Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions

Autores
Madueño, Laura; Alvarez, Hector Manuel; Morelli, Irma S.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.
Fil: Madueño, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morelli, Irma S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Materia
Polycyclic Aromatic Hydrocarbons
Autochthonus Bioaugmentation
Sphingobium
Patagonian Soil
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16070
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditionsMadueño, LauraAlvarez, Hector ManuelMorelli, Irma S.Polycyclic Aromatic HydrocarbonsAutochthonus BioaugmentationSphingobiumPatagonian Soilhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.Fil: Madueño, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morelli, Irma S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaSpringer2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16070Madueño, Laura; Alvarez, Hector Manuel; Morelli, Irma S.; Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions; Springer; International Journal Of Environmental Science And Technology; 12; 7; 7-2014; 2317-23261735-14721735-2630enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s13762-014-0637-5info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13762-014-0637-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:37:40Zoai:ri.conicet.gov.ar:11336/16070instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:37:41.222CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
title Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
spellingShingle Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
Madueño, Laura
Polycyclic Aromatic Hydrocarbons
Autochthonus Bioaugmentation
Sphingobium
Patagonian Soil
title_short Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
title_full Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
title_fullStr Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
title_full_unstemmed Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
title_sort Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions
dc.creator.none.fl_str_mv Madueño, Laura
Alvarez, Hector Manuel
Morelli, Irma S.
author Madueño, Laura
author_facet Madueño, Laura
Alvarez, Hector Manuel
Morelli, Irma S.
author_role author
author2 Alvarez, Hector Manuel
Morelli, Irma S.
author2_role author
author
dc.subject.none.fl_str_mv Polycyclic Aromatic Hydrocarbons
Autochthonus Bioaugmentation
Sphingobium
Patagonian Soil
topic Polycyclic Aromatic Hydrocarbons
Autochthonus Bioaugmentation
Sphingobium
Patagonian Soil
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.
Fil: Madueño, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina
Fil: Alvarez, Hector Manuel. Universidad Nacional de la Patagonia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morelli, Irma S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
description The aim of this work was to investigate the effect of autochthonous bioaugmentation (ABA) in phenanthrene-contaminated Patagonian soil microcosms, maintained under arid conditions, on phenanthrene elimination and soil microbial community. The polycyclic aromatic hydrocarbon (PAH)-degrading strain Sphingobium sp. 22B previously isolated from the Patagonian soil and selected by its resistance to drying conditions was used as inoculant. The phenanthrene concentration, dehydrogenase activity and denaturing gradient electrophoresis of 16S rRNA gene were monitored during 230 days. The results showed that when the microcosms were maintained at 20 % of soil water-holding capacity (WHC), the phenanthrene biodegradation was drastically inhibited and changes in the genetic diversity of soil microbial community were not detected, and neither the ABA nor the biostimulation managed to overcome the inhibitory effects. When the moisture was slightly increased, reached 25 % WHC, the ABA showed a significant initial stimulatory effect on phenanthrene biodegradation, demonstrating the potential of ABA in PAH bioremediation process in semiarid Patagonia.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16070
Madueño, Laura; Alvarez, Hector Manuel; Morelli, Irma S.; Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions; Springer; International Journal Of Environmental Science And Technology; 12; 7; 7-2014; 2317-2326
1735-1472
1735-2630
url http://hdl.handle.net/11336/16070
identifier_str_mv Madueño, Laura; Alvarez, Hector Manuel; Morelli, Irma S.; Autochthonous bioaugmentation to enhance phenanthrene degradation in soil microcosms under arid conditions; Springer; International Journal Of Environmental Science And Technology; 12; 7; 7-2014; 2317-2326
1735-1472
1735-2630
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s13762-014-0637-5
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs13762-014-0637-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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