Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels

Autores
Leuner, Kristina; Li, Wei; Amaral, Michelle D.; Rudolph, Stephanie; Calfa, Gaston Diego; Schuwald, Anita M.; Harteneck, Christian; Inoue, Takafumi; Pozzo Miller, Lucas
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The standardized extract of the St. John's wort plant (Hypericum perforatum) is commonly used to treat mild to moderate depression. Its active constituent is hyperforin, a phloroglucinol derivative that reduces the reuptake of serotonin and norepinephrine by increasing intracellular Na+ concentration through the activation of nonselective cationic TRPC6 channels. TRPC6 channels are also Ca2+-permeable, resulting in intracellular Ca2+ elevations. Indeed, hyperforin activates TRPC6-mediated currents and Ca2+ transients in rat PC12 cells, which induce their differentiation, mimicking the neurotrophic effect of nerve growth factor. Here, we show that hyperforin modulates dendritic spine morphology in CA1 and CA3 pyramidal neurons of hippocampal slice cultures through the activation of TRPC6 channels. Hyperforin also evoked intracellular Ca2+ transients and depolarizing inward currents sensitive to the TRPC channel blocker La3+, thus resembling the actions of the neurotrophin brain-derived neurotrophic factor (BDNF) in hippocampal pyramidal neurons. These results suggest that the antidepressant actions of St. John's wort are mediated by a mechanism similar to that engaged by BDNF.
Fil: Leuner, Kristina. University of Alabama at Birmingahm; Estados Unidos. Goethe Universitat Frankfurt; Alemania
Fil: Li, Wei. University of Alabama at Birmingahm; Estados Unidos
Fil: Amaral, Michelle D.. University of Alabama at Birmingahm; Estados Unidos
Fil: Rudolph, Stephanie. University of Alabama at Birmingahm; Estados Unidos. University of Freiburg; Alemania
Fil: Calfa, Gaston Diego. University of Alabama at Birmingahm; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Schuwald, Anita M.. Goethe Universitat Frankfurt; Alemania
Fil: Harteneck, Christian. Eberhard-Karls University; Alemania
Fil: Inoue, Takafumi. Waseda University; Japón
Fil: Pozzo Miller, Lucas. University of Alabama at Birmingahm; Estados Unidos
Materia
Hippocampus
Trpc6
Sirna
Organotipic Slices
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24943
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24943
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channelsLeuner, KristinaLi, WeiAmaral, Michelle D.Rudolph, StephanieCalfa, Gaston DiegoSchuwald, Anita M.Harteneck, ChristianInoue, TakafumiPozzo Miller, LucasHippocampusTrpc6SirnaOrganotipic Sliceshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The standardized extract of the St. John's wort plant (Hypericum perforatum) is commonly used to treat mild to moderate depression. Its active constituent is hyperforin, a phloroglucinol derivative that reduces the reuptake of serotonin and norepinephrine by increasing intracellular Na+ concentration through the activation of nonselective cationic TRPC6 channels. TRPC6 channels are also Ca2+-permeable, resulting in intracellular Ca2+ elevations. Indeed, hyperforin activates TRPC6-mediated currents and Ca2+ transients in rat PC12 cells, which induce their differentiation, mimicking the neurotrophic effect of nerve growth factor. Here, we show that hyperforin modulates dendritic spine morphology in CA1 and CA3 pyramidal neurons of hippocampal slice cultures through the activation of TRPC6 channels. Hyperforin also evoked intracellular Ca2+ transients and depolarizing inward currents sensitive to the TRPC channel blocker La3+, thus resembling the actions of the neurotrophin brain-derived neurotrophic factor (BDNF) in hippocampal pyramidal neurons. These results suggest that the antidepressant actions of St. John's wort are mediated by a mechanism similar to that engaged by BDNF.Fil: Leuner, Kristina. University of Alabama at Birmingahm; Estados Unidos. Goethe Universitat Frankfurt; AlemaniaFil: Li, Wei. University of Alabama at Birmingahm; Estados UnidosFil: Amaral, Michelle D.. University of Alabama at Birmingahm; Estados UnidosFil: Rudolph, Stephanie. University of Alabama at Birmingahm; Estados Unidos. University of Freiburg; AlemaniaFil: Calfa, Gaston Diego. University of Alabama at Birmingahm; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Schuwald, Anita M.. Goethe Universitat Frankfurt; AlemaniaFil: Harteneck, Christian. Eberhard-Karls University; AlemaniaFil: Inoue, Takafumi. Waseda University; JapónFil: Pozzo Miller, Lucas. University of Alabama at Birmingahm; Estados UnidosWiley2012-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24943Leuner, Kristina; Li, Wei; Amaral, Michelle D.; Rudolph, Stephanie; Calfa, Gaston Diego; et al.; Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels; Wiley; Hippocampus; 23; 1; 7-2012; 40-521050-9631CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22052info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22052/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:04:32Zoai:ri.conicet.gov.ar:11336/24943instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:04:32.649CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
title Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
spellingShingle Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
Leuner, Kristina
Hippocampus
Trpc6
Sirna
Organotipic Slices
title_short Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
title_full Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
title_fullStr Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
title_full_unstemmed Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
title_sort Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels
dc.creator.none.fl_str_mv Leuner, Kristina
Li, Wei
Amaral, Michelle D.
