Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina

Autores
Gonzalez Audino, Paola Andrea; Barrios, S.; Vassena, Claudia Viviana; Mougabure Cueto, Gastón Adolfo; Zerba, Eduardo Nicolás; Picollo, Maria Ines
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We studied the profile of permethrin resistance in head lice populations infesting children 6-12 years old in schools and children´s homes in & around Buenos Aires, Argentina. Five permethrin resistant populations with different levels of resistance -Hogar Loyola (HL), Republica de Turquia (RT), Hogar Mitre (HM), Guardia de Honor (GH), Ricardo Guiraldes (RG)- and one susceptible population, Bandera Argentina (BA) were collected. Their level of resistance was evaluated and results showed resistance ratios (RR) of 13 for HL, 16 for RT, 22 for HM, 61 for GH and 69 for RG. In order to elucidate the possible involvement of the cytochrome P450 monooxygenase system in conferring permethrin resistance, ethoxycoumarin-O-deethylase (ECOD) activity was measured in abdomens of individual third instar nymphs and adults using a fluorometric assay.  The ECOD activity was lower in the susceptible BA population (4.7 ng/insect) than in the resistant ones (13.7 ng/insect for RG, 12.3 ng/insect for GH, 8.6 ng/insect for RT, 8.2 ng/insect for HL). ECOD activity was significantly correlated with the level of resistance in the field populations (r= 0.97, p=0.0009) suggesting a role for cytochrome monooxygenase P450 system in permethrin resistance by P. humanus capitis.
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Barrios, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Materia
MONOOXYGENASE
RESISTANCE INSECTICIDE
PERMETHRIN
PEDICULUS HUMANUS CAPITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/82783
Acceder
id CONICETDig_582702c92928dd800749e18753647d3b
oai_identifier_str oai:ri.conicet.gov.ar:11336/82783
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from ArgentinaGonzalez Audino, Paola AndreaBarrios, S.Vassena, Claudia VivianaMougabure Cueto, Gastón AdolfoZerba, Eduardo NicolásPicollo, Maria InesMONOOXYGENASERESISTANCE INSECTICIDEPERMETHRINPEDICULUS HUMANUS CAPITIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We studied the profile of permethrin resistance in head lice populations infesting children 6-12 years old in schools and children´s homes in & around Buenos Aires, Argentina. Five permethrin resistant populations with different levels of resistance -Hogar Loyola (HL), Republica de Turquia (RT), Hogar Mitre (HM), Guardia de Honor (GH), Ricardo Guiraldes (RG)- and one susceptible population, Bandera Argentina (BA) were collected. Their level of resistance was evaluated and results showed resistance ratios (RR) of 13 for HL, 16 for RT, 22 for HM, 61 for GH and 69 for RG. In order to elucidate the possible involvement of the cytochrome P450 monooxygenase system in conferring permethrin resistance, ethoxycoumarin-O-deethylase (ECOD) activity was measured in abdomens of individual third instar nymphs and adults using a fluorometric assay.  The ECOD activity was lower in the susceptible BA population (4.7 ng/insect) than in the resistant ones (13.7 ng/insect for RG, 12.3 ng/insect for GH, 8.6 ng/insect for RT, 8.2 ng/insect for HL). ECOD activity was significantly correlated with the level of resistance in the field populations (r= 0.97, p=0.0009) suggesting a role for cytochrome monooxygenase P450 system in permethrin resistance by P. humanus capitis.Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Barrios, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaFil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; ArgentinaEntomological Society of America2005-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/82783Gonzalez Audino, Paola Andrea; Barrios, S.; Vassena, Claudia Viviana; Mougabure Cueto, Gastón Adolfo; Zerba, Eduardo Nicolás; et al.; Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina; Entomological Society of America; Journal of Medical Entomology; 42; 3; 5-2005; 342-3450022-2585CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/42.3.342info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/42/3/342/849058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:53Zoai:ri.conicet.gov.ar:11336/82783instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:53.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
title Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
spellingShingle Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
Gonzalez Audino, Paola Andrea
MONOOXYGENASE
RESISTANCE INSECTICIDE
PERMETHRIN
PEDICULUS HUMANUS CAPITIS
title_short Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
title_full Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
title_fullStr Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
title_full_unstemmed Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
title_sort Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina
dc.creator.none.fl_str_mv Gonzalez Audino, Paola Andrea
Barrios, S.
Vassena, Claudia Viviana
Mougabure Cueto, Gastón Adolfo
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author Gonzalez Audino, Paola Andrea
author_facet Gonzalez Audino, Paola Andrea
Barrios, S.
