Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing

Autores
Festa, Sabrina; Macchi, Marianela; Cortés, Federico; Morelli, Irma Susana; Coppotelli, Bibiana Marina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on Actinomycetales and Sphingomonadales orders, while inoculation caused a relative decline of Actinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of Sphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86746
Acceder
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network_name_str SEDICI (UNLP)
spelling Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencingFesta, SabrinaMacchi, MarianelaCortés, FedericoMorelli, Irma SusanaCoppotelli, Bibiana MarinaCiencias ExactasBioaugmentationCommunity diversityInoculant establishmentPyrosequencingSoil microbiomeSphingobium sp. AM genomeThe effect of bioaugmentation with <i>Sphingobium</i> sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on <i>Actinomycetales</i> and <i>Sphingomonadales</i> orders, while inoculation caused a relative decline of <i>Actinomycetales</i>. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of <i>Sphingomonadales</i>, <i>Burkholderiales</i> and <i>Rhizobiales</i> orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones Industriales2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86746enginfo:eu-repo/semantics/altIdentifier/issn/0168-6496info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiw125info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:08Zoai:sedici.unlp.edu.ar:10915/86746Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:08.798SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
spellingShingle Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
Festa, Sabrina
Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
title_short Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_full Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_fullStr Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_full_unstemmed Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
title_sort Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing
dc.creator.none.fl_str_mv Festa, Sabrina
Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author Festa, Sabrina
author_facet Festa, Sabrina
Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author_role author
author2 Macchi, Marianela
Cortés, Federico
Morelli, Irma Susana
Coppotelli, Bibiana Marina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
topic Ciencias Exactas
Bioaugmentation
Community diversity
Inoculant establishment
Pyrosequencing
Soil microbiome
Sphingobium sp. AM genome
dc.description.none.fl_txt_mv The effect of bioaugmentation with <i>Sphingobium</i> sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on <i>Actinomycetales</i> and <i>Sphingomonadales</i> orders, while inoculation caused a relative decline of <i>Actinomycetales</i>. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of <i>Sphingomonadales</i>, <i>Burkholderiales</i> and <i>Rhizobiales</i> orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description The effect of bioaugmentation with <i>Sphingobium</i> sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on <i>Actinomycetales</i> and <i>Sphingomonadales</i> orders, while inoculation caused a relative decline of <i>Actinomycetales</i>. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of <i>Sphingomonadales</i>, <i>Burkholderiales</i> and <i>Rhizobiales</i> orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86746
url http://sedici.unlp.edu.ar/handle/10915/86746
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0168-6496
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiw125
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.13397

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