Lipid-independent control of endothelial and neuronal TRPC3 channels by light

Autores
Tiapko, Oleksandra; Shrestha, Niroj; Lindinger, Sonja; Guedes De La Cruz, Gema; Graziani, Annarita; Klec, Christiane; Butorac, Carmen; Graier, Wolfgang. F.; Kubista, Helmut; Freichel, Marc; Birnbaumer, Lutz; Romanin, Christoph; Glasnov, Toma; Groschner, Klaus
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that enables manipulation of TRPC3 channels by light, in a manner independent of lipid metabolism and with higher temporal precision than lipid photopharmacology. Using the azobenzene photoswitch moiety, we modified GSK1702934A to generate light-controlled TRPC agonists. We obtained one light-sensitive molecule (OptoBI-1) that allows us to exert efficient, light-mediated control over TRPC3 activity and the associated cellular Ca2+ signaling. OptoBI-1 enabled high-precision, temporal control of TRPC3-linked cell functions such as neuronal firing and endothelial Ca2+ transients. With these findings, we introduce a novel photopharmacological strategy to control native TRPC conductances.
Fil: Tiapko, Oleksandra. Medical University of Graz; Austria
Fil: Shrestha, Niroj. Medical University of Graz; Austria
Fil: Lindinger, Sonja. University Linz; Austria
Fil: Guedes De La Cruz, Gema. University Of Graz.; Austria
Fil: Graziani, Annarita. Medical University of Graz; Austria
Fil: Klec, Christiane. University Of Graz.; Austria
Fil: Butorac, Carmen. University Linz; Austria
Fil: Graier, Wolfgang. F.. University Of Graz.; Austria
Fil: Kubista, Helmut. No especifíca;
Fil: Freichel, Marc. Universität Heidelberg; Alemania
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Romanin, Christoph. University Linz; Austria
Fil: Glasnov, Toma. Graz University Of Technology.; Austria
Fil: Groschner, Klaus. Graz University Of Technology.; Austria
Materia
TRPC3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120566
Acceder
id CONICETDig_d25279237d2e587a508249dd43013789
oai_identifier_str oai:ri.conicet.gov.ar:11336/120566
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lipid-independent control of endothelial and neuronal TRPC3 channels by lightTiapko, OleksandraShrestha, NirojLindinger, SonjaGuedes De La Cruz, GemaGraziani, AnnaritaKlec, ChristianeButorac, CarmenGraier, Wolfgang. F.Kubista, HelmutFreichel, MarcBirnbaumer, LutzRomanin, ChristophGlasnov, TomaGroschner, KlausTRPC3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that enables manipulation of TRPC3 channels by light, in a manner independent of lipid metabolism and with higher temporal precision than lipid photopharmacology. Using the azobenzene photoswitch moiety, we modified GSK1702934A to generate light-controlled TRPC agonists. We obtained one light-sensitive molecule (OptoBI-1) that allows us to exert efficient, light-mediated control over TRPC3 activity and the associated cellular Ca2+ signaling. OptoBI-1 enabled high-precision, temporal control of TRPC3-linked cell functions such as neuronal firing and endothelial Ca2+ transients. With these findings, we introduce a novel photopharmacological strategy to control native TRPC conductances.Fil: Tiapko, Oleksandra. Medical University of Graz; AustriaFil: Shrestha, Niroj. Medical University of Graz; AustriaFil: Lindinger, Sonja. University Linz; AustriaFil: Guedes De La Cruz, Gema. University Of Graz.; AustriaFil: Graziani, Annarita. Medical University of Graz; AustriaFil: Klec, Christiane. University Of Graz.; AustriaFil: Butorac, Carmen. University Linz; AustriaFil: Graier, Wolfgang. F.. University Of Graz.; AustriaFil: Kubista, Helmut. No especifíca;Fil: Freichel, Marc. Universität Heidelberg; AlemaniaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Romanin, Christoph. University Linz; AustriaFil: Glasnov, Toma. Graz University Of Technology.; AustriaFil: Groschner, Klaus. Graz University Of Technology.; AustriaRoyal Society of Chemistry2019-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120566Tiapko, Oleksandra; Shrestha, Niroj; Lindinger, Sonja; Guedes De La Cruz, Gema; Graziani, Annarita; et al.; Lipid-independent control of endothelial and neuronal TRPC3 channels by light; Royal Society of Chemistry; Chemical Science; 10; 9; 1-2019; 2837-28422041-65202041-6539CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c8sc05536jinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:03:07Zoai:ri.conicet.gov.ar:11336/120566instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:03:07.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title Lipid-independent control of endothelial and neuronal TRPC3 channels by light
spellingShingle Lipid-independent control of endothelial and neuronal TRPC3 channels by light
Tiapko, Oleksandra
TRPC3
title_short Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_full Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_fullStr Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_full_unstemmed Lipid-independent control of endothelial and neuronal TRPC3 channels by light
title_sort Lipid-independent control of endothelial and neuronal TRPC3 channels by light
dc.creator.none.fl_str_mv Tiapko, Oleksandra
Shrestha, Niroj
Lindinger, Sonja
Guedes De La Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang. F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
author Tiapko, Oleksandra
author_facet Tiapko, Oleksandra
Shrestha, Niroj
Lindinger, Sonja
Guedes De La Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang. F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
author_role author
author2 Shrestha, Niroj
Lindinger, Sonja
Guedes De La Cruz, Gema
Graziani, Annarita
Klec, Christiane
Butorac, Carmen
Graier, Wolfgang. F.
