Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions

Autores
Cunha Bolzico, Bruna; Racca, Sofia; Khawam, Jorge Nabil; Leonardi, Rodrigo Jorge; Tomassi, Ariel Hernán; Benzzo, María Teresita; Comelli, Raul Nicolas
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
d-Xylose is a metabolizable carbon source for several non-Saccharomyces species, but not for native strains of S. cerevisiae. For the potential application of xylose-assimilating yeasts in biotechnological processes, a deeper understanding of pentose catabolism is needed. This work aimed to investigate the traits behind xylose utilization in diverse yeast species. The performance of 9 selected xylose-metabolizing yeast strains was evaluated and compared across 3 oxygenation conditions. Oxygenation diversely impacted growth, xylose consumption, and product accumulation. Xylose utilization by ethanol-producing species such as Spathaspora passalidarum and Scheffersomyces stipitis was less affected by oxygen restriction compared with other xylitol-accumulating species such as Meyerozyma guilliermondii, Naganishia liquefaciens, and Yamadazyma sp., for which increased aeration stimulated xylose assimilation considerably. Spathaspora passalidarum exhibited superior conversion of xylose to ethanol and showed the fastest growth and xylose consumption in all 3 conditions. By performing assays under identical conditions for all selected yeasts, we minimize bias in comparisons, providing valuable insight into xylose metabolism and facilitating the development of robust bioprocesses.
Fil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Khawam, Jorge Nabil. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Leonardi, Rodrigo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Tomassi, Ariel Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
BIOETHANOL
XYLOSE
NON-CONVENTIONAL YEASTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256132
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditionsCunha Bolzico, BrunaRacca, SofiaKhawam, Jorge NabilLeonardi, Rodrigo JorgeTomassi, Ariel HernánBenzzo, María TeresitaComelli, Raul NicolasBIOETHANOLXYLOSENON-CONVENTIONAL YEASTShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2d-Xylose is a metabolizable carbon source for several non-Saccharomyces species, but not for native strains of S. cerevisiae. For the potential application of xylose-assimilating yeasts in biotechnological processes, a deeper understanding of pentose catabolism is needed. This work aimed to investigate the traits behind xylose utilization in diverse yeast species. The performance of 9 selected xylose-metabolizing yeast strains was evaluated and compared across 3 oxygenation conditions. Oxygenation diversely impacted growth, xylose consumption, and product accumulation. Xylose utilization by ethanol-producing species such as Spathaspora passalidarum and Scheffersomyces stipitis was less affected by oxygen restriction compared with other xylitol-accumulating species such as Meyerozyma guilliermondii, Naganishia liquefaciens, and Yamadazyma sp., for which increased aeration stimulated xylose assimilation considerably. Spathaspora passalidarum exhibited superior conversion of xylose to ethanol and showed the fastest growth and xylose consumption in all 3 conditions. By performing assays under identical conditions for all selected yeasts, we minimize bias in comparisons, providing valuable insight into xylose metabolism and facilitating the development of robust bioprocesses.Fil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Khawam, Jorge Nabil. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Leonardi, Rodrigo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Tomassi, Ariel Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; ArgentinaFil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaOxford University Press2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256132Cunha Bolzico, Bruna; Racca, Sofia; Khawam, Jorge Nabil; Leonardi, Rodrigo Jorge; Tomassi, Ariel Hernán; et al.; Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions; Oxford University Press; Journal of Industrial Microbiology & Biotechnology; 51; 8-2024; 1-141367-5435CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jimb/article/doi/10.1093/jimb/kuae023/7700697info:eu-repo/semantics/altIdentifier/doi/10.1093/jimb/kuae023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:18Zoai:ri.conicet.gov.ar:11336/256132instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:19.146CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
title Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
spellingShingle Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
Cunha Bolzico, Bruna
BIOETHANOL
XYLOSE
NON-CONVENTIONAL YEASTS
title_short Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
title_full Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
title_fullStr Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
title_full_unstemmed Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
