Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes
- Autores
- Zastko, Lucián; Makinistian, Leonardo; Tvarozná, Andrea; Belyaev, Igor
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects.
Fil: Zastko, Lucián. Catholic University In Ruomberok; Eslovaquia
Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Físico- Matemáticas y Naturales; Argentina
Fil: Tvarozná, Andrea. Cancer Research Institute Of Slovak Academy Of Sciences; Eslovaquia
Fil: Belyaev, Igor. Cancer Research Institute Of Slovak Academy Of Sciences; Eslovaquia - Materia
-
APOPTOSIS
INHOMOGENEOUS MAGNETIC FIELDS
REACTIVE OXYGEN SPECIES
VIABILITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/210427
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Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood LymphocytesZastko, LuciánMakinistian, LeonardoTvarozná, AndreaBelyaev, IgorAPOPTOSISINHOMOGENEOUS MAGNETIC FIELDSREACTIVE OXYGEN SPECIESVIABILITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects.Fil: Zastko, Lucián. Catholic University In Ruomberok; EslovaquiaFil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Físico- Matemáticas y Naturales; ArgentinaFil: Tvarozná, Andrea. Cancer Research Institute Of Slovak Academy Of Sciences; EslovaquiaFil: Belyaev, Igor. Cancer Research Institute Of Slovak Academy Of Sciences; EslovaquiaMolecular Diversity Preservation International2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/210427Zastko, Lucián; Makinistian, Leonardo; Tvarozná, Andrea; Belyaev, Igor; Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 22; 11-2022; 1-101422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/22/14391info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms232214391info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:17Zoai:ri.conicet.gov.ar:11336/210427instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:17.8CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
title |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
spellingShingle |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes Zastko, Lucián APOPTOSIS INHOMOGENEOUS MAGNETIC FIELDS REACTIVE OXYGEN SPECIES VIABILITY |
title_short |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
title_full |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
title_fullStr |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
title_full_unstemmed |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
title_sort |
Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes |
dc.creator.none.fl_str_mv |
Zastko, Lucián Makinistian, Leonardo Tvarozná, Andrea Belyaev, Igor |
author |
Zastko, Lucián |
author_facet |
Zastko, Lucián Makinistian, Leonardo Tvarozná, Andrea Belyaev, Igor |
author_role |
author |
author2 |
Makinistian, Leonardo Tvarozná, Andrea Belyaev, Igor |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
APOPTOSIS INHOMOGENEOUS MAGNETIC FIELDS REACTIVE OXYGEN SPECIES VIABILITY |
topic |
APOPTOSIS INHOMOGENEOUS MAGNETIC FIELDS REACTIVE OXYGEN SPECIES VIABILITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects. Fil: Zastko, Lucián. Catholic University In Ruomberok; Eslovaquia Fil: Makinistian, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Físico- Matemáticas y Naturales; Argentina Fil: Tvarozná, Andrea. Cancer Research Institute Of Slovak Academy Of Sciences; Eslovaquia Fil: Belyaev, Igor. Cancer Research Institute Of Slovak Academy Of Sciences; Eslovaquia |
description |
In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/210427 Zastko, Lucián; Makinistian, Leonardo; Tvarozná, Andrea; Belyaev, Igor; Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 22; 11-2022; 1-10 1422-0067 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/210427 |
identifier_str_mv |
Zastko, Lucián; Makinistian, Leonardo; Tvarozná, Andrea; Belyaev, Igor; Intermittent ELF-MF Induce an Amplitude-Window Effect on Umbilical Cord Blood Lymphocytes; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 23; 22; 11-2022; 1-10 1422-0067 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/22/14391 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms232214391 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613909450850304 |
score |
13.070432 |