Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep

Autores
Salvetti, Natalia Raquel; Ortega, Hugo Hector; Veiga Lopez, Almudena; Padmanabhan, Vasantha
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Prenatal testosterone (T) excess leads to reproductive dysfunctions in sheep, which include increased ovarian follicular recruitment and persistence. To test the hypothesis that follicular disruptions in T sheep stem from changes in the developmental ontogeny of ovarian proliferation and apoptotic factors, pregnant Suffolk sheep were injected twice weekly with T propionate or dihydrotestosterone propionate (DHT; a nonaromatizable androgen) from Days 30 to 90 of gestation. Changes in developmental expression of proliferating cell nuclear antigen (PCNA), BCL2, BAX, activated CASP3, and FAS/FASLG were determined at Fetal Days 90 and 140, 22wk, 10 mo, and 21 mo of age by immunocytochemisty. Prenatal T treatment induced changes in expression of proliferative and apoptotic markers in a follicle-, age-, and steroid-specific manner. Changes in BAX were evident only during fetal life and PCNA, BCL2, and CASP3 only postnatally. Prenatal T and not DHT increased PCNA and decreased BCL2 in granulosa/ theca cells of antral follicles at 10 and 21 mo but decreased CASP3 in granulosa/theca cells of antral follicles at 22wk (prepubertal) and 10 and 21 mo. Both treatments decreased BAX immunostaining in granulosa cells of Fetal Day 90primordial/ primary follicles. Neither treatment affected FAS expression at any developmental time point in any follicular compartment. Effects on BAX appear to be programmed by androgenic actions and PCNA, BCL2, and CASP3 by estrogenic actions of T. Overall, the findings demonstrate that fetal exposure to excess T disrupts the ovarian proliferation/apoptosis balance, thus providing a basis for the follicular disruptions evidenced in these females.
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Veiga Lopez, Almudena. University of Michigan; Estados Unidos
Fil: Padmanabhan, Vasantha. University of Michigan; Estados Unidos
Materia
BAX
BCL2
CASP3
PCNA
PCOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216430
Acceder
id CONICETDig_050fb09ac71e1d42e31b2a64a35e58c8
oai_identifier_str oai:ri.conicet.gov.ar:11336/216430
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheepSalvetti, Natalia RaquelOrtega, Hugo HectorVeiga Lopez, AlmudenaPadmanabhan, VasanthaBAXBCL2CASP3PCNAPCOShttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Prenatal testosterone (T) excess leads to reproductive dysfunctions in sheep, which include increased ovarian follicular recruitment and persistence. To test the hypothesis that follicular disruptions in T sheep stem from changes in the developmental ontogeny of ovarian proliferation and apoptotic factors, pregnant Suffolk sheep were injected twice weekly with T propionate or dihydrotestosterone propionate (DHT; a nonaromatizable androgen) from Days 30 to 90 of gestation. Changes in developmental expression of proliferating cell nuclear antigen (PCNA), BCL2, BAX, activated CASP3, and FAS/FASLG were determined at Fetal Days 90 and 140, 22wk, 10 mo, and 21 mo of age by immunocytochemisty. Prenatal T treatment induced changes in expression of proliferative and apoptotic markers in a follicle-, age-, and steroid-specific manner. Changes in BAX were evident only during fetal life and PCNA, BCL2, and CASP3 only postnatally. Prenatal T and not DHT increased PCNA and decreased BCL2 in granulosa/ theca cells of antral follicles at 10 and 21 mo but decreased CASP3 in granulosa/theca cells of antral follicles at 22wk (prepubertal) and 10 and 21 mo. Both treatments decreased BAX immunostaining in granulosa cells of Fetal Day 90primordial/ primary follicles. Neither treatment affected FAS expression at any developmental time point in any follicular compartment. Effects on BAX appear to be programmed by androgenic actions and PCNA, BCL2, and CASP3 by estrogenic actions of T. Overall, the findings demonstrate that fetal exposure to excess T disrupts the ovarian proliferation/apoptosis balance, thus providing a basis for the follicular disruptions evidenced in these females.Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; ArgentinaFil: Veiga Lopez, Almudena. University of Michigan; Estados UnidosFil: Padmanabhan, Vasantha. University of Michigan; Estados UnidosSociety for the Study of Reproduction2012-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216430Salvetti, Natalia Raquel; Ortega, Hugo Hector; Veiga Lopez, Almudena; Padmanabhan, Vasantha; Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep; Society for the Study of Reproduction; Biology of Reproduction; 87; 1; 3-2012; 1-100006-3363CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.112.100024info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406557/info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/87/1/22,%201-10/2514031?login=falseinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:58Zoai:ri.conicet.gov.ar:11336/216430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:58.469CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
title Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
spellingShingle Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
Salvetti, Natalia Raquel
BAX
BCL2
CASP3
PCNA
PCOS
title_short Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
title_full Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