Rudolph, Stephanie
Calfa, Gaston Diego
Schuwald, Anita M.
Harteneck, Christian
Inoue, Takafumi
Pozzo Miller, Lucas
author Leuner, Kristina
author_facet Leuner, Kristina
Li, Wei
Amaral, Michelle D.
Rudolph, Stephanie
Calfa, Gaston Diego
Schuwald, Anita M.
Harteneck, Christian
Inoue, Takafumi
Pozzo Miller, Lucas
author_role author
author2 Li, Wei
Amaral, Michelle D.
Rudolph, Stephanie
Calfa, Gaston Diego
Schuwald, Anita M.
Harteneck, Christian
Inoue, Takafumi
Pozzo Miller, Lucas
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hippocampus
Trpc6
Sirna
Organotipic Slices
topic Hippocampus
Trpc6
Sirna
Organotipic Slices
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The standardized extract of the St. John's wort plant (Hypericum perforatum) is commonly used to treat mild to moderate depression. Its active constituent is hyperforin, a phloroglucinol derivative that reduces the reuptake of serotonin and norepinephrine by increasing intracellular Na+ concentration through the activation of nonselective cationic TRPC6 channels. TRPC6 channels are also Ca2+-permeable, resulting in intracellular Ca2+ elevations. Indeed, hyperforin activates TRPC6-mediated currents and Ca2+ transients in rat PC12 cells, which induce their differentiation, mimicking the neurotrophic effect of nerve growth factor. Here, we show that hyperforin modulates dendritic spine morphology in CA1 and CA3 pyramidal neurons of hippocampal slice cultures through the activation of TRPC6 channels. Hyperforin also evoked intracellular Ca2+ transients and depolarizing inward currents sensitive to the TRPC channel blocker La3+, thus resembling the actions of the neurotrophin brain-derived neurotrophic factor (BDNF) in hippocampal pyramidal neurons. These results suggest that the antidepressant actions of St. John's wort are mediated by a mechanism similar to that engaged by BDNF.
Fil: Leuner, Kristina. University of Alabama at Birmingahm; Estados Unidos. Goethe Universitat Frankfurt; Alemania
Fil: Li, Wei. University of Alabama at Birmingahm; Estados Unidos
Fil: Amaral, Michelle D.. University of Alabama at Birmingahm; Estados Unidos
Fil: Rudolph, Stephanie. University of Alabama at Birmingahm; Estados Unidos. University of Freiburg; Alemania
Fil: Calfa, Gaston Diego. University of Alabama at Birmingahm; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Schuwald, Anita M.. Goethe Universitat Frankfurt; Alemania
Fil: Harteneck, Christian. Eberhard-Karls University; Alemania
Fil: Inoue, Takafumi. Waseda University; Japón
Fil: Pozzo Miller, Lucas. University of Alabama at Birmingahm; Estados Unidos
description The standardized extract of the St. John's wort plant (Hypericum perforatum) is commonly used to treat mild to moderate depression. Its active constituent is hyperforin, a phloroglucinol derivative that reduces the reuptake of serotonin and norepinephrine by increasing intracellular Na+ concentration through the activation of nonselective cationic TRPC6 channels. TRPC6 channels are also Ca2+-permeable, resulting in intracellular Ca2+ elevations. Indeed, hyperforin activates TRPC6-mediated currents and Ca2+ transients in rat PC12 cells, which induce their differentiation, mimicking the neurotrophic effect of nerve growth factor. Here, we show that hyperforin modulates dendritic spine morphology in CA1 and CA3 pyramidal neurons of hippocampal slice cultures through the activation of TRPC6 channels. Hyperforin also evoked intracellular Ca2+ transients and depolarizing inward currents sensitive to the TRPC channel blocker La3+, thus resembling the actions of the neurotrophin brain-derived neurotrophic factor (BDNF) in hippocampal pyramidal neurons. These results suggest that the antidepressant actions of St. John's wort are mediated by a mechanism similar to that engaged by BDNF.
publishDate 2012
dc.date.none.fl_str_mv 2012-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24943
Leuner, Kristina; Li, Wei; Amaral, Michelle D.; Rudolph, Stephanie; Calfa, Gaston Diego; et al.; Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels; Wiley; Hippocampus; 23; 1; 7-2012; 40-52
1050-9631
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24943
identifier_str_mv Leuner, Kristina; Li, Wei; Amaral, Michelle D.; Rudolph, Stephanie; Calfa, Gaston Diego; et al.; Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca2+- permeable TRPC6 channels; Wiley; Hippocampus; 23; 1; 7-2012; 40-52
1050-9631
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22052
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22052/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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