title_sort Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions
dc.creator.none.fl_str_mv Cunha Bolzico, Bruna
Racca, Sofia
Khawam, Jorge Nabil
Leonardi, Rodrigo Jorge
Tomassi, Ariel Hernán
Benzzo, María Teresita
Comelli, Raul Nicolas
author Cunha Bolzico, Bruna
author_facet Cunha Bolzico, Bruna
Racca, Sofia
Khawam, Jorge Nabil
Leonardi, Rodrigo Jorge
Tomassi, Ariel Hernán
Benzzo, María Teresita
Comelli, Raul Nicolas
author_role author
author2 Racca, Sofia
Khawam, Jorge Nabil
Leonardi, Rodrigo Jorge
Tomassi, Ariel Hernán
Benzzo, María Teresita
Comelli, Raul Nicolas
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOETHANOL
XYLOSE
NON-CONVENTIONAL YEASTS
topic BIOETHANOL
XYLOSE
NON-CONVENTIONAL YEASTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv d-Xylose is a metabolizable carbon source for several non-Saccharomyces species, but not for native strains of S. cerevisiae. For the potential application of xylose-assimilating yeasts in biotechnological processes, a deeper understanding of pentose catabolism is needed. This work aimed to investigate the traits behind xylose utilization in diverse yeast species. The performance of 9 selected xylose-metabolizing yeast strains was evaluated and compared across 3 oxygenation conditions. Oxygenation diversely impacted growth, xylose consumption, and product accumulation. Xylose utilization by ethanol-producing species such as Spathaspora passalidarum and Scheffersomyces stipitis was less affected by oxygen restriction compared with other xylitol-accumulating species such as Meyerozyma guilliermondii, Naganishia liquefaciens, and Yamadazyma sp., for which increased aeration stimulated xylose assimilation considerably. Spathaspora passalidarum exhibited superior conversion of xylose to ethanol and showed the fastest growth and xylose consumption in all 3 conditions. By performing assays under identical conditions for all selected yeasts, we minimize bias in comparisons, providing valuable insight into xylose metabolism and facilitating the development of robust bioprocesses.
Fil: Cunha Bolzico, Bruna. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Racca, Sofia. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Khawam, Jorge Nabil. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Leonardi, Rodrigo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Tomassi, Ariel Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Benzzo, María Teresita. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina
Fil: Comelli, Raul Nicolas. Universidad Nacional del Litoral. Facultad de Ingeniería y Ciencias Hídricas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description d-Xylose is a metabolizable carbon source for several non-Saccharomyces species, but not for native strains of S. cerevisiae. For the potential application of xylose-assimilating yeasts in biotechnological processes, a deeper understanding of pentose catabolism is needed. This work aimed to investigate the traits behind xylose utilization in diverse yeast species. The performance of 9 selected xylose-metabolizing yeast strains was evaluated and compared across 3 oxygenation conditions. Oxygenation diversely impacted growth, xylose consumption, and product accumulation. Xylose utilization by ethanol-producing species such as Spathaspora passalidarum and Scheffersomyces stipitis was less affected by oxygen restriction compared with other xylitol-accumulating species such as Meyerozyma guilliermondii, Naganishia liquefaciens, and Yamadazyma sp., for which increased aeration stimulated xylose assimilation considerably. Spathaspora passalidarum exhibited superior conversion of xylose to ethanol and showed the fastest growth and xylose consumption in all 3 conditions. By performing assays under identical conditions for all selected yeasts, we minimize bias in comparisons, providing valuable insight into xylose metabolism and facilitating the development of robust bioprocesses.
publishDate 2024
dc.date.none.fl_str_mv 2024-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256132
Cunha Bolzico, Bruna; Racca, Sofia; Khawam, Jorge Nabil; Leonardi, Rodrigo Jorge; Tomassi, Ariel Hernán; et al.; Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions; Oxford University Press; Journal of Industrial Microbiology & Biotechnology; 51; 8-2024; 1-14
1367-5435
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256132
identifier_str_mv Cunha Bolzico, Bruna; Racca, Sofia; Khawam, Jorge Nabil; Leonardi, Rodrigo Jorge; Tomassi, Ariel Hernán; et al.; Exploring xylose metabolism in non-conventional yeasts: kinetic characterization and product accumulation under different aeration conditions; Oxford University Press; Journal of Industrial Microbiology & Biotechnology; 51; 8-2024; 1-14
1367-5435
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jimb/article/doi/10.1093/jimb/kuae023/7700697
info:eu-repo/semantics/altIdentifier/doi/10.1093/jimb/kuae023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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