Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia

Autores
Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Zerba, Eduardo Nicolás; Picollo, Maria Ines
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Recently, high resistance to pyrethroid insecticides has been associated with ineffective field treatments against Triatoma infestans (Klug) in northern Argentina. Samples were collected from two areas in Argentina (Salta and La Rioja) and one in Bolivia (Yacuiba), and were subjected to toxicological and biochemical assays. All populations were resistant to deltamethrin but they showed different profiles to non-pyrethroid insecticides. Salta population showed high resistance ratios (RR) to deltamethrin and only slight differences in the susceptibility to fenitrothion and fipronil compared to the reference strain. Otherwise, La Rioja population showed a lower RR to deltamethrin and no resistance to fenitrothion or fipronil. Finally, Yacuiba population had high a RR to deltamethrin but was susceptibility to fenitrothion and fipronil. In several cases deltamethrin resistant populations had higher susceptibility to bendiocarb than the reference strain. Measured activity of P450 mono-oxygenase in individual insects (based on ethoxycoumarine-O-de-ethylase), tended to be higher in the deltamethrin resistant populations but the differences were not statistically significant. Activity of specific esterases determined by the hydrolysis of 7-coumaryl permethrate, demonstrated an increase in the percentage of insects with higher esterase activity in Salta and La Rioja populations. Unexpectedly, Yacuiba population showed lower pyrethroid esterase activity than the reference strain. The different pyrethroid resistance patterns found in T. infestans from three geographical regions within Argentina and in Bolivia, suggests that enzyme based pyrethroid resistance in this species has multiple origins. Nevertheless, as nerve insensitivity (related to the presence of the kdr gene) is also an important mechanism related to pyrethroid resistance, further studies on the kdr gene should be carried to clarify the relative contribution of each pyrethroid associated mechanism in deltamethrin resistant populations of T. infestans Keywords: Triatoma infestans, insecticide resistance, resistance ratios, mono-oxygenases, pyrethroid esterases.
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Materia
Monooxygenase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82765
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/82765
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and BoliviaSanto Orihuela, Pablo LuisVassena, Claudia VivianaZerba, Eduardo NicolásPicollo, Maria InesMonooxygenasehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Recently, high resistance to pyrethroid insecticides has been associated with ineffective field treatments against Triatoma infestans (Klug) in northern Argentina. Samples were collected from two areas in Argentina (Salta and La Rioja) and one in Bolivia (Yacuiba), and were subjected to toxicological and biochemical assays. All populations were resistant to deltamethrin but they showed different profiles to non-pyrethroid insecticides. Salta population showed high resistance ratios (RR) to deltamethrin and only slight differences in the susceptibility to fenitrothion and fipronil compared to the reference strain. Otherwise, La Rioja population showed a lower RR to deltamethrin and no resistance to fenitrothion or fipronil. Finally, Yacuiba population had high a RR to deltamethrin but was susceptibility to fenitrothion and fipronil. In several cases deltamethrin resistant populations had higher susceptibility to bendiocarb than the reference strain. Measured activity of P450 mono-oxygenase in individual insects (based on ethoxycoumarine-O-de-ethylase), tended to be higher in the deltamethrin resistant populations but the differences were not statistically significant. Activity of specific esterases determined by the hydrolysis of 7-coumaryl permethrate, demonstrated an increase in the percentage of insects with higher esterase activity in Salta and La Rioja populations. Unexpectedly, Yacuiba population showed lower pyrethroid esterase activity than the reference strain. The different pyrethroid resistance patterns found in T. infestans from three geographical regions within Argentina and in Bolivia, suggests that enzyme based pyrethroid resistance in this species has multiple origins. Nevertheless, as nerve insensitivity (related to the presence of the kdr gene) is also an important mechanism related to pyrethroid resistance, further studies on the kdr gene should be carried to clarify the relative contribution of each pyrethroid associated mechanism in deltamethrin resistant populations of T. infestans Keywords: Triatoma infestans, insecticide resistance, resistance ratios, mono-oxygenases, pyrethroid esterases.Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaEntomological Society of America2008-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82765Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Zerba, Eduardo Nicolás; Picollo, Maria Ines; Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia; Entomological Society of America; Journal of Medical Entomology; 45; 2; 3-2008; 298-3060022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/45.2.298info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/45/2/298/865900info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:02Zoai:ri.conicet.gov.ar:11336/82765instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:02.531CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
title Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
spellingShingle Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
Santo Orihuela, Pablo Luis
Monooxygenase
title_short Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
title_full Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
title_fullStr Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
