The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases

Autores
Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.
Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Materia
LAST UNIVERSAL COMMON ANCESTOR
FLAVIN MONOOXYGENASES
MOLECULAR EVOLUTION
BVMO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9426
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/9426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenasesMascotti, María LauraLapadula, Walter JesúsJuri Ayub, MaximilianoLAST UNIVERSAL COMMON ANCESTORFLAVIN MONOOXYGENASESMOLECULAR EVOLUTIONBVMOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaPublic Library Of Science2015-07-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9426Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-161932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132689info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0132689info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498894/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:17Zoai:ri.conicet.gov.ar:11336/9426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:17.705CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
title The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
spellingShingle The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
Mascotti, María Laura
LAST UNIVERSAL COMMON ANCESTOR
FLAVIN MONOOXYGENASES
MOLECULAR EVOLUTION
BVMO
title_short The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
title_full The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
title_fullStr The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
title_full_unstemmed The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
title_sort The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
dc.creator.none.fl_str_mv Mascotti, María Laura
Lapadula, Walter Jesús
Juri Ayub, Maximiliano
author Mascotti, María Laura
author_facet Mascotti, María Laura
Lapadula, Walter Jesús
Juri Ayub, Maximiliano
author_role author
author2 Lapadula, Walter Jesús
Juri Ayub, Maximiliano
author2_role author
author
dc.subject.none.fl_str_mv LAST UNIVERSAL COMMON ANCESTOR
FLAVIN MONOOXYGENASES
MOLECULAR EVOLUTION
BVMO
topic LAST UNIVERSAL COMMON ANCESTOR
FLAVIN MONOOXYGENASES
MOLECULAR EVOLUTION
BVMO
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.
Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
description The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9426
Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-16
1932-6203
url http://hdl.handle.net/11336/9426
identifier_str_mv Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-16
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132689
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0132689
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498894/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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