Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi

Autores
Svagzdys, Ailin; Vilchez Larrea, Salomé Catalina; Fernandez Villamil, Silvia Hebe
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poly(ADP-ribosyl)ation is a post-translational modification catalyzed by poly(ADP- ribose)polymerases (PARPs). As a reversible modification, it is regulated by a delicate balance ofsynthesis and degradation, and is involved in various nuclear processes related to DNAmetabolism. To adapt to its complex life cycle, T. cruzi undergoes several differentiationprocesses such as metacyclogenesis, where the replicative epimastigote transforms into theinfective trypomastigote. Here we evaluated the role of TcPARP during T. cruzi metacyclogenesisusing Olaparib, a specific PARP inhibitor, with two differentiation techniques: TAU medium andblood agar. Our results using two different T. cruzi strains show that, depending on thedifferentiation technique used, the inhibition of TcPARP stimulates or hinders metacyclogenesis.We also compared the formation of reactive oxygen species (ROS) and autophagosomes duringthe induction of metacyclogenesis on blood agar, in order to understand the pathways that arebeing triggered in this process. Preliminary results indicate that while ROS formation could betriggering the metacyclogenesis in blood agar, autophagy would not be involved in this process.In order to further study this process, we are generating TcPARP knockout cell lines that will helpus corroborate our results.
Fil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Parasitravaganza
Smithfield
Australia
The Australian Society for Parasitology
Materia
TRYPANSOMA CRUZI
PARP
METACYCLOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/153825
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Role of TcPARP during the metacyclogenesis of Trypanosoma cruziSvagzdys, AilinVilchez Larrea, Salomé CatalinaFernandez Villamil, Silvia HebeTRYPANSOMA CRUZIPARPMETACYCLOGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Poly(ADP-ribosyl)ation is a post-translational modification catalyzed by poly(ADP- ribose)polymerases (PARPs). As a reversible modification, it is regulated by a delicate balance ofsynthesis and degradation, and is involved in various nuclear processes related to DNAmetabolism. To adapt to its complex life cycle, T. cruzi undergoes several differentiationprocesses such as metacyclogenesis, where the replicative epimastigote transforms into theinfective trypomastigote. Here we evaluated the role of TcPARP during T. cruzi metacyclogenesisusing Olaparib, a specific PARP inhibitor, with two differentiation techniques: TAU medium andblood agar. Our results using two different T. cruzi strains show that, depending on thedifferentiation technique used, the inhibition of TcPARP stimulates or hinders metacyclogenesis.We also compared the formation of reactive oxygen species (ROS) and autophagosomes duringthe induction of metacyclogenesis on blood agar, in order to understand the pathways that arebeing triggered in this process. Preliminary results indicate that while ROS formation could betriggering the metacyclogenesis in blood agar, autophagy would not be involved in this process.In order to further study this process, we are generating TcPARP knockout cell lines that will helpus corroborate our results.Fil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaParasitravaganzaSmithfieldAustraliaThe Australian Society for ParasitologyThe Australian Society for Parasitology2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/153825Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi; Parasitravaganza; Smithfield; Australia; 2020; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-3.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:40Zoai:ri.conicet.gov.ar:11336/153825instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:41.2CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
title Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
spellingShingle Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
Svagzdys, Ailin
TRYPANSOMA CRUZI
PARP
METACYCLOGENESIS
title_short Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
title_full Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
title_fullStr Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
title_full_unstemmed Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
title_sort Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi
dc.creator.none.fl_str_mv Svagzdys, Ailin
Vilchez Larrea, Salomé Catalina
Fernandez Villamil, Silvia Hebe
author Svagzdys, Ailin
author_facet Svagzdys, Ailin
Vilchez Larrea, Salomé Catalina
Fernandez Villamil, Silvia Hebe
author_role author
author2 Vilchez Larrea, Salomé Catalina
Fernandez Villamil, Silvia Hebe
author2_role author
author
dc.subject.none.fl_str_mv TRYPANSOMA CRUZI
PARP
METACYCLOGENESIS
topic TRYPANSOMA CRUZI
PARP
METACYCLOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Poly(ADP-ribosyl)ation is a post-translational modification catalyzed by poly(ADP- ribose)polymerases (PARPs). As a reversible modification, it is regulated by a delicate balance ofsynthesis and degradation, and is involved in various nuclear processes related to DNAmetabolism. To adapt to its complex life cycle, T. cruzi undergoes several differentiationprocesses such as metacyclogenesis, where the replicative epimastigote transforms into theinfective trypomastigote. Here we evaluated the role of TcPARP during T. cruzi metacyclogenesisusing Olaparib, a specific PARP inhibitor, with two differentiation techniques: TAU medium andblood agar. Our results using two different T. cruzi strains show that, depending on thedifferentiation technique used, the inhibition of TcPARP stimulates or hinders metacyclogenesis.We also compared the formation of reactive oxygen species (ROS) and autophagosomes duringthe induction of metacyclogenesis on blood agar, in order to understand the pathways that arebeing triggered in this process. Preliminary results indicate that while ROS formation could betriggering the metacyclogenesis in blood agar, autophagy would not be involved in this process.In order to further study this process, we are generating TcPARP knockout cell lines that will helpus corroborate our results.
Fil: Svagzdys, Ailin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vilchez Larrea, Salomé Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Fernandez Villamil, Silvia Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Parasitravaganza
Smithfield
Australia
The Australian Society for Parasitology
description Poly(ADP-ribosyl)ation is a post-translational modification catalyzed by poly(ADP- ribose)polymerases (PARPs). As a reversible modification, it is regulated by a delicate balance ofsynthesis and degradation, and is involved in various nuclear processes related to DNAmetabolism. To adapt to its complex life cycle, T. cruzi undergoes several differentiationprocesses such as metacyclogenesis, where the replicative epimastigote transforms into theinfective trypomastigote. Here we evaluated the role of TcPARP during T. cruzi metacyclogenesisusing Olaparib, a specific PARP inhibitor, with two differentiation techniques: TAU medium andblood agar. Our results using two different T. cruzi strains show that, depending on thedifferentiation technique used, the inhibition of TcPARP stimulates or hinders metacyclogenesis.We also compared the formation of reactive oxygen species (ROS) and autophagosomes duringthe induction of metacyclogenesis on blood agar, in order to understand the pathways that arebeing triggered in this process. Preliminary results indicate that while ROS formation could betriggering the metacyclogenesis in blood agar, autophagy would not be involved in this process.In order to further study this process, we are generating TcPARP knockout cell lines that will helpus corroborate our results.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/153825
Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi; Parasitravaganza; Smithfield; Australia; 2020; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/153825
identifier_str_mv Role of TcPARP during the metacyclogenesis of Trypanosoma cruzi; Parasitravaganza; Smithfield; Australia; 2020; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.parasite.org.au/wp-content/uploads/2020/07/Parasitravaganza-Program-Booklet-3.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv The Australian Society for Parasitology
publisher.none.fl_str_mv The Australian Society for Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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