Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge

Autores
Carabajal, Maira Lia; Perullini, Ana Mercedes; Jobbagy, Matias; Ullrich, René; Hofrichter, Martin; Levin, Laura Noemí
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
White-rot fungi have potential in organic pollutant degradation. Immobilization of microorganisms has been successfully used for bioremediation. In this work, 25 isolates of Argentinean white-rot fungi were tested for their tolerance toward up to 10 mM phenol in agar plates. Seven isolates were further evaluated for their ability to grow on plates with 2,6-dimethoxyphenol, gallic acid, 2,4-dichlorophenol, or guaiacol (7.5 mM), or with phenol as sole carbon source. Best results were obtained with Trametes versicolor, Irpex lacteus, Lentinus tigrinus, and Pleurotus lindquistii. The ability of immobilized cultures of T. versicolor BAFC 2234 to remove phenol, was studied. Silica-alginate-fungus biocomposites resulted in phenol removal (10 mM) of up to 48% (mainly attributable to biosorption) within 14 days. Immobilized on Luffa aegyptica, it removed between 62 and 74% of phenol (15 mM) in three repeated cycles over a period of 23 days. Laccase was the main oxidative enzyme detected, and the purified enzyme oxidized 84% of phenol (0.5 mM) in vitro within 4 h, while 43% was converted by a purified Mn-peroxidase. The phenol phytotoxicity decreased noticeably. The concentrations of phenol removed are among the highest reported so far, thus this strain of T. versicolor may have good prospects for application in industrial wastewater treatment.
Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Jobbagy, Matias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ullrich, René. Technische Universität Dresden. Department of Bio- and Environmental Science; Alemania
Fil: Hofrichter, Martin. Technische Universität Dresden. Department of Bio- and Environmental Science; Alemania
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Materia
Laccase
Mn-Peroxidase
Phenolic Compounds
Phytotoxicity
White-Rot Fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26216
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa SpongeCarabajal, Maira LiaPerullini, Ana MercedesJobbagy, MatiasUllrich, RenéHofrichter, MartinLevin, Laura NoemíLaccaseMn-PeroxidasePhenolic CompoundsPhytotoxicityWhite-Rot Fungihttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2White-rot fungi have potential in organic pollutant degradation. Immobilization of microorganisms has been successfully used for bioremediation. In this work, 25 isolates of Argentinean white-rot fungi were tested for their tolerance toward up to 10 mM phenol in agar plates. Seven isolates were further evaluated for their ability to grow on plates with 2,6-dimethoxyphenol, gallic acid, 2,4-dichlorophenol, or guaiacol (7.5 mM), or with phenol as sole carbon source. Best results were obtained with Trametes versicolor, Irpex lacteus, Lentinus tigrinus, and Pleurotus lindquistii. The ability of immobilized cultures of T. versicolor BAFC 2234 to remove phenol, was studied. Silica-alginate-fungus biocomposites resulted in phenol removal (10 mM) of up to 48% (mainly attributable to biosorption) within 14 days. Immobilized on Luffa aegyptica, it removed between 62 and 74% of phenol (15 mM) in three repeated cycles over a period of 23 days. Laccase was the main oxidative enzyme detected, and the purified enzyme oxidized 84% of phenol (0.5 mM) in vitro within 4 h, while 43% was converted by a purified Mn-peroxidase. The phenol phytotoxicity decreased noticeably. The concentrations of phenol removed are among the highest reported so far, thus this strain of T. versicolor may have good prospects for application in industrial wastewater treatment.Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Jobbagy, Matias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ullrich, René. Technische Universität Dresden. Department of Bio- and Environmental Science; AlemaniaFil: Hofrichter, Martin. Technische Universität Dresden. Department of Bio- and Environmental Science; AlemaniaFil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaWiley VCH Verlag2015-12-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26216Carabajal, Maira Lia; Perullini, Ana Mercedes; Jobbagy, Matias; Ullrich, René; Hofrichter, Martin; et al.; Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge; Wiley VCH Verlag; Clean-soil Air Water; 44; 2; 10-12-2015; 180-1881863-0650CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/clen.201400366/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/clen.201400366info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:21Zoai:ri.conicet.gov.ar:11336/26216instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:21.732CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
title Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
spellingShingle Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
Carabajal, Maira Lia
Laccase
Mn-Peroxidase
Phenolic Compounds
Phytotoxicity
White-Rot Fungi
title_short Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
