A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide

Autores
Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; Penichet, Manuel L.; Chen, Irvin S.Y.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.
Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Daniels, Tracy R.. University of California at Los Angeles; Estados Unidos
Fil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados Unidos
Materia
BIOTIN ADAPTOR PEPTIDE
ENDOTHELIAL CELLS
LENTIVIRAL VECTOR
SINDBIS VIRUS ENVELOPE
TARGETING VECTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/149130

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network_name_str CONICET Digital (CONICET)
spelling A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptideMorizono, KoukiXie, YimingHelguera, Gustavo FernandoDaniels, Tracy R.Lane T. F.Penichet, Manuel L.Chen, Irvin S.Y.BIOTIN ADAPTOR PEPTIDEENDOTHELIAL CELLSLENTIVIRAL VECTORSINDBIS VIRUS ENVELOPETARGETING VECTORhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Daniels, Tracy R.. University of California at Los Angeles; Estados UnidosFil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados UnidosJohn Wiley & Sons Ltd2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149130Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-6631099-498XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/jgm.1345info:eu-repo/semantics/altIdentifier/doi/10.1002/jgm.1345info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:13Zoai:ri.conicet.gov.ar:11336/149130instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:13.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
title A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
spellingShingle A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
Morizono, Kouki
BIOTIN ADAPTOR PEPTIDE
ENDOTHELIAL CELLS
LENTIVIRAL VECTOR
SINDBIS VIRUS ENVELOPE
TARGETING VECTOR
title_short A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
title_full A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
title_fullStr A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
title_full_unstemmed A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
title_sort A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
dc.creator.none.fl_str_mv Morizono, Kouki
Xie, Yiming
Helguera, Gustavo Fernando
Daniels, Tracy R.
Lane T. F.
Penichet, Manuel L.
Chen, Irvin S.Y.
author Morizono, Kouki
author_facet Morizono, Kouki
Xie, Yiming
Helguera, Gustavo Fernando
Daniels, Tracy R.
Lane T. F.
Penichet, Manuel L.
Chen, Irvin S.Y.
author_role author
author2 Xie, Yiming
Helguera, Gustavo Fernando
Daniels, Tracy R.
Lane T. F.
Penichet, Manuel L.
Chen, Irvin S.Y.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOTIN ADAPTOR PEPTIDE
ENDOTHELIAL CELLS
LENTIVIRAL VECTOR
SINDBIS VIRUS ENVELOPE
TARGETING VECTOR
topic BIOTIN ADAPTOR PEPTIDE
ENDOTHELIAL CELLS
LENTIVIRAL VECTOR
SINDBIS VIRUS ENVELOPE
TARGETING VECTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.
Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Daniels, Tracy R.. University of California at Los Angeles; Estados Unidos
Fil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados Unidos
description Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.
publishDate 2009
dc.date.none.fl_str_mv 2009-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/149130
Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-663
1099-498X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/149130
identifier_str_mv Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-663
1099-498X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/jgm.1345
info:eu-repo/semantics/altIdentifier/doi/10.1002/jgm.1345
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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