Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels

Autores
Valenzuela Alvarez, Matias; Matos, Bruno; Correa, Alejandro; Bolontrade, Marcela Fabiana
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Osteosarcoma (OS) is the most frequent bone tumor in pediatrics and presents two critical clinical challenges, metastasis and chemoresistance. Better diagnostic and prognostic tools for OS disease progression are in need. Here we propose the use of micro-RNAs (miRNAs) as alternative diagnostic biomarkers for OS. MiRNAs are small and stable non-coding RNAs that can be obtained from liquid biopsies of different body fluids such as plasma, that in the last years have been proposed as diagnostic and prognostic biomarkers. The aim of this work was to assess an OS miRNAs database and contrast it with our own molecular and functional profiling in an OS model with metastatic behavior, in order to propose possible miRNAs as biomarker candidates. We analyzed circulating miRNAs present in the plasma of 15 healthy donors and 20 OS patients (10 with localized OS and 10 with metastatic OS) using the miRNAs dataset GSE65071. Our analysis revealed that miR-34a-5p, -200a-3p, -582-5p, -624-5p and let-7a-3p were upregulated in OS patients plasma as compared to healthy donors (fold change: 0.43; 0.78; 0.78; 0.95; 0.5 respectively; p < 0.0001), while miR-27a-3p and -221-3p were found downregulated in the plasma of OS patients as compared to healthy donors (fold change: -0.73; -2.64 respectively; p < 0.0001). There was no difference in expression between localized and metastatic OS for these miRNAs. Bioinformatics analysis of the target genes for these miRNAs revealed that they are implicated in the regulation of different cancer-related biological pathways like ECM- receptor interaction, cell cycle control and EMT, in coincidence with our proteomic approach on metastatic and non-metastatic OS cells. These results strengthen the in-silico search and constitute a proof of concept on the use of this cross-omic approach as a tool for the identification of potential miRNAs as liquid biopsy biomarkers for diseases characterized by scarce extensive population-based data.
Fil: Valenzuela Alvarez, Matias. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; Argentina
Fil: Matos, Bruno. No especifíca;
Fil: Correa, Alejandro. No especifíca;
Fil: Bolontrade, Marcela Fabiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
OSTEOSARCOMA
CIRCULATING MIRNA
CHEMORESISTANCE
POTENTIAL BIOMARKERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/154348
Acceder
id CONICETDig_c3485375da3db6eda91168328a8c8343
oai_identifier_str oai:ri.conicet.gov.ar:11336/154348
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panelsValenzuela Alvarez, MatiasMatos, BrunoCorrea, AlejandroBolontrade, Marcela FabianaOSTEOSARCOMACIRCULATING MIRNACHEMORESISTANCEPOTENTIAL BIOMARKERShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Osteosarcoma (OS) is the most frequent bone tumor in pediatrics and presents two critical clinical challenges, metastasis and chemoresistance. Better diagnostic and prognostic tools for OS disease progression are in need. Here we propose the use of micro-RNAs (miRNAs) as alternative diagnostic biomarkers for OS. MiRNAs are small and stable non-coding RNAs that can be obtained from liquid biopsies of different body fluids such as plasma, that in the last years have been proposed as diagnostic and prognostic biomarkers. The aim of this work was to assess an OS miRNAs database and contrast it with our own molecular and functional profiling in an OS model with metastatic behavior, in order to propose possible miRNAs as biomarker candidates. We analyzed circulating miRNAs present in the plasma of 15 healthy donors and 20 OS patients (10 with localized OS and 10 with metastatic OS) using the miRNAs dataset GSE65071. Our analysis revealed that miR-34a-5p, -200a-3p, -582-5p, -624-5p and let-7a-3p were upregulated in OS patients plasma as compared to healthy donors (fold change: 0.43; 0.78; 0.78; 0.95; 0.5 respectively; p < 0.0001), while miR-27a-3p and -221-3p were found downregulated in the plasma of OS patients as compared to healthy donors (fold change: -0.73; -2.64 respectively; p < 0.0001). There was no difference in expression between localized and metastatic OS for these miRNAs. Bioinformatics analysis of the target genes for these miRNAs revealed that they are implicated in the regulation of different cancer-related biological pathways like ECM- receptor interaction, cell cycle control and EMT, in coincidence with our proteomic approach on metastatic and non-metastatic OS cells. These results strengthen the in-silico search and constitute a proof of concept on the use of this cross-omic approach as a tool for the identification of potential miRNAs as liquid biopsy biomarkers for diseases characterized by scarce extensive population-based data.Fil: Valenzuela Alvarez, Matias. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; ArgentinaFil: Matos, Bruno. No especifíca;Fil: Correa, Alejandro. No especifíca;Fil: Bolontrade, Marcela Fabiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; ArgentinaLXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS)ArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/154348Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); Argentina; 2020; 1-50025-7680CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://inmunologia.org.ar/revista-medicina-2020/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:03Zoai:ri.conicet.gov.ar:11336/154348instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:03.864CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
title Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
spellingShingle Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
Valenzuela Alvarez, Matias
OSTEOSARCOMA
CIRCULATING MIRNA
CHEMORESISTANCE
POTENTIAL BIOMARKERS
title_short Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
title_full Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
