Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3

Autores
Aispuru, Gualberto Rodrigo; Aguirre, María Victoria; Aquino Esperanza, José Andrés; Lettieri, Carolina Noelia; Juaristi, Julian Antonio; Brandan, Nora Cristina
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Erythropoietic stress occurs under conditions of tissular hypoxia, such as anemia. Functional relationships between erythroid bone marrow (BM) proliferation, differentiation, the expression of survival and apoptotic related proteins, as well as the features of the BM microenvironment upon acute anemic stress, are not fully elucidated. To achieve this aim, CF-1 Swiss mice were injected with a single dose of 5-fluorouracil (5-FU, 150 mg/kg ip) and a multiparametric analysis was conducted for 20 days. Apoptosis (TUNEL assay), BM architecture organization (scanning electronic microscopy), proliferation (DNA assay), differentiation (clonogenic cultures), expression of survival erythroid related proteins (EPO-R, GATA-1, Bcl-xL) as well as the expression of apoptotic- related proteins (Bax, activated Caspase-3) by Western blotting, were evaluated. Experimental data showed that apoptosis, arrest of cell proliferation and disruptions of BM architecture were maximal within the first period of acute stress (1-3 days). Bax and caspase-3 overexpressions were also coincident during this acute period. Moreover, from day 5 upon drug challenge BM responds to acute stress through the EPO-EPO-R system, prompting expressions of GATA-1 and Bcl-xL. Erythroid proliferation rates and red-cell-committed progenitors enhanced in a coordinated way to restore the size and function of the red cell compartment. A second overexpression wave of active caspase-3 was noticed during stress recovery. Together, these results indicate that in response to acute stress a dramatic increase in CFU-E (erythroid colony forming units) population is concomitant with upregulation of EPO-R, GATA-1 and Bcl-xL in the BM erythroid compartment, and that these concurrent processes are crucial for acquiring proper erythroid cell functionality without delayed response to tissular hypoxia. © 2008 International Federation for Cell Biology.
Fil: Aispuru, Gualberto Rodrigo. Universidad Nacional del Nordeste; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste; Argentina
Fil: Aquino Esperanza, José Andrés. Universidad Nacional del Nordeste; Argentina
Fil: Lettieri, Carolina Noelia. Universidad Nacional del Nordeste; Argentina
Fil: Juaristi, Julian Antonio. Universidad Nacional del Nordeste; Argentina
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
Materia
Bcl-Xl
Caspase-3
Erythropoietic Stress
Erythropoietin Receptor
Gata-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60628
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3Aispuru, Gualberto RodrigoAguirre, María VictoriaAquino Esperanza, José AndrésLettieri, Carolina NoeliaJuaristi, Julian AntonioBrandan, Nora CristinaBcl-XlCaspase-3Erythropoietic StressErythropoietin ReceptorGata-1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Erythropoietic stress occurs under conditions of tissular hypoxia, such as anemia. Functional relationships between erythroid bone marrow (BM) proliferation, differentiation, the expression of survival and apoptotic related proteins, as well as the features of the BM microenvironment upon acute anemic stress, are not fully elucidated. To achieve this aim, CF-1 Swiss mice were injected with a single dose of 5-fluorouracil (5-FU, 150 mg/kg ip) and a multiparametric analysis was conducted for 20 days. Apoptosis (TUNEL assay), BM architecture organization (scanning electronic microscopy), proliferation (DNA assay), differentiation (clonogenic cultures), expression of survival erythroid related proteins (EPO-R, GATA-1, Bcl-xL) as well as the expression of apoptotic- related proteins (Bax, activated Caspase-3) by Western blotting, were evaluated. Experimental data showed that apoptosis, arrest of cell proliferation and disruptions of BM architecture were maximal within the first period of acute stress (1-3 days). Bax and caspase-3 overexpressions were also coincident during this acute period. Moreover, from day 5 upon drug challenge BM responds to acute stress through the EPO-EPO-R system, prompting expressions of GATA-1 and Bcl-xL. Erythroid proliferation rates and red-cell-committed progenitors enhanced in a coordinated way to restore the size and function of the red cell compartment. A second overexpression wave of active caspase-3 was noticed during stress recovery. Together, these results indicate that in response to acute stress a dramatic increase in CFU-E (erythroid colony forming units) population is concomitant with upregulation of EPO-R, GATA-1 and Bcl-xL in the BM erythroid compartment, and that these concurrent processes are crucial for acquiring proper erythroid cell functionality without delayed response to tissular hypoxia. © 2008 International Federation for Cell Biology.Fil: Aispuru, Gualberto Rodrigo. Universidad Nacional del Nordeste; ArgentinaFil: Aguirre, María Victoria. Universidad Nacional del Nordeste; ArgentinaFil: Aquino Esperanza, José Andrés. Universidad Nacional del Nordeste; ArgentinaFil: Lettieri, Carolina Noelia. Universidad Nacional del Nordeste; ArgentinaFil: Juaristi, Julian Antonio. Universidad Nacional del Nordeste; ArgentinaFil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2008-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60628Aispuru, Gualberto Rodrigo; Aguirre, María Victoria; Aquino Esperanza, José Andrés; Lettieri, Carolina Noelia; Juaristi, Julian Antonio; et al.; Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 32; 8; 8-2008; 966-9781065-69951095-8355CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2008.04.014info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2008.04.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:59Zoai:ri.conicet.gov.ar:11336/60628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:59.82CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
title Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
spellingShingle Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
Aispuru, Gualberto Rodrigo
Bcl-Xl
Caspase-3
Erythropoietic Stress
Erythropoietin Receptor
Gata-1
title_short Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
