Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues

Autores
Radovic, Slobodanka; Rapisarda, Viviana Andrea; Tosato, Valentina; Bruschi, Carlo V.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Expression of yeast RuvB-like gene analogues of bacterial RuvB is self-regulated, as episomal overexpression of RVB1 and RVB2 decreases the expression of their chromosomal copies by 85%. Heterozygosity for either gene correlates with lower double-strand break repair of inverted-repeat DNA and decreased survival after UV irradiation, suggesting their haploinsufficiency, while overexpression of the bacterial RuvAB complex improves UV survival in yeast. Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA. As both proteins also interact with chromatin, their role in recombination and repair through chromatin remodelling, and their evolutionary relationship to the bacterial homologue, is discussed.
Fil: Radovic, Slobodanka. Yeast Molecular Genetics Group; Italia
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Yeast Molecular Genetics Group; Italia
Fil: Tosato, Valentina. Yeast Molecular Genetics Group; Italia
Fil: Bruschi, Carlo V.. Yeast Molecular Genetics Group; Italia
Materia
DNA HOLLIDAY JUNCTION
HOMOLOGOUS RECOMBINATION
RUVB-LIKE GENES
UV DAMAGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95108
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95108
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologuesRadovic, SlobodankaRapisarda, Viviana AndreaTosato, ValentinaBruschi, Carlo V.DNA HOLLIDAY JUNCTIONHOMOLOGOUS RECOMBINATIONRUVB-LIKE GENESUV DAMAGEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Expression of yeast RuvB-like gene analogues of bacterial RuvB is self-regulated, as episomal overexpression of RVB1 and RVB2 decreases the expression of their chromosomal copies by 85%. Heterozygosity for either gene correlates with lower double-strand break repair of inverted-repeat DNA and decreased survival after UV irradiation, suggesting their haploinsufficiency, while overexpression of the bacterial RuvAB complex improves UV survival in yeast. Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA. As both proteins also interact with chromatin, their role in recombination and repair through chromatin remodelling, and their evolutionary relationship to the bacterial homologue, is discussed.Fil: Radovic, Slobodanka. Yeast Molecular Genetics Group; ItaliaFil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Yeast Molecular Genetics Group; ItaliaFil: Tosato, Valentina. Yeast Molecular Genetics Group; ItaliaFil: Bruschi, Carlo V.. Yeast Molecular Genetics Group; ItaliaWiley Blackwell Publishing, Inc2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95108Radovic, Slobodanka; Rapisarda, Viviana Andrea; Tosato, Valentina; Bruschi, Carlo V.; Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues; Wiley Blackwell Publishing, Inc; Fems Yeast Research; 7; 4; 6-2007; 527-5391567-1356CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/article/7/4/527/623169info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1567-1364.2006.00205.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1567-1364.2006.00205.x?sid=nlm%3Apubmedinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:21Zoai:ri.conicet.gov.ar:11336/95108instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:21.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
title Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
spellingShingle Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
Radovic, Slobodanka
DNA HOLLIDAY JUNCTION
HOMOLOGOUS RECOMBINATION
RUVB-LIKE GENES
UV DAMAGE
title_short Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
title_full Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
title_fullStr Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
title_full_unstemmed Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
title_sort Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues
dc.creator.none.fl_str_mv Radovic, Slobodanka
Rapisarda, Viviana Andrea
Tosato, Valentina
Bruschi, Carlo V.
author Radovic, Slobodanka
author_facet Radovic, Slobodanka
Rapisarda, Viviana Andrea
Tosato, Valentina
Bruschi, Carlo V.
author_role author
author2 Rapisarda, Viviana Andrea
Tosato, Valentina
Bruschi, Carlo V.
author2_role author
author
author
dc.subject.none.fl_str_mv DNA HOLLIDAY JUNCTION
HOMOLOGOUS RECOMBINATION
RUVB-LIKE GENES
UV DAMAGE
topic DNA HOLLIDAY JUNCTION
HOMOLOGOUS RECOMBINATION
RUVB-LIKE GENES
UV DAMAGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Expression of yeast RuvB-like gene analogues of bacterial RuvB is self-regulated, as episomal overexpression of RVB1 and RVB2 decreases the expression of their chromosomal copies by 85%. Heterozygosity for either gene correlates with lower double-strand break repair of inverted-repeat DNA and decreased survival after UV irradiation, suggesting their haploinsufficiency, while overexpression of the bacterial RuvAB complex improves UV survival in yeast. Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA. As both proteins also interact with chromatin, their role in recombination and repair through chromatin remodelling, and their evolutionary relationship to the bacterial homologue, is discussed.
Fil: Radovic, Slobodanka. Yeast Molecular Genetics Group; Italia
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Yeast Molecular Genetics Group; Italia
Fil: Tosato, Valentina. Yeast Molecular Genetics Group; Italia
Fil: Bruschi, Carlo V.. Yeast Molecular Genetics Group; Italia
description Expression of yeast RuvB-like gene analogues of bacterial RuvB is self-regulated, as episomal overexpression of RVB1 and RVB2 decreases the expression of their chromosomal copies by 85%. Heterozygosity for either gene correlates with lower double-strand break repair of inverted-repeat DNA and decreased survival after UV irradiation, suggesting their haploinsufficiency, while overexpression of the bacterial RuvAB complex improves UV survival in yeast. Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA. As both proteins also interact with chromatin, their role in recombination and repair through chromatin remodelling, and their evolutionary relationship to the bacterial homologue, is discussed.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95108
Radovic, Slobodanka; Rapisarda, Viviana Andrea; Tosato, Valentina; Bruschi, Carlo V.; Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues; Wiley Blackwell Publishing, Inc; Fems Yeast Research; 7; 4; 6-2007; 527-539
1567-1356
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95108
identifier_str_mv Radovic, Slobodanka; Rapisarda, Viviana Andrea; Tosato, Valentina; Bruschi, Carlo V.; Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues; Wiley Blackwell Publishing, Inc; Fems Yeast Research; 7; 4; 6-2007; 527-539
1567-1356
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsyr/article/7/4/527/623169
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1567-1364.2006.00205.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1567-1364.2006.00205.x?sid=nlm%3Apubmed
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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