title_fullStr Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
title_full_unstemmed Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
title_sort Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels
dc.creator.none.fl_str_mv Valenzuela Alvarez, Matias
Matos, Bruno
Correa, Alejandro
Bolontrade, Marcela Fabiana
author Valenzuela Alvarez, Matias
author_facet Valenzuela Alvarez, Matias
Matos, Bruno
Correa, Alejandro
Bolontrade, Marcela Fabiana
author_role author
author2 Matos, Bruno
Correa, Alejandro
Bolontrade, Marcela Fabiana
author2_role author
author
author
dc.subject.none.fl_str_mv OSTEOSARCOMA
CIRCULATING MIRNA
CHEMORESISTANCE
POTENTIAL BIOMARKERS
topic OSTEOSARCOMA
CIRCULATING MIRNA
CHEMORESISTANCE
POTENTIAL BIOMARKERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Osteosarcoma (OS) is the most frequent bone tumor in pediatrics and presents two critical clinical challenges, metastasis and chemoresistance. Better diagnostic and prognostic tools for OS disease progression are in need. Here we propose the use of micro-RNAs (miRNAs) as alternative diagnostic biomarkers for OS. MiRNAs are small and stable non-coding RNAs that can be obtained from liquid biopsies of different body fluids such as plasma, that in the last years have been proposed as diagnostic and prognostic biomarkers. The aim of this work was to assess an OS miRNAs database and contrast it with our own molecular and functional profiling in an OS model with metastatic behavior, in order to propose possible miRNAs as biomarker candidates. We analyzed circulating miRNAs present in the plasma of 15 healthy donors and 20 OS patients (10 with localized OS and 10 with metastatic OS) using the miRNAs dataset GSE65071. Our analysis revealed that miR-34a-5p, -200a-3p, -582-5p, -624-5p and let-7a-3p were upregulated in OS patients plasma as compared to healthy donors (fold change: 0.43; 0.78; 0.78; 0.95; 0.5 respectively; p < 0.0001), while miR-27a-3p and -221-3p were found downregulated in the plasma of OS patients as compared to healthy donors (fold change: -0.73; -2.64 respectively; p < 0.0001). There was no difference in expression between localized and metastatic OS for these miRNAs. Bioinformatics analysis of the target genes for these miRNAs revealed that they are implicated in the regulation of different cancer-related biological pathways like ECM- receptor interaction, cell cycle control and EMT, in coincidence with our proteomic approach on metastatic and non-metastatic OS cells. These results strengthen the in-silico search and constitute a proof of concept on the use of this cross-omic approach as a tool for the identification of potential miRNAs as liquid biopsy biomarkers for diseases characterized by scarce extensive population-based data.
Fil: Valenzuela Alvarez, Matias. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; Argentina
Fil: Matos, Bruno. No especifíca;
Fil: Correa, Alejandro. No especifíca;
Fil: Bolontrade, Marcela Fabiana. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Hospital Italiano. Instituto de Medicina Traslacional E Ingenieria Biomedica. - Instituto Universitario Hospital Italiano de Buenos Aires. Instituto de Medicina Traslacional E Ingenieria Biomedica.; Argentina
LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS)
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Osteosarcoma (OS) is the most frequent bone tumor in pediatrics and presents two critical clinical challenges, metastasis and chemoresistance. Better diagnostic and prognostic tools for OS disease progression are in need. Here we propose the use of micro-RNAs (miRNAs) as alternative diagnostic biomarkers for OS. MiRNAs are small and stable non-coding RNAs that can be obtained from liquid biopsies of different body fluids such as plasma, that in the last years have been proposed as diagnostic and prognostic biomarkers. The aim of this work was to assess an OS miRNAs database and contrast it with our own molecular and functional profiling in an OS model with metastatic behavior, in order to propose possible miRNAs as biomarker candidates. We analyzed circulating miRNAs present in the plasma of 15 healthy donors and 20 OS patients (10 with localized OS and 10 with metastatic OS) using the miRNAs dataset GSE65071. Our analysis revealed that miR-34a-5p, -200a-3p, -582-5p, -624-5p and let-7a-3p were upregulated in OS patients plasma as compared to healthy donors (fold change: 0.43; 0.78; 0.78; 0.95; 0.5 respectively; p < 0.0001), while miR-27a-3p and -221-3p were found downregulated in the plasma of OS patients as compared to healthy donors (fold change: -0.73; -2.64 respectively; p < 0.0001). There was no difference in expression between localized and metastatic OS for these miRNAs. Bioinformatics analysis of the target genes for these miRNAs revealed that they are implicated in the regulation of different cancer-related biological pathways like ECM- receptor interaction, cell cycle control and EMT, in coincidence with our proteomic approach on metastatic and non-metastatic OS cells. These results strengthen the in-silico search and constitute a proof of concept on the use of this cross-omic approach as a tool for the identification of potential miRNAs as liquid biopsy biomarkers for diseases characterized by scarce extensive population-based data.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/154348
Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); Argentina; 2020; 1-5
0025-7680
CONICET Digital
CONICET
url http://hdl.handle.net/11336/154348
identifier_str_mv Osteosarcoma and miRNAs: combining in silico miRNA analysis and proteomic profiling in search of potential diagnostic panels; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXVIII Reunión Anual de la Sociedad Argentina de Inmunología (SAI), Reunión Anual de la Sociedad Argentina de Fisiología (SAFIS); Argentina; 2020; 1-5
0025-7680
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://inmunologia.org.ar/revista-medicina-2020/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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