A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers

Autores
Mehlenbacher, Shawn A. M; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Gokirmak, Tufan; Bassil, Nahla V.; Kubisiak, Thomas L.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.
Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados Unidos
Fil: Brown, Rebecca N.. State University of Oregon; Estados Unidos
Fil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gokirmak, Tufan. State University of Oregon; Estados Unidos
Fil: Bassil, Nahla V.. State University of Oregon; Estados Unidos
Fil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados Unidos
Materia
Corillus Avellana
Linkage Map
Pseudo Testcross
Simple Sequence Repeat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31670

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network_name_str CONICET Digital (CONICET)
spelling A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markersMehlenbacher, Shawn A. MBrown, Rebecca N.Nouhra, Eduardo RamonGokirmak, TufanBassil, Nahla V.Kubisiak, Thomas L.Corillus AvellanaLinkage MapPseudo TestcrossSimple Sequence Repeathttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados UnidosFil: Brown, Rebecca N.. State University of Oregon; Estados UnidosFil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Gokirmak, Tufan. State University of Oregon; Estados UnidosFil: Bassil, Nahla V.. State University of Oregon; Estados UnidosFil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados UnidosNational Research Council Canada-NRC Research Press2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31670Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-1330831-27961480-3321CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://handle.nal.usda.gov/10113/6329info:eu-repo/semantics/altIdentifier/doi/:10.1139/G05-091info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:09:23Zoai:ri.conicet.gov.ar:11336/31670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:09:23.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
title A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
spellingShingle A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
Mehlenbacher, Shawn A. M
Corillus Avellana
Linkage Map
Pseudo Testcross
Simple Sequence Repeat
title_short A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
title_full A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
title_fullStr A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
title_full_unstemmed A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
title_sort A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers
dc.creator.none.fl_str_mv Mehlenbacher, Shawn A. M
Brown, Rebecca N.
Nouhra, Eduardo Ramon
Gokirmak, Tufan
Bassil, Nahla V.
Kubisiak, Thomas L.
author Mehlenbacher, Shawn A. M
author_facet Mehlenbacher, Shawn A. M
Brown, Rebecca N.
Nouhra, Eduardo Ramon
Gokirmak, Tufan
Bassil, Nahla V.
Kubisiak, Thomas L.
author_role author
author2 Brown, Rebecca N.
Nouhra, Eduardo Ramon
Gokirmak, Tufan
Bassil, Nahla V.
Kubisiak, Thomas L.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Corillus Avellana
Linkage Map
Pseudo Testcross
Simple Sequence Repeat
topic Corillus Avellana
Linkage Map
Pseudo Testcross
Simple Sequence Repeat
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.
Fil: Mehlenbacher, Shawn A. M. State University of Oregon; Estados Unidos
Fil: Brown, Rebecca N.. State University of Oregon; Estados Unidos
Fil: Nouhra, Eduardo Ramon. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Gokirmak, Tufan. State University of Oregon; Estados Unidos
Fil: Bassil, Nahla V.. State University of Oregon; Estados Unidos
Fil: Kubisiak, Thomas L.. Southern Institute of Forest Genetics; Estados Unidos
description A linkage map for European hazelnut (Corylus avellana L.) was constructed using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers and the 2-way pseudotestcross approach. A full-sib population of 144 seedlings from the cross OSU 252.146 × OSU 414.062 was used. RAPD markers in testcross configuration, segregating 1:1, were used to construct separate maps for each parent. Fifty additional RAPD loci were assigned to linkage groups as accessory markers whose exact location could not be determined. Markers in intercross configuration, segregating 3:1, were used to pair groups in one parent with their homologues in the other. Eleven groups were identified for each parent, corresponding to the haploid chromosome number of hazelnut (n = x = 11). Thirty of the 31 SSR loci were able to be assigned to a linkage group. The maternal map included 249 RAPD and 20 SSR markers and spanned a distance of 661 cM. The paternal map included 271 RAPD and 28 SSR markers and spanned a distance of 812 cM. The maps are quite dense, with an average of 2.6 cM between adjacent markers. The S-locus, which controls pollen?stigma incompatibility, was placed on chromosome 5S where 6 markers linked within a distance of 10 cM were identified. A locus for resistance to eastern filbert blight, caused by Anisogramma anomala, was placed on chromosome 6R for which two additional markers tightly linked to the dominant allele were identified and sequenced. These maps will serve as a starting point for future studies of the hazelnut genome, including map-based cloning of important genes. The inclusion of SSR loci on the map will make it useful in other populations.
publishDate 2006
dc.date.none.fl_str_mv 2006-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31670
Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-133
0831-2796
1480-3321
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31670
identifier_str_mv Kubisiak, Thomas L.; Bassil, Nahla V.; Brown, Rebecca N.; Nouhra, Eduardo Ramon; Mehlenbacher, Shawn A. M; Gokirmak, Tufan; et al.; A genetic linkage map for hazelnut (Corylus avellana L.). based on RAPD and SSR markers; National Research Council Canada-NRC Research Press; Genome; 49; 2; 2-2006; 122-133
0831-2796
1480-3321
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://handle.nal.usda.gov/10113/6329
info:eu-repo/semantics/altIdentifier/doi/:10.1139/G05-091
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
publisher.none.fl_str_mv National Research Council Canada-NRC Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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