Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor

Autores
Giordano, Pablo César; Beccaria, Alejandro José; Goicoechea, Hector Casimiro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An optimal medium to culture Chlorella sp., microalgae capable of storage intracellular lipids was obtained. This culture medium consists of a saline base plus carbon-energy and nitrogen sources. Significant factors exerting influence on the culture parameters were selected. Then, by applying response surface methodology coupled to desirability function, an optimal formulation, specific for the heterotrophic growth of Chlorella sp. that allows maximizing lipid concentration was obtained. During the experimental verification, the possibility of replacing commercial glucose by hydrolysates obtained from lignocellulosic materials was evaluated. Biochemical hydrolysate of corn bran allowed obtaining important improvements in lipid concentration. Finally, the optimal formulation was evaluated in an air-lift bioreactor performing a fed-batch culture. Culturing the strain in these conditions allowed rising lipid concentrations.
Fil: Giordano, Pablo César. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Beccaria, Alejandro José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Bioreactor Air-Lift
Chlorella Sp.
Acumulación de Lípidos
Escalado
Hidrolizados de Materiales Lignocelulósicos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31263
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactorGiordano, Pablo CésarBeccaria, Alejandro JoséGoicoechea, Hector CasimiroBioreactor Air-LiftChlorella Sp.Acumulación de LípidosEscaladoHidrolizados de Materiales Lignocelulósicoshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1An optimal medium to culture Chlorella sp., microalgae capable of storage intracellular lipids was obtained. This culture medium consists of a saline base plus carbon-energy and nitrogen sources. Significant factors exerting influence on the culture parameters were selected. Then, by applying response surface methodology coupled to desirability function, an optimal formulation, specific for the heterotrophic growth of Chlorella sp. that allows maximizing lipid concentration was obtained. During the experimental verification, the possibility of replacing commercial glucose by hydrolysates obtained from lignocellulosic materials was evaluated. Biochemical hydrolysate of corn bran allowed obtaining important improvements in lipid concentration. Finally, the optimal formulation was evaluated in an air-lift bioreactor performing a fed-batch culture. Culturing the strain in these conditions allowed rising lipid concentrations.Fil: Giordano, Pablo César. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Beccaria, Alejandro José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaElsevier2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31263Goicoechea, Hector Casimiro; Beccaria, Alejandro José; Giordano, Pablo César; Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor; Elsevier; Bioresource Technology; 158; 2-2014; 266-2770960-8524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852414002089info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2014.02.037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:50Zoai:ri.conicet.gov.ar:11336/31263instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:50.921CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
title Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
spellingShingle Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
Giordano, Pablo César
Bioreactor Air-Lift
Chlorella Sp.
Acumulación de Lípidos
Escalado
Hidrolizados de Materiales Lignocelulósicos
title_short Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
title_full Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
title_fullStr Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
title_full_unstemmed Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
title_sort Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor
dc.creator.none.fl_str_mv Giordano, Pablo César
Beccaria, Alejandro José
Goicoechea, Hector Casimiro
author Giordano, Pablo César
author_facet Giordano, Pablo César
Beccaria, Alejandro José
Goicoechea, Hector Casimiro
author_role author
author2 Beccaria, Alejandro José
Goicoechea, Hector Casimiro
author2_role author
author
dc.subject.none.fl_str_mv Bioreactor Air-Lift
Chlorella Sp.
Acumulación de Lípidos
Escalado
Hidrolizados de Materiales Lignocelulósicos
topic Bioreactor Air-Lift
Chlorella Sp.
Acumulación de Lípidos
Escalado
Hidrolizados de Materiales Lignocelulósicos
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An optimal medium to culture Chlorella sp., microalgae capable of storage intracellular lipids was obtained. This culture medium consists of a saline base plus carbon-energy and nitrogen sources. Significant factors exerting influence on the culture parameters were selected. Then, by applying response surface methodology coupled to desirability function, an optimal formulation, specific for the heterotrophic growth of Chlorella sp. that allows maximizing lipid concentration was obtained. During the experimental verification, the possibility of replacing commercial glucose by hydrolysates obtained from lignocellulosic materials was evaluated. Biochemical hydrolysate of corn bran allowed obtaining important improvements in lipid concentration. Finally, the optimal formulation was evaluated in an air-lift bioreactor performing a fed-batch culture. Culturing the strain in these conditions allowed rising lipid concentrations.
Fil: Giordano, Pablo César. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Beccaria, Alejandro José. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description An optimal medium to culture Chlorella sp., microalgae capable of storage intracellular lipids was obtained. This culture medium consists of a saline base plus carbon-energy and nitrogen sources. Significant factors exerting influence on the culture parameters were selected. Then, by applying response surface methodology coupled to desirability function, an optimal formulation, specific for the heterotrophic growth of Chlorella sp. that allows maximizing lipid concentration was obtained. During the experimental verification, the possibility of replacing commercial glucose by hydrolysates obtained from lignocellulosic materials was evaluated. Biochemical hydrolysate of corn bran allowed obtaining important improvements in lipid concentration. Finally, the optimal formulation was evaluated in an air-lift bioreactor performing a fed-batch culture. Culturing the strain in these conditions allowed rising lipid concentrations.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31263
Goicoechea, Hector Casimiro; Beccaria, Alejandro José; Giordano, Pablo César; Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor; Elsevier; Bioresource Technology; 158; 2-2014; 266-277
0960-8524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31263
identifier_str_mv Goicoechea, Hector Casimiro; Beccaria, Alejandro José; Giordano, Pablo César; Rational design of a culture medium for the intensification of lipid storage in Chlorella sp. Performance evaluation in air-lift bioreactor; Elsevier; Bioresource Technology; 158; 2-2014; 266-277
0960-8524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0960852414002089
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2014.02.037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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