The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases
- Autores
- Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.
Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina
Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina - Materia
-
LAST UNIVERSAL COMMON ANCESTOR
FLAVIN MONOOXYGENASES
MOLECULAR EVOLUTION
BVMO - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/9426
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spelling |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenasesMascotti, María LauraLapadula, Walter JesúsJuri Ayub, MaximilianoLAST UNIVERSAL COMMON ANCESTORFLAVIN MONOOXYGENASESMOLECULAR EVOLUTIONBVMOhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes.Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaFil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; ArgentinaPublic Library Of Science2015-07-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9426Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-161932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132689info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0132689info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498894/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:19Zoai:ri.conicet.gov.ar:11336/9426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:20.041CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
title |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
spellingShingle |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases Mascotti, María Laura LAST UNIVERSAL COMMON ANCESTOR FLAVIN MONOOXYGENASES MOLECULAR EVOLUTION BVMO |
title_short |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
title_full |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
title_fullStr |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
title_full_unstemmed |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
title_sort |
The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases |
dc.creator.none.fl_str_mv |
Mascotti, María Laura Lapadula, Walter Jesús Juri Ayub, Maximiliano |
author |
Mascotti, María Laura |
author_facet |
Mascotti, María Laura Lapadula, Walter Jesús Juri Ayub, Maximiliano |
author_role |
author |
author2 |
Lapadula, Walter Jesús Juri Ayub, Maximiliano |
author2_role |
author author |
dc.subject.none.fl_str_mv |
LAST UNIVERSAL COMMON ANCESTOR FLAVIN MONOOXYGENASES MOLECULAR EVOLUTION BVMO |
topic |
LAST UNIVERSAL COMMON ANCESTOR FLAVIN MONOOXYGENASES MOLECULAR EVOLUTION BVMO |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes. Fil: Mascotti, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina Fil: Lapadula, Walter Jesús. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina Fil: Juri Ayub, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis; Argentina |
description |
The Baeyer-Villiger Monooxygenases (BVMOs) are enzymes belonging to the "Class B" of flavin monooxygenases and are capable of performing exquisite selective oxidations.These enzymes have been studied from a biotechnological perspective, but their physiologicalsubstrates and functional roles are widely unknown. Here, we investigated the origin,taxonomic distribution and evolutionary history of the BVMO genes. By using in silicoapproaches, 98 BVMO encoding genes were detected in the three domains of life: Archaea,Bacteria and Eukarya. We found evidence for the presence of these genes in Metazoa(Hydra vulgaris, Oikopleura dioica and Adineta vaga) and Haptophyta (Emiliania huxleyi) forthe first time. Furthermore, a search for other "Class B"monooxygenases (flavoproteinmonooxygenases -FMOs - and N-hydroxylating monooxygenases -NMOs) was conducted.These sequences were also found in the three domains of life. Phylogenetic analysesof all "Class B" monooxygenases revealed that NMOs and BVMOs are monophyletic,whereas FMOs form a paraphyletic group. Based on these results, we propose that BVMOgenes were already present in the last universal common ancestor (LUCA) and their currenttaxonomic distribution is the result of differential duplication and loss of paralogous genes. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/9426 Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-16 1932-6203 |
url |
http://hdl.handle.net/11336/9426 |
identifier_str_mv |
Mascotti, María Laura; Lapadula, Walter Jesús; Juri Ayub, Maximiliano; The origin and evolution of Baeyer-Villiger monooxygenases (BVMOs): an ancestral family of flavin monooxygenases; Public Library Of Science; Plos One; 10; 7; 10-7-2015; 1-16 1932-6203 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132689 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0132689 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498894/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Of Science |
publisher.none.fl_str_mv |
Public Library Of Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842268851399557120 |
score |
13.13397 |