Differential contribution of HP1 proteins to DNA end resection and homology-directed repair
- Autores
- Soria, Ramiro Gaston; Almouzni, Geneviéve
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Heterochromatin protein 1 paralogs (HP1α, β and γ in mammals) are not only central in heterochromatin organization, but have also been linked to transcriptional activation at euchromatic regions, maintenance of telomere stability and, most recently, to the DNA damage response (DDR). However, how HP1 proteins contribute to the DDR at a molecular level, and whether HP1 paralogs within the same organism, as well as their respective orthologs, have overlapping or unique roles in the DDR, remain to be elucidated. Herein, we have combined the analysis of the efficiency and kinetics of recruitment of key repair proteins to sites of DNA damage with specific DNA repair assays to demonstrate that human HP1 paralogs differentially modulate homology-directed repair (HDR) pathways, including homologous recombination (HR) and single-strand annealing (SSA). We find that while HP1α and β stimulate HR and SSA, HP1γ has an inhibitory role. In addition, we show that the stimulatory role of HP1α and β in HDR is linked to the DNA-end resection step of DNA breaks, through the promotion of RPA loading and phosphorylation at damage sites. Altogether, our findings provide mechanistic insight into how human HP1 proteins participate in the recombination process, emerging as important chromatin regulators during HDR.
Fil: Soria, Ramiro Gaston. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Almouzni, Geneviéve. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; Francia - Materia
-
Heterochromatin
Dna Repair
Dna Damage Response
Homologous Recombination - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/7557
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Differential contribution of HP1 proteins to DNA end resection and homology-directed repairSoria, Ramiro GastonAlmouzni, GeneviéveHeterochromatinDna RepairDna Damage ResponseHomologous Recombinationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heterochromatin protein 1 paralogs (HP1α, β and γ in mammals) are not only central in heterochromatin organization, but have also been linked to transcriptional activation at euchromatic regions, maintenance of telomere stability and, most recently, to the DNA damage response (DDR). However, how HP1 proteins contribute to the DDR at a molecular level, and whether HP1 paralogs within the same organism, as well as their respective orthologs, have overlapping or unique roles in the DDR, remain to be elucidated. Herein, we have combined the analysis of the efficiency and kinetics of recruitment of key repair proteins to sites of DNA damage with specific DNA repair assays to demonstrate that human HP1 paralogs differentially modulate homology-directed repair (HDR) pathways, including homologous recombination (HR) and single-strand annealing (SSA). We find that while HP1α and β stimulate HR and SSA, HP1γ has an inhibitory role. In addition, we show that the stimulatory role of HP1α and β in HDR is linked to the DNA-end resection step of DNA breaks, through the promotion of RPA loading and phosphorylation at damage sites. Altogether, our findings provide mechanistic insight into how human HP1 proteins participate in the recombination process, emerging as important chromatin regulators during HDR.Fil: Soria, Ramiro Gaston. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Almouzni, Geneviéve. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; FranciaTaylor & Francis2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7557Soria, Ramiro Gaston; Almouzni, Geneviéve; Differential contribution of HP1 proteins to DNA end resection and homology-directed repair; Taylor & Francis; Cell Cycle; 12; 2-2013; 422-4291538-4101enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/cc.23215info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587443/info:eu-repo/semantics/altIdentifier/doi/10.4161/cc.23215info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:15:38Zoai:ri.conicet.gov.ar:11336/7557instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:15:38.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
title |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
spellingShingle |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair Soria, Ramiro Gaston Heterochromatin Dna Repair Dna Damage Response Homologous Recombination |
title_short |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
title_full |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
title_fullStr |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
title_full_unstemmed |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
title_sort |
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair |
dc.creator.none.fl_str_mv |
Soria, Ramiro Gaston Almouzni, Geneviéve |
author |
Soria, Ramiro Gaston |
author_facet |
Soria, Ramiro Gaston Almouzni, Geneviéve |
author_role |
author |
author2 |
Almouzni, Geneviéve |
author2_role |
author |
dc.subject.none.fl_str_mv |
Heterochromatin Dna Repair Dna Damage Response Homologous Recombination |
topic |
Heterochromatin Dna Repair Dna Damage Response Homologous Recombination |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Heterochromatin protein 1 paralogs (HP1α, β and γ in mammals) are not only central in heterochromatin organization, but have also been linked to transcriptional activation at euchromatic regions, maintenance of telomere stability and, most recently, to the DNA damage response (DDR). However, how HP1 proteins contribute to the DDR at a molecular level, and whether HP1 paralogs within the same organism, as well as their respective orthologs, have overlapping or unique roles in the DDR, remain to be elucidated. Herein, we have combined the analysis of the efficiency and kinetics of recruitment of key repair proteins to sites of DNA damage with specific DNA repair assays to demonstrate that human HP1 paralogs differentially modulate homology-directed repair (HDR) pathways, including homologous recombination (HR) and single-strand annealing (SSA). We find that while HP1α and β stimulate HR and SSA, HP1γ has an inhibitory role. In addition, we show that the stimulatory role of HP1α and β in HDR is linked to the DNA-end resection step of DNA breaks, through the promotion of RPA loading and phosphorylation at damage sites. Altogether, our findings provide mechanistic insight into how human HP1 proteins participate in the recombination process, emerging as important chromatin regulators during HDR. Fil: Soria, Ramiro Gaston. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Almouzni, Geneviéve. Centre National de la Recherche Scientifique; Francia. Institut Curie Section Recherche; Francia |
description |
Heterochromatin protein 1 paralogs (HP1α, β and γ in mammals) are not only central in heterochromatin organization, but have also been linked to transcriptional activation at euchromatic regions, maintenance of telomere stability and, most recently, to the DNA damage response (DDR). However, how HP1 proteins contribute to the DDR at a molecular level, and whether HP1 paralogs within the same organism, as well as their respective orthologs, have overlapping or unique roles in the DDR, remain to be elucidated. Herein, we have combined the analysis of the efficiency and kinetics of recruitment of key repair proteins to sites of DNA damage with specific DNA repair assays to demonstrate that human HP1 paralogs differentially modulate homology-directed repair (HDR) pathways, including homologous recombination (HR) and single-strand annealing (SSA). We find that while HP1α and β stimulate HR and SSA, HP1γ has an inhibitory role. In addition, we show that the stimulatory role of HP1α and β in HDR is linked to the DNA-end resection step of DNA breaks, through the promotion of RPA loading and phosphorylation at damage sites. Altogether, our findings provide mechanistic insight into how human HP1 proteins participate in the recombination process, emerging as important chromatin regulators during HDR. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/7557 Soria, Ramiro Gaston; Almouzni, Geneviéve; Differential contribution of HP1 proteins to DNA end resection and homology-directed repair; Taylor & Francis; Cell Cycle; 12; 2-2013; 422-429 1538-4101 |
url |
http://hdl.handle.net/11336/7557 |
identifier_str_mv |
Soria, Ramiro Gaston; Almouzni, Geneviéve; Differential contribution of HP1 proteins to DNA end resection and homology-directed repair; Taylor & Francis; Cell Cycle; 12; 2-2013; 422-429 1538-4101 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.4161/cc.23215 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587443/ info:eu-repo/semantics/altIdentifier/doi/10.4161/cc.23215 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |