Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering
- Autores
- Fernández, Juan Manuel; Molinuevo, M. Silvina; Cortizo, María Susana; Cortizo, Ana María
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.
- Materia
-
Ciencias Químicas
bone tissue engineering
biocompatibility
poly-ε-caprolactone
polydialkyl fumarates
hydroxyapatite
osteoblasts - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
- OAI Identificador
- oai:digital.cic.gba.gob.ar:11746/4757
Ver los metadatos del registro completo
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Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineeringFernández, Juan ManuelMolinuevo, M. SilvinaCortizo, María SusanaCortizo, Ana MaríaCiencias Químicasbone tissue engineeringbiocompatibilitypoly-ε-caprolactonepolydialkyl fumarateshydroxyapatiteosteoblastsHydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4757enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-09-29T13:40:14Zoai:digital.cic.gba.gob.ar:11746/4757Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-09-29 13:40:14.19CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse |
dc.title.none.fl_str_mv |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
title |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
spellingShingle |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering Fernández, Juan Manuel Ciencias Químicas bone tissue engineering biocompatibility poly-ε-caprolactone polydialkyl fumarates hydroxyapatite osteoblasts |
title_short |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
title_full |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
title_fullStr |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
title_full_unstemmed |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
title_sort |
Development of an osteoconductive PCL–PDIPF–hydroxyapatite composite scaffold for bone tissue engineering |
dc.creator.none.fl_str_mv |
Fernández, Juan Manuel Molinuevo, M. Silvina Cortizo, María Susana Cortizo, Ana María |
author |
Fernández, Juan Manuel |
author_facet |
Fernández, Juan Manuel Molinuevo, M. Silvina Cortizo, María Susana Cortizo, Ana María |
author_role |
author |
author2 |
Molinuevo, M. Silvina Cortizo, María Susana Cortizo, Ana María |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ciencias Químicas bone tissue engineering biocompatibility poly-ε-caprolactone polydialkyl fumarates hydroxyapatite osteoblasts |
topic |
Ciencias Químicas bone tissue engineering biocompatibility poly-ε-caprolactone polydialkyl fumarates hydroxyapatite osteoblasts |
dc.description.none.fl_txt_mv |
Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications. |
description |
Hydroxyapatite (HAP)-containing poly-ε-caprolactone (PCL)–polydiisopropyl fumarate (PDIPF) composite (Blend) was developed as an alternative for bone tissue engineering. The physicochemical, mechanical and biocompatibility properties of these composites were evaluated using two osteoblast-like cell lines (UMR106 and MC3T3E1) and compared with the blend without HAP and PCL/HAP films. The increment in the elastic modulus and the decrease in the elongationat- break of Blend–HAP suggest that the mechanical properties of the HAP scaffolds have improved significantly. The addition of HAP to both PCL and Blend significantly improves the cell biocompatibility and osteogenicity of the scaffolds. Evidence for this notion is based in several observations: (a) HAP–polymer increases proliferation of osteoblastic cells; (b) HAP included in the blend increases the ALP expression in UMR106 cells; (c) HAP–Blend increases the type-I collagen production in both cell lines, and d) higher levels of the osteogenic transcription factor Runx-2 were detected when MC3T3E1 osteoblasts were induced to differentiate and mineralize on HAP–polymer scaffolds. In conclusion, a novel biocompatible HAP–Blend composite with uniform dispersion of semi-nano HAP particles and good interphase compatibility has been prepared successfully. The development of HAP–Blend composite, with improved physical, mechanical and osteoinductive properties, may potentially be used in bone tissue-engineering applications. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://digital.cic.gba.gob.ar/handle/11746/4757 |
url |
https://digital.cic.gba.gob.ar/handle/11746/4757 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:CIC Digital (CICBA) instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires instacron:CICBA |
reponame_str |
CIC Digital (CICBA) |
collection |
CIC Digital (CICBA) |
instname_str |
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
instacron_str |
CICBA |
institution |
CICBA |
repository.name.fl_str_mv |
CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires |
repository.mail.fl_str_mv |
marisa.degiusti@sedici.unlp.edu.ar |
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