Kubista, Helmut
Freichel, Marc
Birnbaumer, Lutz
Romanin, Christoph
Glasnov, Toma
Groschner, Klaus
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRPC3
topic TRPC3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that enables manipulation of TRPC3 channels by light, in a manner independent of lipid metabolism and with higher temporal precision than lipid photopharmacology. Using the azobenzene photoswitch moiety, we modified GSK1702934A to generate light-controlled TRPC agonists. We obtained one light-sensitive molecule (OptoBI-1) that allows us to exert efficient, light-mediated control over TRPC3 activity and the associated cellular Ca2+ signaling. OptoBI-1 enabled high-precision, temporal control of TRPC3-linked cell functions such as neuronal firing and endothelial Ca2+ transients. With these findings, we introduce a novel photopharmacological strategy to control native TRPC conductances.
Fil: Tiapko, Oleksandra. Medical University of Graz; Austria
Fil: Shrestha, Niroj. Medical University of Graz; Austria
Fil: Lindinger, Sonja. University Linz; Austria
Fil: Guedes De La Cruz, Gema. University Of Graz.; Austria
Fil: Graziani, Annarita. Medical University of Graz; Austria
Fil: Klec, Christiane. University Of Graz.; Austria
Fil: Butorac, Carmen. University Linz; Austria
Fil: Graier, Wolfgang. F.. University Of Graz.; Austria
Fil: Kubista, Helmut. No especifíca;
Fil: Freichel, Marc. Universität Heidelberg; Alemania
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Romanin, Christoph. University Linz; Austria
Fil: Glasnov, Toma. Graz University Of Technology.; Austria
Fil: Groschner, Klaus. Graz University Of Technology.; Austria
description Lipid-gated TRPC channels are highly expressed in cardiovascular and neuronal tissues. Exerting precise pharmacological control over their activity in native cells is expected to serve as a basis for the development of novel therapies. Here we report on a new photopharmacological tool that enables manipulation of TRPC3 channels by light, in a manner independent of lipid metabolism and with higher temporal precision than lipid photopharmacology. Using the azobenzene photoswitch moiety, we modified GSK1702934A to generate light-controlled TRPC agonists. We obtained one light-sensitive molecule (OptoBI-1) that allows us to exert efficient, light-mediated control over TRPC3 activity and the associated cellular Ca2+ signaling. OptoBI-1 enabled high-precision, temporal control of TRPC3-linked cell functions such as neuronal firing and endothelial Ca2+ transients. With these findings, we introduce a novel photopharmacological strategy to control native TRPC conductances.
publishDate 2019
dc.date.none.fl_str_mv 2019-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120566
Tiapko, Oleksandra; Shrestha, Niroj; Lindinger, Sonja; Guedes De La Cruz, Gema; Graziani, Annarita; et al.; Lipid-independent control of endothelial and neuronal TRPC3 channels by light; Royal Society of Chemistry; Chemical Science; 10; 9; 1-2019; 2837-2842
2041-6520
2041-6539
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120566
identifier_str_mv Tiapko, Oleksandra; Shrestha, Niroj; Lindinger, Sonja; Guedes De La Cruz, Gema; Graziani, Annarita; et al.; Lipid-independent control of endothelial and neuronal TRPC3 channels by light; Royal Society of Chemistry; Chemical Science; 10; 9; 1-2019; 2837-2842
2041-6520
2041-6539
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c8sc05536j
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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