Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber

Autores
Lanteri, Maria Luciana; Laxalt, Ana Maria; Lamattina, Lorenzo
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Auxin and nitric oxide (NO) play fundamental roles throughout plant life. NO is a second messenger in auxin signal transductionleading to root developmental processes. The mechanisms triggered by auxin and NO that direct adventitious root (AR)formation are beginning to be unraveled. The goal of this work was to study phospholipid (PL) signaling during the auxin- andNO-induced AR formation in cucumber (Cucumis sativus) explants. Explants were labeled with 32P-inorganic phosphate andtreated with the auxins indole-3-acetic acid or 1-naphthylacetic acid, or the NO donor S-nitroso N-acetyl penicillamine, in thepresence or absence of the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. PLs wereseparated by thin-layer chromatography and quantified. We report that the signaling PLs phosphatidic acid (PA), phosphatidylinositolphosphate, and phosphatidylinositol bisphosphate accumulated within 1 min after auxin or NO treatment. Bothauxin and NO evoked similar and transient time course responses, since signaling PLs returned to control levels after 20 or30 min of treatment. The results indicate that auxin relies on NO in inducing PA, phosphatidylinositol phosphate, andphosphatidylinositol bisphosphate accumulation. Furthermore, we demonstrate that auxin and NO trigger PA formation viaphospholipase D (PLD) activity. Explants treated for 10 min with auxin or NO displayed a 200% increase in AR numbercompared with control explants. In addition, PLD activity was required for the auxin- and NO-induced AR formation. Finally,exogenously applied PA increased up to 300% the number of ARs. Altogether, our data support the idea that PLD-derived PA isan early signaling event during AR formation induced by auxin and NO in cucumber explants.
Fil: Lanteri, Maria Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
adventitious root
phosphatidic acid
nitric oxide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133981
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/133981
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumberLanteri, Maria LucianaLaxalt, Ana MariaLamattina, Lorenzoadventitious rootphosphatidic acidnitric oxidehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Auxin and nitric oxide (NO) play fundamental roles throughout plant life. NO is a second messenger in auxin signal transductionleading to root developmental processes. The mechanisms triggered by auxin and NO that direct adventitious root (AR)formation are beginning to be unraveled. The goal of this work was to study phospholipid (PL) signaling during the auxin- andNO-induced AR formation in cucumber (Cucumis sativus) explants. Explants were labeled with 32P-inorganic phosphate andtreated with the auxins indole-3-acetic acid or 1-naphthylacetic acid, or the NO donor S-nitroso N-acetyl penicillamine, in thepresence or absence of the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. PLs wereseparated by thin-layer chromatography and quantified. We report that the signaling PLs phosphatidic acid (PA), phosphatidylinositolphosphate, and phosphatidylinositol bisphosphate accumulated within 1 min after auxin or NO treatment. Bothauxin and NO evoked similar and transient time course responses, since signaling PLs returned to control levels after 20 or30 min of treatment. The results indicate that auxin relies on NO in inducing PA, phosphatidylinositol phosphate, andphosphatidylinositol bisphosphate accumulation. Furthermore, we demonstrate that auxin and NO trigger PA formation viaphospholipase D (PLD) activity. Explants treated for 10 min with auxin or NO displayed a 200% increase in AR numbercompared with control explants. In addition, PLD activity was required for the auxin- and NO-induced AR formation. Finally,exogenously applied PA increased up to 300% the number of ARs. Altogether, our data support the idea that PLD-derived PA isan early signaling event during AR formation induced by auxin and NO in cucumber explants.Fil: Lanteri, Maria Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaAmerican Society of Plant Biologist2008-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133981Lanteri, Maria Luciana; Laxalt, Ana Maria; Lamattina, Lorenzo; Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber; American Society of Plant Biologist; Plant Physiology; 147; 1; 7-2008; 188-1980032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1104/pp.107.111815info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/147/1/188/6107430info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:37Zoai:ri.conicet.gov.ar:11336/133981instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:37.797CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
title Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
spellingShingle Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
Lanteri, Maria Luciana
adventitious root
phosphatidic acid
nitric oxide
title_short Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
title_full Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
title_fullStr Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
title_full_unstemmed Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
title_sort Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber
dc.creator.none.fl_str_mv Lanteri, Maria Luciana
Laxalt, Ana Maria
Lamattina, Lorenzo
author Lanteri, Maria Luciana
author_facet Lanteri, Maria Luciana
Laxalt, Ana Maria
Lamattina, Lorenzo
author_role author
author2 Laxalt, Ana Maria
Lamattina, Lorenzo
author2_role author
author
dc.subject.none.fl_str_mv adventitious root
phosphatidic acid
nitric oxide
topic adventitious root
phosphatidic acid
nitric oxide
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Auxin and nitric oxide (NO) play fundamental roles throughout plant life. NO is a second messenger in auxin signal transductionleading to root developmental processes. The mechanisms triggered by auxin and NO that direct adventitious root (AR)formation are beginning to be unraveled. The goal of this work was to study phospholipid (PL) signaling during the auxin- andNO-induced AR formation in cucumber (Cucumis sativus) explants. Explants were labeled with 32P-inorganic phosphate andtreated with the auxins indole-3-acetic acid or 1-naphthylacetic acid, or the NO donor S-nitroso N-acetyl penicillamine, in thepresence or absence of the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. PLs wereseparated by thin-layer chromatography and quantified. We report that the signaling PLs phosphatidic acid (PA), phosphatidylinositolphosphate, and phosphatidylinositol bisphosphate accumulated within 1 min after auxin or NO treatment. Bothauxin and NO evoked similar and transient time course responses, since signaling PLs returned to control levels after 20 or30 min of treatment. The results indicate that auxin relies on NO in inducing PA, phosphatidylinositol phosphate, andphosphatidylinositol bisphosphate accumulation. Furthermore, we demonstrate that auxin and NO trigger PA formation viaphospholipase D (PLD) activity. Explants treated for 10 min with auxin or NO displayed a 200% increase in AR numbercompared with control explants. In addition, PLD activity was required for the auxin- and NO-induced AR formation. Finally,exogenously applied PA increased up to 300% the number of ARs. Altogether, our data support the idea that PLD-derived PA isan early signaling event during AR formation induced by auxin and NO in cucumber explants.
Fil: Lanteri, Maria Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Laxalt, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Auxin and nitric oxide (NO) play fundamental roles throughout plant life. NO is a second messenger in auxin signal transductionleading to root developmental processes. The mechanisms triggered by auxin and NO that direct adventitious root (AR)formation are beginning to be unraveled. The goal of this work was to study phospholipid (PL) signaling during the auxin- andNO-induced AR formation in cucumber (Cucumis sativus) explants. Explants were labeled with 32P-inorganic phosphate andtreated with the auxins indole-3-acetic acid or 1-naphthylacetic acid, or the NO donor S-nitroso N-acetyl penicillamine, in thepresence or absence of the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide. PLs wereseparated by thin-layer chromatography and quantified. We report that the signaling PLs phosphatidic acid (PA), phosphatidylinositolphosphate, and phosphatidylinositol bisphosphate accumulated within 1 min after auxin or NO treatment. Bothauxin and NO evoked similar and transient time course responses, since signaling PLs returned to control levels after 20 or30 min of treatment. The results indicate that auxin relies on NO in inducing PA, phosphatidylinositol phosphate, andphosphatidylinositol bisphosphate accumulation. Furthermore, we demonstrate that auxin and NO trigger PA formation viaphospholipase D (PLD) activity. Explants treated for 10 min with auxin or NO displayed a 200% increase in AR numbercompared with control explants. In addition, PLD activity was required for the auxin- and NO-induced AR formation. Finally,exogenously applied PA increased up to 300% the number of ARs. Altogether, our data support the idea that PLD-derived PA isan early signaling event during AR formation induced by auxin and NO in cucumber explants.
publishDate 2008
dc.date.none.fl_str_mv 2008-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133981
Lanteri, Maria Luciana; Laxalt, Ana Maria; Lamattina, Lorenzo; Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber; American Society of Plant Biologist; Plant Physiology; 147; 1; 7-2008; 188-198
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133981
identifier_str_mv Lanteri, Maria Luciana; Laxalt, Ana Maria; Lamattina, Lorenzo; Nitric oxide triggers phosphatidic acid accumulation via phospholipase D during auxin-induced adventitious root formation in cucumber; American Society of Plant Biologist; Plant Physiology; 147; 1; 7-2008; 188-198
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.107.111815
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/147/1/188/6107430
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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