Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity

Autores
Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Suárez, Viviana Beatriz
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40°C. For the phage Cb1/342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.
Fil: Trucco, Verónica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Materia
DAIRY PROCESSES
LACTIC ACID BACTERIA
LACTOBACILLUS DELBRUECKII
PHAGE RESISTANCE
TEMPERATE BACTERIOPHAGES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138791
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/138791
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversityTrucco, Verónica MilagrosReinheimer, Jorge AlbertoQuiberoni, Andrea del LujanSuárez, Viviana BeatrizDAIRY PROCESSESLACTIC ACID BACTERIALACTOBACILLUS DELBRUECKIIPHAGE RESISTANCETEMPERATE BACTERIOPHAGEShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40°C. For the phage Cb1/342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.Fil: Trucco, Verónica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaWiley Blackwell Publishing, Inc2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138791Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Suárez, Viviana Beatriz; Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 110; 4; 4-2011; 935-9421364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2011.04945.xinfo:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2011.04945.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:34Zoai:ri.conicet.gov.ar:11336/138791instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:35.182CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
title Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
spellingShingle Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
Trucco, Verónica Milagros
DAIRY PROCESSES
LACTIC ACID BACTERIA
LACTOBACILLUS DELBRUECKII
PHAGE RESISTANCE
TEMPERATE BACTERIOPHAGES
title_short Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
title_full Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
title_fullStr Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
title_full_unstemmed Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
title_sort Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity
dc.creator.none.fl_str_mv Trucco, Verónica Milagros
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
Suárez, Viviana Beatriz
author Trucco, Verónica Milagros
author_facet Trucco, Verónica Milagros
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
Suárez, Viviana Beatriz
author_role author
author2 Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
Suárez, Viviana Beatriz
author2_role author
author
author
dc.subject.none.fl_str_mv DAIRY PROCESSES
LACTIC ACID BACTERIA
LACTOBACILLUS DELBRUECKII
PHAGE RESISTANCE
TEMPERATE BACTERIOPHAGES
topic DAIRY PROCESSES
LACTIC ACID BACTERIA
LACTOBACILLUS DELBRUECKII
PHAGE RESISTANCE
TEMPERATE BACTERIOPHAGES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40°C. For the phage Cb1/342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.
Fil: Trucco, Verónica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Suárez, Viviana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
description Aims: The aim of this work was to study the adsorption step of two new temperate bacteriophages (Cb1/204 and Cb1/342) of Lactobacillus delbrueckii and to isolate phage-resistant derivatives with interesting technological properties. Methods and Results: The effect of divalent cations, pH, temperature and cell viability on adsorption step was analysed. The Ca2+ presence was necessary for the phage Cb1/342 but not for the phage Cb1/204. Both phages showed to be stable at pH values between 3 and 8. Their adsorption rates decreased considerably at pH 8 but remained high at acid pH values. The optimum temperatures for the adsorption step were between 30 and 40°C. For the phage Cb1/342, nonviable cells adsorbed a lower quantity of phage particles in comparison with the viable ones, a fact that could be linked to disorganization of phage receptor sites and/or to the physiological cellular state. The isolation of phage-resistant derivatives with good technological properties from the sensitive strains and their relationship with the cell heterogeneity of the strains were also made. Conclusions: Characterization of the adsorption step for the first temperate Lact. delbrueckii phages isolated in Argentina was made, and phage-resistant derivatives of their host strains were obtained. Significance and Impact of the Study: Some phage-resistant derivatives isolated exhibited good technological properties with the prospective to be used at industrial level.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138791
Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Suárez, Viviana Beatriz; Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 110; 4; 4-2011; 935-942
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138791
identifier_str_mv Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Suárez, Viviana Beatriz; Adsorption of temperate phages of Lactobacillus delbrueckii strains and phage resistance linked to their cell diversity; Wiley Blackwell Publishing, Inc; Journal of Applied Microbiology; 110; 4; 4-2011; 935-942
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2672.2011.04945.x
info:eu-repo/semantics/altIdentifier/url/https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2011.04945.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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