Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat

Autores
Kippes, Néstor Fabián; Zhu, Jie; Chen, Andrew; Vanzetti, Leonardo Sebastián; Lukaszewski, Adam; Nishida, Hidetaka; Kato, Kenji; Dvorak, Jan; Dubcovsky, Jorge
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene.
Fil: Kippes, Néstor Fabián. University of California at Davis; Estados Unidos
Fil: Zhu, Jie. Washington State University; Estados Unidos
Fil: Chen, Andrew. University of California at Davis; Estados Unidos
Fil: Vanzetti, Leonardo Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juarez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lukaszewski, Adam. University of California; Estados Unidos
Fil: Nishida, Hidetaka. Okayama University; Japón
Fil: Kato, Kenji. Okayama University; Japón
Fil: Dvorak, Jan. University of California at Davis; Estados Unidos
Fil: Dubcovsky, Jorge. University of California at Davis; Estados Unidos
Materia
Wheat
Vernalization
Vrn-D4
Vrn1
Genetic Mapping
Vil
Flowering
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28515
Acceder
id CONICETDig_c5606f1d90e2b24d7e71baf0261b8d91
oai_identifier_str oai:ri.conicet.gov.ar:11336/28515
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheatKippes, Néstor FabiánZhu, JieChen, AndrewVanzetti, Leonardo SebastiánLukaszewski, AdamNishida, HidetakaKato, KenjiDvorak, JanDubcovsky, JorgeWheatVernalizationVrn-D4Vrn1Genetic MappingVilFloweringhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene.Fil: Kippes, Néstor Fabián. University of California at Davis; Estados UnidosFil: Zhu, Jie. Washington State University; Estados UnidosFil: Chen, Andrew. University of California at Davis; Estados UnidosFil: Vanzetti, Leonardo Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juarez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lukaszewski, Adam. University of California; Estados UnidosFil: Nishida, Hidetaka. Okayama University; JapónFil: Kato, Kenji. Okayama University; JapónFil: Dvorak, Jan. University of California at Davis; Estados UnidosFil: Dubcovsky, Jorge. University of California at Davis; Estados UnidosSpringer2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28515Kippes, Néstor Fabián; Zhu, Jie; Chen, Andrew; Vanzetti, Leonardo Sebastián; Lukaszewski, Adam; et al.; Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat; Springer; Molecular Genetics And Genomics; 289; 1; 11-2013; 47-621617-46151617-4623CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00438-013-0788-yinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00438-013-0788-yinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916708/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:20Zoai:ri.conicet.gov.ar:11336/28515instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:20.349CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
title Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
spellingShingle Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
Kippes, Néstor Fabián
Wheat
Vernalization
Vrn-D4
Vrn1
Genetic Mapping
Vil
Flowering
title_short Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
title_full Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
title_fullStr Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
title_full_unstemmed Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
title_sort Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat
dc.creator.none.fl_str_mv Kippes, Néstor Fabián
Zhu, Jie
Chen, Andrew
Vanzetti, Leonardo Sebastián
Lukaszewski, Adam
Nishida, Hidetaka
Kato, Kenji
Dvorak, Jan
Dubcovsky, Jorge
author Kippes, Néstor Fabián
author_facet Kippes, Néstor Fabián
Zhu, Jie
Chen, Andrew
Vanzetti, Leonardo Sebastián
Lukaszewski, Adam
Nishida, Hidetaka
Kato, Kenji
Dvorak, Jan
Dubcovsky, Jorge
author_role author
author2 Zhu, Jie
Chen, Andrew
Vanzetti, Leonardo Sebastián
Lukaszewski, Adam
Nishida, Hidetaka
Kato, Kenji
Dvorak, Jan
Dubcovsky, Jorge
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Wheat
Vernalization
Vrn-D4
Vrn1
Genetic Mapping
Vil
Flowering
topic Wheat
Vernalization
Vrn-D4
Vrn1
Genetic Mapping
Vil
Flowering
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene.
Fil: Kippes, Néstor Fabián. University of California at Davis; Estados Unidos
Fil: Zhu, Jie. Washington State University; Estados Unidos
Fil: Chen, Andrew. University of California at Davis; Estados Unidos
Fil: Vanzetti, Leonardo Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Córdoba. Estación Experimental Agropecuaria Marcos Juarez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lukaszewski, Adam. University of California; Estados Unidos
Fil: Nishida, Hidetaka. Okayama University; Japón
Fil: Kato, Kenji. Okayama University; Japón
Fil: Dvorak, Jan. University of California at Davis; Estados Unidos
Fil: Dubcovsky, Jorge. University of California at Davis; Estados Unidos
description Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28515
Kippes, Néstor Fabián; Zhu, Jie; Chen, Andrew; Vanzetti, Leonardo Sebastián; Lukaszewski, Adam; et al.; Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat; Springer; Molecular Genetics And Genomics; 289; 1; 11-2013; 47-62
1617-4615
1617-4623
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28515
identifier_str_mv Kippes, Néstor Fabián; Zhu, Jie; Chen, Andrew; Vanzetti, Leonardo Sebastián; Lukaszewski, Adam; et al.; Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat; Springer; Molecular Genetics And Genomics; 289; 1; 11-2013; 47-62
1617-4615
1617-4623
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00438-013-0788-y
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00438-013-0788-y
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916708/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981170837454848
score 12.48226

Publicaciones similares