title_full Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
title_fullStr Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
title_full_unstemmed Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
title_sort Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge
dc.creator.none.fl_str_mv Carabajal, Maira Lia
Perullini, Ana Mercedes
Jobbagy, Matias
Ullrich, René
Hofrichter, Martin
Levin, Laura Noemí
author Carabajal, Maira Lia
author_facet Carabajal, Maira Lia
Perullini, Ana Mercedes
Jobbagy, Matias
Ullrich, René
Hofrichter, Martin
Levin, Laura Noemí
author_role author
author2 Perullini, Ana Mercedes
Jobbagy, Matias
Ullrich, René
Hofrichter, Martin
Levin, Laura Noemí
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Laccase
Mn-Peroxidase
Phenolic Compounds
Phytotoxicity
White-Rot Fungi
topic Laccase
Mn-Peroxidase
Phenolic Compounds
Phytotoxicity
White-Rot Fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv White-rot fungi have potential in organic pollutant degradation. Immobilization of microorganisms has been successfully used for bioremediation. In this work, 25 isolates of Argentinean white-rot fungi were tested for their tolerance toward up to 10 mM phenol in agar plates. Seven isolates were further evaluated for their ability to grow on plates with 2,6-dimethoxyphenol, gallic acid, 2,4-dichlorophenol, or guaiacol (7.5 mM), or with phenol as sole carbon source. Best results were obtained with Trametes versicolor, Irpex lacteus, Lentinus tigrinus, and Pleurotus lindquistii. The ability of immobilized cultures of T. versicolor BAFC 2234 to remove phenol, was studied. Silica-alginate-fungus biocomposites resulted in phenol removal (10 mM) of up to 48% (mainly attributable to biosorption) within 14 days. Immobilized on Luffa aegyptica, it removed between 62 and 74% of phenol (15 mM) in three repeated cycles over a period of 23 days. Laccase was the main oxidative enzyme detected, and the purified enzyme oxidized 84% of phenol (0.5 mM) in vitro within 4 h, while 43% was converted by a purified Mn-peroxidase. The phenol phytotoxicity decreased noticeably. The concentrations of phenol removed are among the highest reported so far, thus this strain of T. versicolor may have good prospects for application in industrial wastewater treatment.
Fil: Carabajal, Maira Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Perullini, Ana Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Jobbagy, Matias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ullrich, René. Technische Universität Dresden. Department of Bio- and Environmental Science; Alemania
Fil: Hofrichter, Martin. Technische Universität Dresden. Department of Bio- and Environmental Science; Alemania
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
description White-rot fungi have potential in organic pollutant degradation. Immobilization of microorganisms has been successfully used for bioremediation. In this work, 25 isolates of Argentinean white-rot fungi were tested for their tolerance toward up to 10 mM phenol in agar plates. Seven isolates were further evaluated for their ability to grow on plates with 2,6-dimethoxyphenol, gallic acid, 2,4-dichlorophenol, or guaiacol (7.5 mM), or with phenol as sole carbon source. Best results were obtained with Trametes versicolor, Irpex lacteus, Lentinus tigrinus, and Pleurotus lindquistii. The ability of immobilized cultures of T. versicolor BAFC 2234 to remove phenol, was studied. Silica-alginate-fungus biocomposites resulted in phenol removal (10 mM) of up to 48% (mainly attributable to biosorption) within 14 days. Immobilized on Luffa aegyptica, it removed between 62 and 74% of phenol (15 mM) in three repeated cycles over a period of 23 days. Laccase was the main oxidative enzyme detected, and the purified enzyme oxidized 84% of phenol (0.5 mM) in vitro within 4 h, while 43% was converted by a purified Mn-peroxidase. The phenol phytotoxicity decreased noticeably. The concentrations of phenol removed are among the highest reported so far, thus this strain of T. versicolor may have good prospects for application in industrial wastewater treatment.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26216
Carabajal, Maira Lia; Perullini, Ana Mercedes; Jobbagy, Matias; Ullrich, René; Hofrichter, Martin; et al.; Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge; Wiley VCH Verlag; Clean-soil Air Water; 44; 2; 10-12-2015; 180-188
1863-0650
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26216
identifier_str_mv Carabajal, Maira Lia; Perullini, Ana Mercedes; Jobbagy, Matias; Ullrich, René; Hofrichter, Martin; et al.; Removal of Phenol by Immobilization of Trametes versicolor in Silica–Alginate–Fungus Biocomposites and Loofa Sponge; Wiley VCH Verlag; Clean-soil Air Water; 44; 2; 10-12-2015; 180-188
1863-0650
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/clen.201400366/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/clen.201400366
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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