Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection

Autores
Costa, Juan Gabriel; Duré, Andrea Belén
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.
Fil: Costa, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
Materia
ACUTE INFECTION
ANTIGEN
BIOINFORMATICS
GRA8
SERODIAGNOSIS
TOXOPLASMOSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84430
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84430
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infectionCosta, Juan GabrielDuré, Andrea BelénACUTE INFECTIONANTIGENBIOINFORMATICSGRA8SERODIAGNOSISTOXOPLASMOSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.Fil: Costa, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; ArgentinaAcademic Press Ltd - Elsevier Science Ltd2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84430Costa, Juan Gabriel; Duré, Andrea Belén; Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 100; 11-2016; 229-2360882-4010CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0882401016302042info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2016.09.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/84430instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.5CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
title Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
spellingShingle Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
Costa, Juan Gabriel
ACUTE INFECTION
ANTIGEN
BIOINFORMATICS
GRA8
SERODIAGNOSIS
TOXOPLASMOSIS
title_short Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
title_full Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
title_fullStr Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
title_full_unstemmed Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
title_sort Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection
dc.creator.none.fl_str_mv Costa, Juan Gabriel
Duré, Andrea Belén
author Costa, Juan Gabriel
author_facet Costa, Juan Gabriel
Duré, Andrea Belén
author_role author
author2 Duré, Andrea Belén
author2_role author
dc.subject.none.fl_str_mv ACUTE INFECTION
ANTIGEN
BIOINFORMATICS
GRA8
SERODIAGNOSIS
TOXOPLASMOSIS
topic ACUTE INFECTION
ANTIGEN
BIOINFORMATICS
GRA8
SERODIAGNOSIS
TOXOPLASMOSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.
Fil: Costa, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia de Santa Fe. Ministerio de Salud y Medio Ambiente - Rosario. Hospital Provincial del Centenario; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Duré, Andrea Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Ciencias Morfológicas. Laboratorio de Biología Celular y Molecular; Argentina
description In this work, two Toxoplasma gondii GRA8 protein sequences were tested by indirect ELISA and measurement of avidity to differentiate between acute and chronic toxoplasmosis infection. Using the antigen called GRA8B, 79.7% sensitivity and 84.1% specificity was achieved detecting IgG concentrations and a 71.2% sensitivity and a 68.3% specificity detecting IgA concentrations. This study is the first to report IgA detection with GRA8 by ELISA to differentiate stages of infection. Unfortunately the indirect ELISA to detect IgM was not effective in distinguishing stages. Also, this work is the first to report that the GRA8 protein can aid the differentiation between acute and chronic phase infection by measuring IgG antibody avidity, a technique in which we obtained 85.71% and 100% of sensitivity and specificity, respectively. Finally, in silico tools were used to explain the differences in our immunochemistry results.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84430
Costa, Juan Gabriel; Duré, Andrea Belén; Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 100; 11-2016; 229-236
0882-4010
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84430
identifier_str_mv Costa, Juan Gabriel; Duré, Andrea Belén; Immunochemical evaluation of two Toxoplasma gondii GRA8 sequences to detect acute toxoplasmosis infection; Academic Press Ltd - Elsevier Science Ltd; Microbial Pathogenesis; 100; 11-2016; 229-236
0882-4010
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0882401016302042
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micpath.2016.09.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
publisher.none.fl_str_mv Academic Press Ltd - Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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