A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
- Autores
- Mehlenbacher, Shawn A. M; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Gokirmak, Tufan; Bassil, Nahla V.; Kubisiak, Thomas L.
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.
Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados Unidos
Fil: Brown, Rebecca N.. State University of Oregon; Estados Unidos
Fil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gokirmak, Tufan. State University of Oregon; Estados Unidos
Fil: Bassil, Nahla V.. State University of Oregon; Estados Unidos
Fil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados Unidos - Materia
-
Corillus Avellana
Linkage Map
Pseudo Testcross
Simple Sequence Repeat - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/31670
Ver los metadatos del registro completo
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3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markersMehlenbacher, Shawn A. MBrown, Rebecca N.Nouhra, Eduardo RamonGokirmak, TufanBassil, Nahla V.Kubisiak, Thomas L.Corillus AvellanaLinkage MapPseudo TestcrossSimple Sequence Repeathttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados UnidosFil: Brown, Rebecca N.. State University of Oregon; Estados UnidosFil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gokirmak, Tufan. State University of Oregon; Estados UnidosFil: Bassil, Nahla V.. State University of Oregon; Estados UnidosFil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados UnidosNational Research Council Canada-NRC Research Press2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31670Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-1330831-27961480-3321CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://handle.nal.usda.gov/10113/6329info:eu-repo/semantics/altIdentifier/doi/:10.1139/G05-091info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:23Zoai:ri.conicet.gov.ar:11336/31670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:23.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
title |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
spellingShingle |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers Mehlenbacher, Shawn A. M Corillus Avellana Linkage Map Pseudo Testcross Simple Sequence Repeat |
title_short |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
title_full |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
title_fullStr |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
title_full_unstemmed |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
title_sort |
A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers |
dc.creator.none.fl_str_mv |
Mehlenbacher, Shawn A. M Brown, Rebecca N. Nouhra, Eduardo Ramon Gokirmak, Tufan Bassil, Nahla V. Kubisiak, Thomas L. |
author |
Mehlenbacher, Shawn A. M |
author_facet |
Mehlenbacher, Shawn A. M Brown, Rebecca N. Nouhra, Eduardo Ramon Gokirmak, Tufan Bassil, Nahla V. Kubisiak, Thomas L. |
author_role |
author |
author2 |
Brown, Rebecca N. Nouhra, Eduardo Ramon Gokirmak, Tufan Bassil, Nahla V. Kubisiak, Thomas L. |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Corillus Avellana Linkage Map Pseudo Testcross Simple Sequence Repeat |
topic |
Corillus Avellana Linkage Map Pseudo Testcross Simple Sequence Repeat |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations. Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados Unidos Fil: Brown, Rebecca N.. State University of Oregon; Estados Unidos Fil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Gokirmak, Tufan. State University of Oregon; Estados Unidos Fil: Bassil, Nahla V.. State University of Oregon; Estados Unidos Fil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados Unidos |
description |
A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/31670 Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-133 0831-2796 1480-3321 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/31670 |
identifier_str_mv |
Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-133 0831-2796 1480-3321 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://handle.nal.usda.gov/10113/6329 info:eu-repo/semantics/altIdentifier/doi/:10.1139/G05-091 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
National Research Council Canada-NRC Research Press |
publisher.none.fl_str_mv |
National Research Council Canada-NRC Research Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613971968000000 |
score |
13.070432 |