Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies

Autores
Oresti, Gerardo Martin; Reyes, Juan G.; Aveldaño, Marta Isabel
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In seminiferous tubules, Sertoli cells play a chief role in providing structural and functional support to spermatogenesis. Those germ cells that cannot be supported are led into apoptosis and rapidly eliminated by phagocytosis. At their luminal pole, Sertoli cells also support spermiation: as spermatids are released from the seminiferous epithelium, residual bodies are rapidly engulfed and degraded in Sertoli cells. In this work we isolated cell fractions enriched in pachytene spermatocytes, spermatids, and residual bodies to study their lipids and fatty acids. None of the germ cell fractions contained cholesterol esters, confirming that within seminiferous tubules these lipids are exclusive of Sertoli cell droplets. Glycerophospholipids rich in 22:5n-6 and sphingomyelin with 28:4n-6 and 32:5n-6 were more abundant in spermatocytes and especially in spermatids than in residual bodies. In contrast, the latter contained the highest proportion of 22:5-rich triglycerides of the three fractions. When Nile Red was used as a marker of oil droplets, spermatocytes and spermatids stained faintly and residual bodies fluoresced strongly and punctuated, indicating that 22:5- rich triglycerides were the main components of lipid droplets located within these structures. In vivo, recycling of residual body constituents including lipids and fatty acids may be one of the duties of Sertoli cells
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
LIPID DROPLETS
GERM CELLS
TRIGLYCERIDES
DIFFERENTIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253290
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodiesOresti, Gerardo MartinReyes, Juan G.Aveldaño, Marta IsabelLIPID DROPLETSGERM CELLSTRIGLYCERIDESDIFFERENTIATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In seminiferous tubules, Sertoli cells play a chief role in providing structural and functional support to spermatogenesis. Those germ cells that cannot be supported are led into apoptosis and rapidly eliminated by phagocytosis. At their luminal pole, Sertoli cells also support spermiation: as spermatids are released from the seminiferous epithelium, residual bodies are rapidly engulfed and degraded in Sertoli cells. In this work we isolated cell fractions enriched in pachytene spermatocytes, spermatids, and residual bodies to study their lipids and fatty acids. None of the germ cell fractions contained cholesterol esters, confirming that within seminiferous tubules these lipids are exclusive of Sertoli cell droplets. Glycerophospholipids rich in 22:5n-6 and sphingomyelin with 28:4n-6 and 32:5n-6 were more abundant in spermatocytes and especially in spermatids than in residual bodies. In contrast, the latter contained the highest proportion of 22:5-rich triglycerides of the three fractions. When Nile Red was used as a marker of oil droplets, spermatocytes and spermatids stained faintly and residual bodies fluoresced strongly and punctuated, indicating that 22:5- rich triglycerides were the main components of lipid droplets located within these structures. In vivo, recycling of residual body constituents including lipids and fatty acids may be one of the duties of Sertoli cellsFil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; ChileFil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología MolecularMar del PlataArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253290Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies; XLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 129-1290327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:03Zoai:ri.conicet.gov.ar:11336/253290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:04.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
title Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
spellingShingle Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
Oresti, Gerardo Martin
LIPID DROPLETS
GERM CELLS
TRIGLYCERIDES
DIFFERENTIATION
title_short Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
title_full Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
title_fullStr Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
title_full_unstemmed Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
title_sort Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies
dc.creator.none.fl_str_mv Oresti, Gerardo Martin
Reyes, Juan G.
Aveldaño, Marta Isabel
author Oresti, Gerardo Martin
author_facet Oresti, Gerardo Martin
Reyes, Juan G.
Aveldaño, Marta Isabel
author_role author
author2 Reyes, Juan G.
Aveldaño, Marta Isabel
author2_role author
author
dc.subject.none.fl_str_mv LIPID DROPLETS
GERM CELLS
TRIGLYCERIDES
DIFFERENTIATION
topic LIPID DROPLETS
GERM CELLS
TRIGLYCERIDES
DIFFERENTIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In seminiferous tubules, Sertoli cells play a chief role in providing structural and functional support to spermatogenesis. Those germ cells that cannot be supported are led into apoptosis and rapidly eliminated by phagocytosis. At their luminal pole, Sertoli cells also support spermiation: as spermatids are released from the seminiferous epithelium, residual bodies are rapidly engulfed and degraded in Sertoli cells. In this work we isolated cell fractions enriched in pachytene spermatocytes, spermatids, and residual bodies to study their lipids and fatty acids. None of the germ cell fractions contained cholesterol esters, confirming that within seminiferous tubules these lipids are exclusive of Sertoli cell droplets. Glycerophospholipids rich in 22:5n-6 and sphingomyelin with 28:4n-6 and 32:5n-6 were more abundant in spermatocytes and especially in spermatids than in residual bodies. In contrast, the latter contained the highest proportion of 22:5-rich triglycerides of the three fractions. When Nile Red was used as a marker of oil droplets, spermatocytes and spermatids stained faintly and residual bodies fluoresced strongly and punctuated, indicating that 22:5- rich triglycerides were the main components of lipid droplets located within these structures. In vivo, recycling of residual body constituents including lipids and fatty acids may be one of the duties of Sertoli cells
Fil: Oresti, Gerardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Reyes, Juan G.. Pontificia Universidad Católica de Valparaíso; Chile
Fil: Aveldaño, Marta Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description In seminiferous tubules, Sertoli cells play a chief role in providing structural and functional support to spermatogenesis. Those germ cells that cannot be supported are led into apoptosis and rapidly eliminated by phagocytosis. At their luminal pole, Sertoli cells also support spermiation: as spermatids are released from the seminiferous epithelium, residual bodies are rapidly engulfed and degraded in Sertoli cells. In this work we isolated cell fractions enriched in pachytene spermatocytes, spermatids, and residual bodies to study their lipids and fatty acids. None of the germ cell fractions contained cholesterol esters, confirming that within seminiferous tubules these lipids are exclusive of Sertoli cell droplets. Glycerophospholipids rich in 22:5n-6 and sphingomyelin with 28:4n-6 and 32:5n-6 were more abundant in spermatocytes and especially in spermatids than in residual bodies. In contrast, the latter contained the highest proportion of 22:5-rich triglycerides of the three fractions. When Nile Red was used as a marker of oil droplets, spermatocytes and spermatids stained faintly and residual bodies fluoresced strongly and punctuated, indicating that 22:5- rich triglycerides were the main components of lipid droplets located within these structures. In vivo, recycling of residual body constituents including lipids and fatty acids may be one of the duties of Sertoli cells
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253290
Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies; XLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 129-129
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253290
identifier_str_mv Lipid droplets in fractions enriched in spermatocytes, spermatids and residual bodies; XLIII Reunión Anual de la Sociedad Argenitna de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2007; 129-129
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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