octane concentration and vapor pressure in consequence the pollution levels are much lower than the observed with fossil fuels. The entry into force of Law 26,093 of biofuels in Argentina from 2010 will mean an opportunity for the sugar sector to expand ethanol production to supply 5% of it to all the naphthas. In that regard, Tucumán has a privileged position since its large capacity of ethanol production associated to sugar mills. Our work proposes a microbiological approach to use fermentative microorganisms with high tolerance to alcohol in order to increase ethanol concentration of 11% that is currently obtained in the factories by the alcoholic fermentation of molasses. To take up this, isolation and identification of ethanol hyper-producer strains of yeasts from sugar cane molasses was addressed. Molasses samples were taken from different sugar factories of Tucuman and used to inoculate YPS and YPD media with antibiotics. The microorganisms were inoculated in YPS medium with 50g/L sucrose, incubated at 30ºC with agitation. The fermentations assays were carried out in Erlenmeyers flasks with 200 ml of YPS with 250 g/L of sucrose and incubated at 30 ºC without aeration. Total and direct reducing sugars, biomass and ethanol concentration were determined. Three isolates were selected by your high ethanol production and named as A2, A10 and A11, which produced 11.74, 12.81 and 13.20% of ethanol, respectively. The isolates were identified by sequence analysis of their ARNr 28S intergenic spacer sequences, which allowed assigning identities of 99 and 100% to Saccharomyces cerevisiae. Promising results obtained with isolates A10 and A11 justify further studies leading to an optimization of bioethanol production. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Congreso Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/195002 Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/195002 |
identifier_str_mv |
Selection and identification of ethanol hiperproducer yeasts from sugar cane molasses; VI Congreso Argentino de Microbiología General; Carlos Paz; Argentina; 2009; 112-112 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2009.pdf |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Sociedad Argentina de Microbiología General |
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Sociedad Argentina de Microbiología General |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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