Vassena, Claudia Viviana
Mougabure Cueto, Gastón Adolfo
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author_role author
author2 Barrios, S.
Vassena, Claudia Viviana
Mougabure Cueto, Gastón Adolfo
Zerba, Eduardo Nicolás
Picollo, Maria Ines
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MONOOXYGENASE
RESISTANCE INSECTICIDE
PERMETHRIN
PEDICULUS HUMANUS CAPITIS
topic MONOOXYGENASE
RESISTANCE INSECTICIDE
PERMETHRIN
PEDICULUS HUMANUS CAPITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We studied the profile of permethrin resistance in head lice populations infesting children 6-12 years old in schools and children´s homes in & around Buenos Aires, Argentina. Five permethrin resistant populations with different levels of resistance -Hogar Loyola (HL), Republica de Turquia (RT), Hogar Mitre (HM), Guardia de Honor (GH), Ricardo Guiraldes (RG)- and one susceptible population, Bandera Argentina (BA) were collected. Their level of resistance was evaluated and results showed resistance ratios (RR) of 13 for HL, 16 for RT, 22 for HM, 61 for GH and 69 for RG. In order to elucidate the possible involvement of the cytochrome P450 monooxygenase system in conferring permethrin resistance, ethoxycoumarin-O-deethylase (ECOD) activity was measured in abdomens of individual third instar nymphs and adults using a fluorometric assay.  The ECOD activity was lower in the susceptible BA population (4.7 ng/insect) than in the resistant ones (13.7 ng/insect for RG, 12.3 ng/insect for GH, 8.6 ng/insect for RT, 8.2 ng/insect for HL). ECOD activity was significantly correlated with the level of resistance in the field populations (r= 0.97, p=0.0009) suggesting a role for cytochrome monooxygenase P450 system in permethrin resistance by P. humanus capitis.
Fil: Gonzalez Audino, Paola Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Barrios, S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Vassena, Claudia Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Mougabure Cueto, Gastón Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
Fil: Zerba, Eduardo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Picollo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Científicas y Técnicas para la Defensa. Centro de Investigación de Plagas e Insecticidas; Argentina
description We studied the profile of permethrin resistance in head lice populations infesting children 6-12 years old in schools and children´s homes in & around Buenos Aires, Argentina. Five permethrin resistant populations with different levels of resistance -Hogar Loyola (HL), Republica de Turquia (RT), Hogar Mitre (HM), Guardia de Honor (GH), Ricardo Guiraldes (RG)- and one susceptible population, Bandera Argentina (BA) were collected. Their level of resistance was evaluated and results showed resistance ratios (RR) of 13 for HL, 16 for RT, 22 for HM, 61 for GH and 69 for RG. In order to elucidate the possible involvement of the cytochrome P450 monooxygenase system in conferring permethrin resistance, ethoxycoumarin-O-deethylase (ECOD) activity was measured in abdomens of individual third instar nymphs and adults using a fluorometric assay.  The ECOD activity was lower in the susceptible BA population (4.7 ng/insect) than in the resistant ones (13.7 ng/insect for RG, 12.3 ng/insect for GH, 8.6 ng/insect for RT, 8.2 ng/insect for HL). ECOD activity was significantly correlated with the level of resistance in the field populations (r= 0.97, p=0.0009) suggesting a role for cytochrome monooxygenase P450 system in permethrin resistance by P. humanus capitis.
publishDate 2005
dc.date.none.fl_str_mv 2005-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/82783
Gonzalez Audino, Paola Andrea; Barrios, S.; Vassena, Claudia Viviana; Mougabure Cueto, Gastón Adolfo; Zerba, Eduardo Nicolás; et al.; Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina; Entomological Society of America; Journal of Medical Entomology; 42; 3; 5-2005; 342-345
0022-2585
CONICET Digital
CONICET
url http://hdl.handle.net/11336/82783
identifier_str_mv Gonzalez Audino, Paola Andrea; Barrios, S.; Vassena, Claudia Viviana; Mougabure Cueto, Gastón Adolfo; Zerba, Eduardo Nicolás; et al.; Increased Monooxygenase Activity Associated with Resistance to Permethrin in Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina; Entomological Society of America; Journal of Medical Entomology; 42; 3; 5-2005; 342-345
0022-2585
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/jmedent/42.3.342
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jme/article/42/3/342/849058
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Entomological Society of America
publisher.none.fl_str_mv Entomological Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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