Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction

Autores
Pisaneschi, Silvia; Strigini, Francesca A. L.; Sanchez, Angel Matias; Begliuomini, Silvia; Casarosa, Elena; Ripoli, Andrea; Ghirri, Paolo; Boldrini, Antonio; Fink, Bruno; Genazzani, Andrea R.; Coceani, Flavio; Simoncini, Tommaso
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods: Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results: Umbilical blood nitrite (p<0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while asymmetric dimethylarginine decreased (p = 0.003). Nitrite rise coincided with an abnormal Doppler profile from umbilical arteries. Fetal growth restriction umbilical vein endothelial cells produced more nitrite and also exhibited reciprocal changes in vasodilator (upwards) and vasoconstrictor (downwards) transcripts. Elevation in blood nitrite and S-nitrosohemoglobin persisted postnatally in the fetal growth restriction offspring. Conclusion: Fetal growth restriction is typified by increased nitric oxide production during pregnancy and after birth. This response is viewed as an adaptative event to sustain placental blood flow. However, its occurrence may modify the endothelial phenotype and may ultimately represent an element of risk for cardiovascular disease in adult life.
Fil: Pisaneschi, Silvia. Università degli Studi di Pisa; Italia. Scuola Superiore Sant’Anna; Italia
Fil: Strigini, Francesca A. L.. Università degli Studi di Pisa; Italia
Fil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Begliuomini, Silvia. Università degli Studi di Pisa; Italia
Fil: Casarosa, Elena. Università degli Studi di Pisa; Italia
Fil: Ripoli, Andrea. National Research Council. Institute of Clinical Physiology, ; Italia
Fil: Ghirri, Paolo. Università degli Studi di Pisa; Italia
Fil: Boldrini, Antonio. Università degli Studi di Pisa; Italia
Fil: Fink, Bruno. Noxygen Science Transfer and Diagnostics; Alemania
Fil: Genazzani, Andrea R.. Università degli Studi di Pisa; Italia
Fil: Coceani, Flavio. Scuola Superiore Sant’Anna; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
Materia
Fetal Growth Restriction
nitric oxide system
S- nitrosohemoglobin
Endothelial cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80368
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/80368
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth RestrictionPisaneschi, SilviaStrigini, Francesca A. L.Sanchez, Angel MatiasBegliuomini, SilviaCasarosa, ElenaRipoli, AndreaGhirri, PaoloBoldrini, AntonioFink, BrunoGenazzani, Andrea R.Coceani, FlavioSimoncini, TommasoFetal Growth Restrictionnitric oxide systemS- nitrosohemoglobinEndothelial cellshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background: Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods: Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results: Umbilical blood nitrite (p<0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while asymmetric dimethylarginine decreased (p = 0.003). Nitrite rise coincided with an abnormal Doppler profile from umbilical arteries. Fetal growth restriction umbilical vein endothelial cells produced more nitrite and also exhibited reciprocal changes in vasodilator (upwards) and vasoconstrictor (downwards) transcripts. Elevation in blood nitrite and S-nitrosohemoglobin persisted postnatally in the fetal growth restriction offspring. Conclusion: Fetal growth restriction is typified by increased nitric oxide production during pregnancy and after birth. This response is viewed as an adaptative event to sustain placental blood flow. However, its occurrence may modify the endothelial phenotype and may ultimately represent an element of risk for cardiovascular disease in adult life.Fil: Pisaneschi, Silvia. Università degli Studi di Pisa; Italia. Scuola Superiore Sant’Anna; ItaliaFil: Strigini, Francesca A. L.. Università degli Studi di Pisa; ItaliaFil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Begliuomini, Silvia. Università degli Studi di Pisa; ItaliaFil: Casarosa, Elena. Università degli Studi di Pisa; ItaliaFil: Ripoli, Andrea. National Research Council. Institute of Clinical Physiology, ; ItaliaFil: Ghirri, Paolo. Università degli Studi di Pisa; ItaliaFil: Boldrini, Antonio. Università degli Studi di Pisa; ItaliaFil: Fink, Bruno. Noxygen Science Transfer and Diagnostics; AlemaniaFil: Genazzani, Andrea R.. Università degli Studi di Pisa; ItaliaFil: Coceani, Flavio. Scuola Superiore Sant’Anna; ItaliaFil: Simoncini, Tommaso. Università degli Studi di Pisa; ItaliaPublic Library of Science2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80368Pisaneschi, Silvia; Strigini, Francesca A. L.; Sanchez, Angel Matias; Begliuomini, Silvia; Casarosa, Elena; et al.; Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction; Public Library of Science; Plos One; 7; 9; 9-2012; 1-9; e452941932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0045294info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0045294info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:32Zoai:ri.conicet.gov.ar:11336/80368instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:32.765CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
title Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
spellingShingle Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
Pisaneschi, Silvia
Fetal Growth Restriction
nitric oxide system
S- nitrosohemoglobin
Endothelial cells
title_short Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
title_full Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
title_fullStr Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
title_full_unstemmed Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
title_sort Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction
dc.creator.none.fl_str_mv Pisaneschi, Silvia
Strigini, Francesca A. L.