title_fullStr Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
title_full_unstemmed Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
title_sort Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep
dc.creator.none.fl_str_mv Salvetti, Natalia Raquel
Ortega, Hugo Hector
Veiga Lopez, Almudena
Padmanabhan, Vasantha
author Salvetti, Natalia Raquel
author_facet Salvetti, Natalia Raquel
Ortega, Hugo Hector
Veiga Lopez, Almudena
Padmanabhan, Vasantha
author_role author
author2 Ortega, Hugo Hector
Veiga Lopez, Almudena
Padmanabhan, Vasantha
author2_role author
author
author
dc.subject.none.fl_str_mv BAX
BCL2
CASP3
PCNA
PCOS
topic BAX
BCL2
CASP3
PCNA
PCOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Prenatal testosterone (T) excess leads to reproductive dysfunctions in sheep, which include increased ovarian follicular recruitment and persistence. To test the hypothesis that follicular disruptions in T sheep stem from changes in the developmental ontogeny of ovarian proliferation and apoptotic factors, pregnant Suffolk sheep were injected twice weekly with T propionate or dihydrotestosterone propionate (DHT; a nonaromatizable androgen) from Days 30 to 90 of gestation. Changes in developmental expression of proliferating cell nuclear antigen (PCNA), BCL2, BAX, activated CASP3, and FAS/FASLG were determined at Fetal Days 90 and 140, 22wk, 10 mo, and 21 mo of age by immunocytochemisty. Prenatal T treatment induced changes in expression of proliferative and apoptotic markers in a follicle-, age-, and steroid-specific manner. Changes in BAX were evident only during fetal life and PCNA, BCL2, and CASP3 only postnatally. Prenatal T and not DHT increased PCNA and decreased BCL2 in granulosa/ theca cells of antral follicles at 10 and 21 mo but decreased CASP3 in granulosa/theca cells of antral follicles at 22wk (prepubertal) and 10 and 21 mo. Both treatments decreased BAX immunostaining in granulosa cells of Fetal Day 90primordial/ primary follicles. Neither treatment affected FAS expression at any developmental time point in any follicular compartment. Effects on BAX appear to be programmed by androgenic actions and PCNA, BCL2, and CASP3 by estrogenic actions of T. Overall, the findings demonstrate that fetal exposure to excess T disrupts the ovarian proliferation/apoptosis balance, thus providing a basis for the follicular disruptions evidenced in these females.
Fil: Salvetti, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral; Argentina
Fil: Veiga Lopez, Almudena. University of Michigan; Estados Unidos
Fil: Padmanabhan, Vasantha. University of Michigan; Estados Unidos
description Prenatal testosterone (T) excess leads to reproductive dysfunctions in sheep, which include increased ovarian follicular recruitment and persistence. To test the hypothesis that follicular disruptions in T sheep stem from changes in the developmental ontogeny of ovarian proliferation and apoptotic factors, pregnant Suffolk sheep were injected twice weekly with T propionate or dihydrotestosterone propionate (DHT; a nonaromatizable androgen) from Days 30 to 90 of gestation. Changes in developmental expression of proliferating cell nuclear antigen (PCNA), BCL2, BAX, activated CASP3, and FAS/FASLG were determined at Fetal Days 90 and 140, 22wk, 10 mo, and 21 mo of age by immunocytochemisty. Prenatal T treatment induced changes in expression of proliferative and apoptotic markers in a follicle-, age-, and steroid-specific manner. Changes in BAX were evident only during fetal life and PCNA, BCL2, and CASP3 only postnatally. Prenatal T and not DHT increased PCNA and decreased BCL2 in granulosa/ theca cells of antral follicles at 10 and 21 mo but decreased CASP3 in granulosa/theca cells of antral follicles at 22wk (prepubertal) and 10 and 21 mo. Both treatments decreased BAX immunostaining in granulosa cells of Fetal Day 90primordial/ primary follicles. Neither treatment affected FAS expression at any developmental time point in any follicular compartment. Effects on BAX appear to be programmed by androgenic actions and PCNA, BCL2, and CASP3 by estrogenic actions of T. Overall, the findings demonstrate that fetal exposure to excess T disrupts the ovarian proliferation/apoptosis balance, thus providing a basis for the follicular disruptions evidenced in these females.
publishDate 2012
dc.date.none.fl_str_mv 2012-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216430
Salvetti, Natalia Raquel; Ortega, Hugo Hector; Veiga Lopez, Almudena; Padmanabhan, Vasantha; Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep; Society for the Study of Reproduction; Biology of Reproduction; 87; 1; 3-2012; 1-10
0006-3363
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216430
identifier_str_mv Salvetti, Natalia Raquel; Ortega, Hugo Hector; Veiga Lopez, Almudena; Padmanabhan, Vasantha; Developmental programming: Impact of prenatal testosterone excess on ovarian cell proliferation and apoptotic factors in sheep; Society for the Study of Reproduction; Biology of Reproduction; 87; 1; 3-2012; 1-10
0006-3363
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.112.100024
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3406557/
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/87/1/22,%201-10/2514031?login=false
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for the Study of Reproduction
publisher.none.fl_str_mv Society for the Study of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269884132622336
score 13.13397

Publicaciones similares