A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide
- Autores
- Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; Penichet, Manuel L.; Chen, Irvin S.Y.
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.
Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Daniels, Tracy R.. University of California at Los Angeles; Estados Unidos
Fil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados Unidos
Fil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados Unidos - Materia
-
BIOTIN ADAPTOR PEPTIDE
ENDOTHELIAL CELLS
LENTIVIRAL VECTOR
SINDBIS VIRUS ENVELOPE
TARGETING VECTOR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/149130
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/149130 |
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A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptideMorizono, KoukiXie, YimingHelguera, Gustavo FernandoDaniels, Tracy R.Lane T. F.Penichet, Manuel L.Chen, Irvin S.Y.BIOTIN ADAPTOR PEPTIDEENDOTHELIAL CELLSLENTIVIRAL VECTORSINDBIS VIRUS ENVELOPETARGETING VECTORhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin.Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Daniels, Tracy R.. University of California at Los Angeles; Estados UnidosFil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados UnidosFil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados UnidosJohn Wiley & Sons Ltd2009-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/149130Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-6631099-498XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/jgm.1345info:eu-repo/semantics/altIdentifier/doi/10.1002/jgm.1345info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:13Zoai:ri.conicet.gov.ar:11336/149130instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:13.782CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
title |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
spellingShingle |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide Morizono, Kouki BIOTIN ADAPTOR PEPTIDE ENDOTHELIAL CELLS LENTIVIRAL VECTOR SINDBIS VIRUS ENVELOPE TARGETING VECTOR |
title_short |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
title_full |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
title_fullStr |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
title_full_unstemmed |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
title_sort |
A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide |
dc.creator.none.fl_str_mv |
Morizono, Kouki Xie, Yiming Helguera, Gustavo Fernando Daniels, Tracy R. Lane T. F. Penichet, Manuel L. Chen, Irvin S.Y. |
author |
Morizono, Kouki |
author_facet |
Morizono, Kouki Xie, Yiming Helguera, Gustavo Fernando Daniels, Tracy R. Lane T. F. Penichet, Manuel L. Chen, Irvin S.Y. |
author_role |
author |
author2 |
Xie, Yiming Helguera, Gustavo Fernando Daniels, Tracy R. Lane T. F. Penichet, Manuel L. Chen, Irvin S.Y. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BIOTIN ADAPTOR PEPTIDE ENDOTHELIAL CELLS LENTIVIRAL VECTOR SINDBIS VIRUS ENVELOPE TARGETING VECTOR |
topic |
BIOTIN ADAPTOR PEPTIDE ENDOTHELIAL CELLS LENTIVIRAL VECTOR SINDBIS VIRUS ENVELOPE TARGETING VECTOR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin. Fil: Morizono, Kouki. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: Xie, Yiming. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: Helguera, Gustavo Fernando. University of California at Los Angeles; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Daniels, Tracy R.. University of California at Los Angeles; Estados Unidos Fil: Lane T. F.. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: Penichet, Manuel L.. University of California at Los Angeles; Estados Unidos. University of California at Los Angeles. School of Medicine; Estados Unidos Fil: Chen, Irvin S.Y.. University of California at Los Angeles. School of Medicine; Estados Unidos |
description |
Background: Targeted gene transduction in vivo is the ultimate preferred method for gene delivery. We previously developed targeting lentiviral vectors that specifically recognize cell surface molecules with conjugated antibodies and mediate targeted gene transduction both in vitro and in vivo. Although effective in some experimental settings, the conjugation of virus with antibodies is mediated by the interaction between protein A and the Fc region of antibodies, which is not as stable as covalent conjugation. We have now developed a more stable conjugation strategy utilizing the interaction between avidin and biotin. Methods: We inserted the biotin-adaptor-peptide, which was biotinylated by secretory biotin ligase at specific sites, into our targeting envelope proteins, enabling conjugation of the pseudotyped virus with avidin, streptavidin or neutravidin. Results: When conjugated with avidin-antibody fusion proteins or the complex of avidin and biotinylated targeting molecules, the vectors could mediate specific transduction to targeted cells recognized by the targeting molecules. When conjugated with streptavidin-coated magnetic beads, transduction by the vectors was targeted to the locations of magnets. Conclusions: This targeting vector system can be used for broad applications of targeted gene transduction using biotinylated targeting molecules or targeting molecules fused with avidin. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/149130 Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-663 1099-498X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/149130 |
identifier_str_mv |
Morizono, Kouki; Xie, Yiming; Helguera, Gustavo Fernando; Daniels, Tracy R.; Lane T. F.; et al.; A versatile targeting system with lentiviral vectors bearing the biotin-adaptor peptide; John Wiley & Sons Ltd; Journal Of Gene Medicine; 11; 8; 5-2009; 655-663 1099-498X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/jgm.1345 info:eu-repo/semantics/altIdentifier/doi/10.1002/jgm.1345 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons Ltd |
publisher.none.fl_str_mv |
John Wiley & Sons Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614067210158080 |
score |
13.070432 |