Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering

Autores
Fernández, Juan Manuel; Molinuevo, M. Silvina; Cortizo, María Susana; Cortizo, Ana María
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.
Materia
Ciencias Químicas
bone tissue engineering
biocompatibility
poly-ε-caprolactone
polydialkyl fumarates
hydroxyapatite
osteoblasts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4757

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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/4757
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineeringFernández, Juan ManuelMolinuevo, M. SilvinaCortizo, María SusanaCortizo, Ana MaríaCiencias Químicasbone tissue engineeringbiocompatibilitypoly-ε-caprolactonepolydialkyl fumarateshydroxyapatiteosteoblastsHydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4757enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:14Zoai:digital.cic.gba.gob.ar:11746/4757Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:14.19CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
title Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
spellingShingle Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
Fernández, Juan Manuel
Ciencias Químicas
bone tissue engineering
biocompatibility
poly-ε-caprolactone
polydialkyl fumarates
hydroxyapatite
osteoblasts
title_short Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
title_full Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
title_fullStr Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
title_full_unstemmed Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
title_sort Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
dc.creator.none.fl_str_mv Fernández, Juan Manuel
Molinuevo, M. Silvina
Cortizo, María Susana
Cortizo, Ana María
author Fernández, Juan Manuel
author_facet Fernández, Juan Manuel
Molinuevo, M. Silvina
Cortizo, María Susana
Cortizo, Ana María
author_role author
author2 Molinuevo, M. Silvina
Cortizo, María Susana
Cortizo, Ana María
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Químicas
bone tissue engineering
biocompatibility
poly-ε-caprolactone
polydialkyl fumarates
hydroxyapatite
osteoblasts
topic Ciencias Químicas
bone tissue engineering
biocompatibility
poly-ε-caprolactone
polydialkyl fumarates
hydroxyapatite
osteoblasts
dc.description.none.fl_txt_mv Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.
description Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4757
url https://digital.cic.gba.gob.ar/handle/11746/4757
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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