title_full_unstemmed Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
title_sort Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia
dc.creator.none.fl_str_mv Santo Orihuela, Pablo Luis
Vassena, Claudia Viviana
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author Santo Orihuela, Pablo Luis
author_facet Santo Orihuela, Pablo Luis
Vassena, Claudia Viviana
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author_role author
author2 Vassena, Claudia Viviana
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author2_role author
author
author
dc.subject.none.fl_str_mv Monooxygenase
topic Monooxygenase
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Recently, high resistance to pyrethroid insecticides has been associated with ineffective field treatments against Triatoma infestans (Klug) in northern Argentina. Samples were collected from two areas in Argentina (Salta and La Rioja) and one in Bolivia (Yacuiba), and were subjected to toxicological and biochemical assays. All populations were resistant to deltamethrin but they showed different profiles to non-pyrethroid insecticides. Salta population showed high resistance ratios (RR) to deltamethrin and only slight differences in the susceptibility to fenitrothion and fipronil compared to the reference strain. Otherwise, La Rioja population showed a lower RR to deltamethrin and no resistance to fenitrothion or fipronil. Finally, Yacuiba population had high a RR to deltamethrin but was susceptibility to fenitrothion and fipronil. In several cases deltamethrin resistant populations had higher susceptibility to bendiocarb than the reference strain. Measured activity of P450 mono-oxygenase in individual insects (based on ethoxycoumarine-O-de-ethylase), tended to be higher in the deltamethrin resistant populations but the differences were not statistically significant. Activity of specific esterases determined by the hydrolysis of 7-coumaryl permethrate, demonstrated an increase in the percentage of insects with higher esterase activity in Salta and La Rioja populations. Unexpectedly, Yacuiba population showed lower pyrethroid esterase activity than the reference strain. The different pyrethroid resistance patterns found in T. infestans from three geographical regions within Argentina and in Bolivia, suggests that enzyme based pyrethroid resistance in this species has multiple origins. Nevertheless, as nerve insensitivity (related to the presence of the kdr gene) is also an important mechanism related to pyrethroid resistance, further studies on the kdr gene should be carried to clarify the relative contribution of each pyrethroid associated mechanism in deltamethrin resistant populations of T. infestans Keywords: Triatoma infestans, insecticide resistance, resistance ratios, mono-oxygenases, pyrethroid esterases.
Fil: Santo Orihuela, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
description Recently, high resistance to pyrethroid insecticides has been associated with ineffective field treatments against Triatoma infestans (Klug) in northern Argentina. Samples were collected from two areas in Argentina (Salta and La Rioja) and one in Bolivia (Yacuiba), and were subjected to toxicological and biochemical assays. All populations were resistant to deltamethrin but they showed different profiles to non-pyrethroid insecticides. Salta population showed high resistance ratios (RR) to deltamethrin and only slight differences in the susceptibility to fenitrothion and fipronil compared to the reference strain. Otherwise, La Rioja population showed a lower RR to deltamethrin and no resistance to fenitrothion or fipronil. Finally, Yacuiba population had high a RR to deltamethrin but was susceptibility to fenitrothion and fipronil. In several cases deltamethrin resistant populations had higher susceptibility to bendiocarb than the reference strain. Measured activity of P450 mono-oxygenase in individual insects (based on ethoxycoumarine-O-de-ethylase), tended to be higher in the deltamethrin resistant populations but the differences were not statistically significant. Activity of specific esterases determined by the hydrolysis of 7-coumaryl permethrate, demonstrated an increase in the percentage of insects with higher esterase activity in Salta and La Rioja populations. Unexpectedly, Yacuiba population showed lower pyrethroid esterase activity than the reference strain. The different pyrethroid resistance patterns found in T. infestans from three geographical regions within Argentina and in Bolivia, suggests that enzyme based pyrethroid resistance in this species has multiple origins. Nevertheless, as nerve insensitivity (related to the presence of the kdr gene) is also an important mechanism related to pyrethroid resistance, further studies on the kdr gene should be carried to clarify the relative contribution of each pyrethroid associated mechanism in deltamethrin resistant populations of T. infestans Keywords: Triatoma infestans, insecticide resistance, resistance ratios, mono-oxygenases, pyrethroid esterases.
publishDate 2008
dc.date.none.fl_str_mv 2008-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82765
Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Zerba, Eduardo Nicolás; Picollo, Maria Ines; Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia; Entomological Society of America; Journal of Medical Entomology; 45; 2; 3-2008; 298-306
0022-2585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82765
identifier_str_mv Santo Orihuela, Pablo Luis; Vassena, Claudia Viviana; Zerba, Eduardo Nicolás; Picollo, Maria Ines; Relative Contribution of Monooxygenase and Esterase to Pyrethroid Resistance in Triatoma infestans (Hemiptera: Reduviidae) from Argentina and Bolivia; Entomological Society of America; Journal of Medical Entomology; 45; 2; 3-2008; 298-306
0022-2585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/45.2.298
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/45/2/298/865900
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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