Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites

Autores
Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Miranda, Mariana Reneé; Reigada, Chantal; Pereira, Claudio Alejandro
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.
Fil: Camara, Maria de Los Milagros. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Bouvier, Leon Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Reigada, Chantal. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Materia
CHAGAS DISEASE
METACYCLOGENESIS
PARASITE DIFFERENTIATION
INTRACELLULAR EPIMASTIGOTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11566
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasitesCamara, Maria de Los MilagrosBouvier, Leon AlbertoMiranda, Mariana ReneéReigada, ChantalPereira, Claudio AlejandroCHAGAS DISEASEMETACYCLOGENESISPARASITE DIFFERENTIATIONINTRACELLULAR EPIMASTIGOTEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.Fil: Camara, Maria de Los Milagros. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Bouvier, Leon Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Reigada, Chantal. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; ArgentinaFolia Parasitologica2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11566Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Miranda, Mariana Reneé; Reigada, Chantal; Pereira, Claudio Alejandro; Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites; Folia Parasitologica; Folia Parasitologica; 60; 5; 3-2013; 406-4100015-5683enginfo:eu-repo/semantics/altIdentifier/url/http://folia.paru.cas.cz/artkey/fol-201305-0003_Laboratory_techniques_to_obtain_different_forms_of_Trypanosoma_cruzi_applications_to_wild-type_and_genetically.phpinfo:eu-repo/semantics/altIdentifier/doi/10.14411/fp.2013.042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-06-10T10:02:10Zoai:ri.conicet.gov.ar:11336/11566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-06-10 10:02:10.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
title Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
spellingShingle Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
Camara, Maria de Los Milagros
CHAGAS DISEASE
METACYCLOGENESIS
PARASITE DIFFERENTIATION
INTRACELLULAR EPIMASTIGOTES
title_short Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
title_full Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
title_fullStr Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
title_full_unstemmed Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
title_sort Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites
dc.creator.none.fl_str_mv Camara, Maria de Los Milagros
Bouvier, Leon Alberto
Miranda, Mariana Reneé
Reigada, Chantal
Pereira, Claudio Alejandro
author Camara, Maria de Los Milagros
author_facet Camara, Maria de Los Milagros
Bouvier, Leon Alberto
Miranda, Mariana Reneé
Reigada, Chantal
Pereira, Claudio Alejandro
author_role author
author2 Bouvier, Leon Alberto
Miranda, Mariana Reneé
Reigada, Chantal
Pereira, Claudio Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv CHAGAS DISEASE
METACYCLOGENESIS
PARASITE DIFFERENTIATION
INTRACELLULAR EPIMASTIGOTES
topic CHAGAS DISEASE
METACYCLOGENESIS
PARASITE DIFFERENTIATION
INTRACELLULAR EPIMASTIGOTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.
Fil: Camara, Maria de Los Milagros. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Bouvier, Leon Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Miranda, Mariana Reneé. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Reigada, Chantal. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
Fil: Pereira, Claudio Alejandro. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Investigaciones Medicas; Argentina. Universidad de Buenos Aires; Argentina
description Nowadays, there are no simple techniques for mimicking in vitro the life cycle of the kinetoplasmtid Trypanosoma cruzi Chagas, 1909, causative agent of Chagas disease, especially for parasite strains maintained as epimastigotes for many years. In the present study, we propose a method for obtaining metacyclic trypomastigotes, which were capable of infecting mammalian cells by simply lowering pH media. The collected amastigotes and trypomastigotes were differentiated into epimastigotes closing T. cruzi life cycle in vitro. Metacyclogenesis rates and infectivity were enhanced in cycled parasites. Finally, using this method, we were able to infect cells with transgenic parasites obtaining trypomastigotes and amastigotes using a neomycin-resistant cell line.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11566
Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Miranda, Mariana Reneé; Reigada, Chantal; Pereira, Claudio Alejandro; Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites; Folia Parasitologica; Folia Parasitologica; 60; 5; 3-2013; 406-410
0015-5683
url http://hdl.handle.net/11336/11566
identifier_str_mv Camara, Maria de Los Milagros; Bouvier, Leon Alberto; Miranda, Mariana Reneé; Reigada, Chantal; Pereira, Claudio Alejandro; Laboratory techniques to obtain different forms of Trypanosoma cruzi: applications to wild-type and genetically modified parasites; Folia Parasitologica; Folia Parasitologica; 60; 5; 3-2013; 406-410
0015-5683
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://folia.paru.cas.cz/artkey/fol-201305-0003_Laboratory_techniques_to_obtain_different_forms_of_Trypanosoma_cruzi_applications_to_wild-type_and_genetically.php
info:eu-repo/semantics/altIdentifier/doi/10.14411/fp.2013.042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Folia Parasitologica
publisher.none.fl_str_mv Folia Parasitologica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.98848

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