Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water

Autores
Curvetto, Nestor Raul; Figlas, Norma Débora; Brandolin, Adriana; Saidman, Silvana Beatriz; Rueda, Elsa Haydee; Ferreira, María Luján
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present work analyzes the potential use of white-rot fungi (WRF) and hematin for phenol and aniline polymerization, as a low-cost alternative to horseradish peroxidase (HRPC). The objective is to evaluate the capability of these catalysts to produce tailor-made aniline as well as to eliminate phenols by precipitation from aqueous solution. 4-Aminoantypirine (4AAP) was used to test phenoxide formation by crude protein preparations of white-rot fungi at selected conditions. The crude extracts of Pleurotus sajor-caju (PSC) were selected because of the promising values obtained for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the presence of polystyrene sulphonated (PES), with high aniline conversions at 45 °C. For the case of insoluble polyphenol production, the PSC-derived enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversion was obtained when using PSC enzymes at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline (PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Brandolin, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Peroxidases
Polyaniline
Polyphenol
White-Rot Fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37801
Acceder
id CONICETDig_c6ff773dbfe3bf0e97b78f80ae31ab5a
oai_identifier_str oai:ri.conicet.gov.ar:11336/37801
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in waterCurvetto, Nestor RaulFiglas, Norma DéboraBrandolin, AdrianaSaidman, Silvana BeatrizRueda, Elsa HaydeeFerreira, María LujánPeroxidasesPolyanilinePolyphenolWhite-Rot Fungihttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The present work analyzes the potential use of white-rot fungi (WRF) and hematin for phenol and aniline polymerization, as a low-cost alternative to horseradish peroxidase (HRPC). The objective is to evaluate the capability of these catalysts to produce tailor-made aniline as well as to eliminate phenols by precipitation from aqueous solution. 4-Aminoantypirine (4AAP) was used to test phenoxide formation by crude protein preparations of white-rot fungi at selected conditions. The crude extracts of Pleurotus sajor-caju (PSC) were selected because of the promising values obtained for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the presence of polystyrene sulphonated (PES), with high aniline conversions at 45 °C. For the case of insoluble polyphenol production, the PSC-derived enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversion was obtained when using PSC enzymes at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline (PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Brandolin, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rueda, Elsa Haydee. Universidad Nacional del Sur; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaElsevier Science Sa2006-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37801Curvetto, Nestor Raul; Figlas, Norma Débora; Brandolin, Adriana; Saidman, Silvana Beatriz; Rueda, Elsa Haydee; et al.; Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water; Elsevier Science Sa; Biochemical Engineering Journal; 29; 3; 4-2006; 191-2031369-703XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2005.11.016info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X05003761info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:32Zoai:ri.conicet.gov.ar:11336/37801instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:33.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
title Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
spellingShingle Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
Curvetto, Nestor Raul
Peroxidases
Polyaniline
Polyphenol
White-Rot Fungi
title_short Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
title_full Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
title_fullStr Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
title_full_unstemmed Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
title_sort Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water
dc.creator.none.fl_str_mv Curvetto, Nestor Raul
Figlas, Norma Débora
Brandolin, Adriana
Saidman, Silvana Beatriz
Rueda, Elsa Haydee
Ferreira, María Luján
author Curvetto, Nestor Raul
author_facet Curvetto, Nestor Raul
Figlas, Norma Débora
Brandolin, Adriana
Saidman, Silvana Beatriz
Rueda, Elsa Haydee
Ferreira, María Luján
author_role author
author2 Figlas, Norma Débora
Brandolin, Adriana
Saidman, Silvana Beatriz
Rueda, Elsa Haydee
Ferreira, María Luján
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Peroxidases
Polyaniline
Polyphenol
White-Rot Fungi
topic Peroxidases
Polyaniline
Polyphenol
White-Rot Fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The present work analyzes the potential use of white-rot fungi (WRF) and hematin for phenol and aniline polymerization, as a low-cost alternative to horseradish peroxidase (HRPC). The objective is to evaluate the capability of these catalysts to produce tailor-made aniline as well as to eliminate phenols by precipitation from aqueous solution. 4-Aminoantypirine (4AAP) was used to test phenoxide formation by crude protein preparations of white-rot fungi at selected conditions. The crude extracts of Pleurotus sajor-caju (PSC) were selected because of the promising values obtained for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the presence of polystyrene sulphonated (PES), with high aniline conversions at 45 °C. For the case of insoluble polyphenol production, the PSC-derived enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversion was obtained when using PSC enzymes at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline (PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Brandolin, Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Saidman, Silvana Beatriz. Universidad Nacional del Sur; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rueda, Elsa Haydee. Universidad Nacional del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description The present work analyzes the potential use of white-rot fungi (WRF) and hematin for phenol and aniline polymerization, as a low-cost alternative to horseradish peroxidase (HRPC). The objective is to evaluate the capability of these catalysts to produce tailor-made aniline as well as to eliminate phenols by precipitation from aqueous solution. 4-Aminoantypirine (4AAP) was used to test phenoxide formation by crude protein preparations of white-rot fungi at selected conditions. The crude extracts of Pleurotus sajor-caju (PSC) were selected because of the promising values obtained for the phenoxide formation rate. HRPC/H2O2 and P. sajor-caju derived enzymes/H2O2 (PSC/H2O2) systems produced soluble polyaniline in the presence of polystyrene sulphonated (PES), with high aniline conversions at 45 °C. For the case of insoluble polyphenol production, the PSC-derived enzymes, in absence of hydrogen peroxide, produced insoluble polyphenol with similar efficiencies as those found with HRPC or hematin in a one step phenol treatment (near 40% phenol conversion). For the aniline process, at least 75% aniline conversion was obtained when using PSC enzymes at room temperature. After long reaction times, the lignin-modifying enzymes derived from PSC only produced a conductive form of polyaniline (PANI) at lower temperatures than those required when employing HRPC. Fungal enzymes look promising for eliminating aniline/phenol from wastewaters since the obtained results demonstrated that they are able to polymerizate and precipitate them from aqueous solutions.
publishDate 2006
dc.date.none.fl_str_mv 2006-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37801
Curvetto, Nestor Raul; Figlas, Norma Débora; Brandolin, Adriana; Saidman, Silvana Beatriz; Rueda, Elsa Haydee; et al.; Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water; Elsevier Science Sa; Biochemical Engineering Journal; 29; 3; 4-2006; 191-203
1369-703X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37801
identifier_str_mv Curvetto, Nestor Raul; Figlas, Norma Débora; Brandolin, Adriana; Saidman, Silvana Beatriz; Rueda, Elsa Haydee; et al.; Efficiency of enzymatic and non-enzymatic catalysts in the synthesis of insoluble polyphenol and conductive polyaniline in water; Elsevier Science Sa; Biochemical Engineering Journal; 29; 3; 4-2006; 191-203
1369-703X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bej.2005.11.016
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1369703X05003761
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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