Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments

Autores
Pedetta, Andrea; Pouyte, Karina; Herrera Seitz, Karina; Babay, Paola Alejandra; Espinosa, Mariela; Costagliola, Marcela; Studdert, Claudia Alicia; Peressutti, Silvia R.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150mgl-1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75-100% in 72-168h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas.
Fil: Pedetta, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pouyte, Karina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Espinosa, Mariela. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Costagliola, Marcela. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Peressutti, Silvia R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Materia
BIOREMEDIATION
BIOSURFACTANT
CHEMOTAXIS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100952
Acceder
id CONICETDig_e449f596a5e020c461bc4c7573c326cd
oai_identifier_str oai:ri.conicet.gov.ar:11336/100952
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sedimentsPedetta, AndreaPouyte, KarinaHerrera Seitz, KarinaBabay, Paola AlejandraEspinosa, MarielaCostagliola, MarcelaStuddert, Claudia AliciaPeressutti, Silvia R.BIOREMEDIATIONBIOSURFACTANTCHEMOTAXISPHENANTHRENEPOLYCYCLIC AROMATIC HYDROCARBONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150mgl-1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75-100% in 72-168h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas.Fil: Pedetta, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pouyte, Karina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Espinosa, Mariela. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; ArgentinaFil: Costagliola, Marcela. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaFil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Peressutti, Silvia R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; ArgentinaElsevier2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100952Pedetta, Andrea; Pouyte, Karina; Herrera Seitz, Karina; Babay, Paola Alejandra; Espinosa, Mariela; et al.; Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments; Elsevier; International Biodeterioration and Biodegradation; 84; 10-2013; 161-1670964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512001795info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.04.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:56Zoai:ri.conicet.gov.ar:11336/100952instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:56.973CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
title Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
spellingShingle Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
Pedetta, Andrea
BIOREMEDIATION
BIOSURFACTANT
CHEMOTAXIS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBON
title_short Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
title_full Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
title_fullStr Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
title_full_unstemmed Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
title_sort Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments
dc.creator.none.fl_str_mv Pedetta, Andrea
Pouyte, Karina
Herrera Seitz, Karina
Babay, Paola Alejandra
Espinosa, Mariela
Costagliola, Marcela
Studdert, Claudia Alicia
Peressutti, Silvia R.
author Pedetta, Andrea
author_facet Pedetta, Andrea
Pouyte, Karina
Herrera Seitz, Karina
Babay, Paola Alejandra
Espinosa, Mariela
Costagliola, Marcela
Studdert, Claudia Alicia
Peressutti, Silvia R.
author_role author
author2 Pouyte, Karina
Herrera Seitz, Karina
Babay, Paola Alejandra
Espinosa, Mariela
Costagliola, Marcela
Studdert, Claudia Alicia
Peressutti, Silvia R.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOREMEDIATION
BIOSURFACTANT
CHEMOTAXIS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBON
topic BIOREMEDIATION
BIOSURFACTANT
CHEMOTAXIS
PHENANTHRENE
POLYCYCLIC AROMATIC HYDROCARBON
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150mgl-1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75-100% in 72-168h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas.
Fil: Pedetta, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pouyte, Karina. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Herrera Seitz, Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Babay, Paola Alejandra. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Espinosa, Mariela. Comisión Nacional de Energía Atómica. Centro Atómico Constituyentes; Argentina
Fil: Costagliola, Marcela. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
Fil: Studdert, Claudia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Peressutti, Silvia R.. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina
description Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150mgl-1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75-100% in 72-168h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100952
Pedetta, Andrea; Pouyte, Karina; Herrera Seitz, Karina; Babay, Paola Alejandra; Espinosa, Mariela; et al.; Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments; Elsevier; International Biodeterioration and Biodegradation; 84; 10-2013; 161-167
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100952
identifier_str_mv Pedetta, Andrea; Pouyte, Karina; Herrera Seitz, Karina; Babay, Paola Alejandra; Espinosa, Mariela; et al.; Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments; Elsevier; International Biodeterioration and Biodegradation; 84; 10-2013; 161-167
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830512001795
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.04.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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