The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings

Autores
Lencina, Franco; Landau, Alejandra Mabel; Petterson, Maria Elizabeth; Pacheco, Maria Gabriela; Kobayashi, K.; Prina, Alberto Raul
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that t
Instituto de Genética
Fil: Lencina, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Petterson, Maria Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fuente
Scientific report 9 (1) : 9960. (10 July 2019)
Materia
Pseudogenes
Chloroplasts
Mutagenesis
Recombination
Barley
Vitroplants
Polymorphism
Seudogenes
Cloroplasto
Mutagénesis
Genes
Recombinación
Cebada
Vitroplantas
Polimorfismo
Tilling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6293
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6293
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network_name_str INTA Digital (INTA)
spelling The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlingsLencina, FrancoLandau, Alejandra MabelPetterson, Maria ElizabethPacheco, Maria GabrielaKobayashi, K.Prina, Alberto RaulPseudogenesChloroplastsMutagenesisRecombinationBarleyVitroplantsPolymorphismSeudogenesCloroplastoMutagénesisGenesRecombinaciónCebadaVitroplantasPolimorfismoTillingPreviously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that tInstituto de GenéticaFil: Lencina, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Petterson, Maria Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaNature Publishing Group2019-11-05T18:47:14Z2019-11-05T18:47:14Z2019-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.nature.com/articles/s41598-019-46321-6http://hdl.handle.net/20.500.12123/62932045-2322https://doi.org/10.1038/s41598-019-46321-6Scientific report 9 (1) : 9960. (10 July 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:49Zoai:localhost:20.500.12123/6293instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:49.361INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
title The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
spellingShingle The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
Lencina, Franco
Pseudogenes
Chloroplasts
Mutagenesis
Recombination
Barley
Vitroplants
Polymorphism
Seudogenes
Cloroplasto
Mutagénesis
Genes
Recombinación
Cebada
Vitroplantas
Polimorfismo
Tilling
title_short The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
title_full The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
title_fullStr The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
title_full_unstemmed The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
title_sort The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings
dc.creator.none.fl_str_mv Lencina, Franco
Landau, Alejandra Mabel
Petterson, Maria Elizabeth
Pacheco, Maria Gabriela
Kobayashi, K.
Prina, Alberto Raul
author Lencina, Franco
author_facet Lencina, Franco
Landau, Alejandra Mabel
Petterson, Maria Elizabeth
Pacheco, Maria Gabriela
Kobayashi, K.
Prina, Alberto Raul
author_role author
author2 Landau, Alejandra Mabel
Petterson, Maria Elizabeth
Pacheco, Maria Gabriela
Kobayashi, K.
Prina, Alberto Raul
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pseudogenes
Chloroplasts
Mutagenesis
Recombination
Barley
Vitroplants
Polymorphism
Seudogenes
Cloroplasto
Mutagénesis
Genes
Recombinación
Cebada
Vitroplantas
Polimorfismo
Tilling
topic Pseudogenes
Chloroplasts
Mutagenesis
Recombination
Barley
Vitroplants
Polymorphism
Seudogenes
Cloroplasto
Mutagénesis
Genes
Recombinación
Cebada
Vitroplantas
Polimorfismo
Tilling
dc.description.none.fl_txt_mv Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that t
Instituto de Genética
Fil: Lencina, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Petterson, Maria Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Pacheco, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada. Departamento de Fisiología, Biología Molecular y Celular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
description Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that the detected polymorphisms initially arose from gene conversion between gene and pseudogene. Moreover, an additional recombination process involving small recombinant segments seems to occur between the two gene copies as a consequence of their location in the IRs. These and previous results support the hypothesis that t
publishDate 2019
dc.date.none.fl_str_mv 2019-11-05T18:47:14Z
2019-11-05T18:47:14Z
2019-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.nature.com/articles/s41598-019-46321-6
http://hdl.handle.net/20.500.12123/6293
2045-2322
https://doi.org/10.1038/s41598-019-46321-6
url https://www.nature.com/articles/s41598-019-46321-6
http://hdl.handle.net/20.500.12123/6293
https://doi.org/10.1038/s41598-019-46321-6
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO/1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Scientific report 9 (1) : 9960. (10 July 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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