Sanchez, Angel Matias
Begliuomini, Silvia
Casarosa, Elena
Ripoli, Andrea
Ghirri, Paolo
Boldrini, Antonio
Fink, Bruno
Genazzani, Andrea R.
Coceani, Flavio
Simoncini, Tommaso
author Pisaneschi, Silvia
author_facet Pisaneschi, Silvia
Strigini, Francesca A. L.
Sanchez, Angel Matias
Begliuomini, Silvia
Casarosa, Elena
Ripoli, Andrea
Ghirri, Paolo
Boldrini, Antonio
Fink, Bruno
Genazzani, Andrea R.
Coceani, Flavio
Simoncini, Tommaso
author_role author
author2 Strigini, Francesca A. L.
Sanchez, Angel Matias
Begliuomini, Silvia
Casarosa, Elena
Ripoli, Andrea
Ghirri, Paolo
Boldrini, Antonio
Fink, Bruno
Genazzani, Andrea R.
Coceani, Flavio
Simoncini, Tommaso
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fetal Growth Restriction
nitric oxide system
S- nitrosohemoglobin
Endothelial cells
topic Fetal Growth Restriction
nitric oxide system
S- nitrosohemoglobin
Endothelial cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods: Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results: Umbilical blood nitrite (p<0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while asymmetric dimethylarginine decreased (p = 0.003). Nitrite rise coincided with an abnormal Doppler profile from umbilical arteries. Fetal growth restriction umbilical vein endothelial cells produced more nitrite and also exhibited reciprocal changes in vasodilator (upwards) and vasoconstrictor (downwards) transcripts. Elevation in blood nitrite and S-nitrosohemoglobin persisted postnatally in the fetal growth restriction offspring. Conclusion: Fetal growth restriction is typified by increased nitric oxide production during pregnancy and after birth. This response is viewed as an adaptative event to sustain placental blood flow. However, its occurrence may modify the endothelial phenotype and may ultimately represent an element of risk for cardiovascular disease in adult life.
Fil: Pisaneschi, Silvia. Università degli Studi di Pisa; Italia. Scuola Superiore Sant’Anna; Italia
Fil: Strigini, Francesca A. L.. Università degli Studi di Pisa; Italia
Fil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Begliuomini, Silvia. Università degli Studi di Pisa; Italia
Fil: Casarosa, Elena. Università degli Studi di Pisa; Italia
Fil: Ripoli, Andrea. National Research Council. Institute of Clinical Physiology, ; Italia
Fil: Ghirri, Paolo. Università degli Studi di Pisa; Italia
Fil: Boldrini, Antonio. Università degli Studi di Pisa; Italia
Fil: Fink, Bruno. Noxygen Science Transfer and Diagnostics; Alemania
Fil: Genazzani, Andrea R.. Università degli Studi di Pisa; Italia
Fil: Coceani, Flavio. Scuola Superiore Sant’Anna; Italia
Fil: Simoncini, Tommaso. Università degli Studi di Pisa; Italia
description Background: Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods: Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results: Umbilical blood nitrite (p<0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while asymmetric dimethylarginine decreased (p = 0.003). Nitrite rise coincided with an abnormal Doppler profile from umbilical arteries. Fetal growth restriction umbilical vein endothelial cells produced more nitrite and also exhibited reciprocal changes in vasodilator (upwards) and vasoconstrictor (downwards) transcripts. Elevation in blood nitrite and S-nitrosohemoglobin persisted postnatally in the fetal growth restriction offspring. Conclusion: Fetal growth restriction is typified by increased nitric oxide production during pregnancy and after birth. This response is viewed as an adaptative event to sustain placental blood flow. However, its occurrence may modify the endothelial phenotype and may ultimately represent an element of risk for cardiovascular disease in adult life.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80368
Pisaneschi, Silvia; Strigini, Francesca A. L.; Sanchez, Angel Matias; Begliuomini, Silvia; Casarosa, Elena; et al.; Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction; Public Library of Science; Plos One; 7; 9; 9-2012; 1-9; e45294
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80368
identifier_str_mv Pisaneschi, Silvia; Strigini, Francesca A. L.; Sanchez, Angel Matias; Begliuomini, Silvia; Casarosa, Elena; et al.; Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction; Public Library of Science; Plos One; 7; 9; 9-2012; 1-9; e45294
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0045294
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0045294
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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