The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene

Autores
Lencina, Franco Daniel; Landau, Alejandra Mabel; Prina, Alberto Raul
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences.
Instituto de Genética
Fil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
International Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022)
Materia
Barley
Chloroplasts
Mutants
Cebada
Cloroplasto
Mutantes
Barley Chloroplast Mutator
Msh1 gene
Plastome
Mutante del Cloroplasto de Cebada
Gen Msh1
Plastoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11564
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11564
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 geneLencina, Franco DanielLandau, Alejandra MabelPrina, Alberto RaulBarleyChloroplastsMutantsCebadaCloroplastoMutantesBarley Chloroplast MutatorMsh1 genePlastomeMutante del Cloroplasto de CebadaGen Msh1PlastomaThe barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences.Instituto de GenéticaFil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaMDPI2022-04-06T10:08:43Z2022-04-06T10:08:43Z2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11564https://www.mdpi.com/1422-0067/23/3/18141422-0067https://doi.org/10.3390/ijms23031814International Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuarioinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:31Zoai:localhost:20.500.12123/11564instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:31.663INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
title The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
spellingShingle The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
Lencina, Franco Daniel
Barley
Chloroplasts
Mutants
Cebada
Cloroplasto
Mutantes
Barley Chloroplast Mutator
Msh1 gene
Plastome
Mutante del Cloroplasto de Cebada
Gen Msh1
Plastoma
title_short The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
title_full The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
title_fullStr The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
title_full_unstemmed The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
title_sort The barley chloroplast mutator (cpm) mutant: all roads lead to the Msh1 gene
dc.creator.none.fl_str_mv Lencina, Franco Daniel
Landau, Alejandra Mabel
Prina, Alberto Raul
author Lencina, Franco Daniel
author_facet Lencina, Franco Daniel
Landau, Alejandra Mabel
Prina, Alberto Raul
author_role author
author2 Landau, Alejandra Mabel
Prina, Alberto Raul
author2_role author
author
dc.subject.none.fl_str_mv Barley
Chloroplasts
Mutants
Cebada
Cloroplasto
Mutantes
Barley Chloroplast Mutator
Msh1 gene
Plastome
Mutante del Cloroplasto de Cebada
Gen Msh1
Plastoma
topic Barley
Chloroplasts
Mutants
Cebada
Cloroplasto
Mutantes
Barley Chloroplast Mutator
Msh1 gene
Plastome
Mutante del Cloroplasto de Cebada
Gen Msh1
Plastoma
dc.description.none.fl_txt_mv The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences.
Instituto de Genética
Fil: Lencina, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Landau, Alejandra Mabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Prina, Alberto Raul. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was established in progenies from crosses and backcrosses. These results strongly suggest that the mutation identified in the Msh1 gene of the cpm mutant is responsible for the observed plastome instabilities. Interestingly, comparison of mutant phenotypes and molecular changes induced by the barley cpm mutant with those of Arabidopsis MSH1 mutants revealed marked differences.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-06T10:08:43Z
2022-04-06T10:08:43Z
2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11564
https://www.mdpi.com/1422-0067/23/3/1814
1422-0067
https://doi.org/10.3390/ijms23031814
url http://hdl.handle.net/20.500.12123/11564
https://www.mdpi.com/1422-0067/23/3/1814
https://doi.org/10.3390/ijms23031814
identifier_str_mv 1422-0067
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131024/AR./Desarrollo de sistemas alternativos de generación y utilización de variabilidad genética y su aplicación al mejoramiento de los cultivos.
info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv International Journal of Molecular Sciences 23 (3) : 1814 (Febrero 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619163356626944
score 12.558318

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