Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System

Autores
Lencina, Franco Daniel Alberto; Prina, Alberto R.; Pacheco, Maria Gabriela; Kobayashi, Ken; Landau, Alejandra Mabel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance.
Fil: Lencina, Franco Daniel Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Prina, Alberto R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kobayashi, Ken. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Materia
PLASTOME
CHLOROPLAST DNA
ORGANELLES
ILLEGITIMATE RECOMBINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276964
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276964
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR SystemLencina, Franco Daniel AlbertoPrina, Alberto R.Pacheco, Maria GabrielaKobayashi, KenLandau, Alejandra MabelPLASTOMECHLOROPLAST DNAORGANELLESILLEGITIMATE RECOMBINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance.Fil: Lencina, Franco Daniel Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Prina, Alberto R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kobayashi, Ken. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaMolecular Diversity Preservation International2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276964Lencina, Franco Daniel Alberto; Prina, Alberto R.; Pacheco, Maria Gabriela; Kobayashi, Ken; Landau, Alejandra Mabel; Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 17; 9-2025; 8644-86601422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/17/8644info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26178644info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:36:24Zoai:ri.conicet.gov.ar:11336/276964instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:36:24.8CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
title Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
spellingShingle Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
Lencina, Franco Daniel Alberto
PLASTOME
CHLOROPLAST DNA
ORGANELLES
ILLEGITIMATE RECOMBINATION
title_short Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
title_full Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
title_fullStr Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
title_full_unstemmed Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
title_sort Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System
dc.creator.none.fl_str_mv Lencina, Franco Daniel Alberto
Prina, Alberto R.
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
author Lencina, Franco Daniel Alberto
author_facet Lencina, Franco Daniel Alberto
Prina, Alberto R.
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
author_role author
author2 Prina, Alberto R.
Pacheco, Maria Gabriela
Kobayashi, Ken
Landau, Alejandra Mabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv PLASTOME
CHLOROPLAST DNA
ORGANELLES
ILLEGITIMATE RECOMBINATION
topic PLASTOME
CHLOROPLAST DNA
ORGANELLES
ILLEGITIMATE RECOMBINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance.
Fil: Lencina, Franco Daniel Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Prina, Alberto R.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kobayashi, Ken. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
description A mutation detection strategy based on mismatch digestion was applied previously in barley seedlings carrying the chloroplast mutator (cpm) genotype through many generations. Sixty-one mutations were detected along with four large indels: a 15 bp insertion in the intergenic region between tRNAHis and rps19 genes, a 620 bp deletion in the psbA gene, a 79 bp deletion in the intergenic region between rpl33 and rps18 genes and a 45 bp deletion in the rps3 gene. The present investigation aims to understand the mechanisms producing the large indels and to better characterize the cpm mutagenic effect. Whole plastome sequencing revealed novel polymorphisms that were identified either in regions not previously examined or in regions that were explored but not detected through celery juice extract (CJE) digestion. The 620 bp deletion in the psbA gene was lethal when homoplastomic, whereas the 45 bp deletion in the rps3 gene did not affect the viability of the seedlings even in homoplastomy. The presence of direct repeats at the borders of large indels suggests that they could have originated by illegitimate recombination because of CPM protein malfunction. A truncated mismatch repair MSH1 protein identified in cpm seedlings suggests that CPM is involved in organellar genome stability maintenance.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276964
Lencina, Franco Daniel Alberto; Prina, Alberto R.; Pacheco, Maria Gabriela; Kobayashi, Ken; Landau, Alejandra Mabel; Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 17; 9-2025; 8644-8660
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276964
identifier_str_mv Lencina, Franco Daniel Alberto; Prina, Alberto R.; Pacheco, Maria Gabriela; Kobayashi, Ken; Landau, Alejandra Mabel; Four Large Indels in Barley Chloroplast Mutator (cpm) Seedlings Reinforce the Hypothesis of a Malfunction in the MMR System; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 26; 17; 9-2025; 8644-8660
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/26/17/8644
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms26178644
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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