title_full Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
title_fullStr Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
title_full_unstemmed Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
title_sort Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3
dc.creator.none.fl_str_mv Aispuru, Gualberto Rodrigo
Aguirre, María Victoria
Aquino Esperanza, José Andrés
Lettieri, Carolina Noelia
Juaristi, Julian Antonio
Brandan, Nora Cristina
author Aispuru, Gualberto Rodrigo
author_facet Aispuru, Gualberto Rodrigo
Aguirre, María Victoria
Aquino Esperanza, José Andrés
Lettieri, Carolina Noelia
Juaristi, Julian Antonio
Brandan, Nora Cristina
author_role author
author2 Aguirre, María Victoria
Aquino Esperanza, José Andrés
Lettieri, Carolina Noelia
Juaristi, Julian Antonio
Brandan, Nora Cristina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bcl-Xl
Caspase-3
Erythropoietic Stress
Erythropoietin Receptor
Gata-1
topic Bcl-Xl
Caspase-3
Erythropoietic Stress
Erythropoietin Receptor
Gata-1
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Erythropoietic stress occurs under conditions of tissular hypoxia, such as anemia. Functional relationships between erythroid bone marrow (BM) proliferation, differentiation, the expression of survival and apoptotic related proteins, as well as the features of the BM microenvironment upon acute anemic stress, are not fully elucidated. To achieve this aim, CF-1 Swiss mice were injected with a single dose of 5-fluorouracil (5-FU, 150 mg/kg ip) and a multiparametric analysis was conducted for 20 days. Apoptosis (TUNEL assay), BM architecture organization (scanning electronic microscopy), proliferation (DNA assay), differentiation (clonogenic cultures), expression of survival erythroid related proteins (EPO-R, GATA-1, Bcl-xL) as well as the expression of apoptotic- related proteins (Bax, activated Caspase-3) by Western blotting, were evaluated. Experimental data showed that apoptosis, arrest of cell proliferation and disruptions of BM architecture were maximal within the first period of acute stress (1-3 days). Bax and caspase-3 overexpressions were also coincident during this acute period. Moreover, from day 5 upon drug challenge BM responds to acute stress through the EPO-EPO-R system, prompting expressions of GATA-1 and Bcl-xL. Erythroid proliferation rates and red-cell-committed progenitors enhanced in a coordinated way to restore the size and function of the red cell compartment. A second overexpression wave of active caspase-3 was noticed during stress recovery. Together, these results indicate that in response to acute stress a dramatic increase in CFU-E (erythroid colony forming units) population is concomitant with upregulation of EPO-R, GATA-1 and Bcl-xL in the BM erythroid compartment, and that these concurrent processes are crucial for acquiring proper erythroid cell functionality without delayed response to tissular hypoxia. © 2008 International Federation for Cell Biology.
Fil: Aispuru, Gualberto Rodrigo. Universidad Nacional del Nordeste; Argentina
Fil: Aguirre, María Victoria. Universidad Nacional del Nordeste; Argentina
Fil: Aquino Esperanza, José Andrés. Universidad Nacional del Nordeste; Argentina
Fil: Lettieri, Carolina Noelia. Universidad Nacional del Nordeste; Argentina
Fil: Juaristi, Julian Antonio. Universidad Nacional del Nordeste; Argentina
Fil: Brandan, Nora Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste; Argentina
description Erythropoietic stress occurs under conditions of tissular hypoxia, such as anemia. Functional relationships between erythroid bone marrow (BM) proliferation, differentiation, the expression of survival and apoptotic related proteins, as well as the features of the BM microenvironment upon acute anemic stress, are not fully elucidated. To achieve this aim, CF-1 Swiss mice were injected with a single dose of 5-fluorouracil (5-FU, 150 mg/kg ip) and a multiparametric analysis was conducted for 20 days. Apoptosis (TUNEL assay), BM architecture organization (scanning electronic microscopy), proliferation (DNA assay), differentiation (clonogenic cultures), expression of survival erythroid related proteins (EPO-R, GATA-1, Bcl-xL) as well as the expression of apoptotic- related proteins (Bax, activated Caspase-3) by Western blotting, were evaluated. Experimental data showed that apoptosis, arrest of cell proliferation and disruptions of BM architecture were maximal within the first period of acute stress (1-3 days). Bax and caspase-3 overexpressions were also coincident during this acute period. Moreover, from day 5 upon drug challenge BM responds to acute stress through the EPO-EPO-R system, prompting expressions of GATA-1 and Bcl-xL. Erythroid proliferation rates and red-cell-committed progenitors enhanced in a coordinated way to restore the size and function of the red cell compartment. A second overexpression wave of active caspase-3 was noticed during stress recovery. Together, these results indicate that in response to acute stress a dramatic increase in CFU-E (erythroid colony forming units) population is concomitant with upregulation of EPO-R, GATA-1 and Bcl-xL in the BM erythroid compartment, and that these concurrent processes are crucial for acquiring proper erythroid cell functionality without delayed response to tissular hypoxia. © 2008 International Federation for Cell Biology.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60628
Aispuru, Gualberto Rodrigo; Aguirre, María Victoria; Aquino Esperanza, José Andrés; Lettieri, Carolina Noelia; Juaristi, Julian Antonio; et al.; Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 32; 8; 8-2008; 966-978
1065-6995
1095-8355
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60628
identifier_str_mv Aispuru, Gualberto Rodrigo; Aguirre, María Victoria; Aquino Esperanza, José Andrés; Lettieri, Carolina Noelia; Juaristi, Julian Antonio; et al.; Erythroid expansion and survival in response to acute anemia stress: The role of EPO receptor, GATA-1, Bcl-xL and caspase-3; Academic Press Ltd - Elsevier Science Ltd; Cell Biology International; 32; 8; 8-2008; 966-978
1065-6995
1095-8355
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cellbi.2008.04.014
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2008.04.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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