In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures

Autores
Rahmoun, Massilva; Lavery, Rowena; Laurent-Chaballier, Sabine; Bellora, Nicolás; Philip, Gayle K.; Rossitto, Moïra; Symon, Aleisha; Pailhoux, Eric; Cammas, Florence; Chung, Jessica; Bagheri-Fam, Stefan; Murphy, Mark; Bardwell, Vivian; Zarkower, David; Boizet Bonhoure, Brigitte; Clair, Philippe; Harley, Vincent R.; Poulat, Francis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS). The SCS is characterized by a precise organization of binding motifs for the Sertoli cell reprogramming factors SOX9, GATA4 and DMRT1. As SOX9 biological role in mammalian gonads is to determine Sertoli cells, we correlated this genomic signature with the presence of SOX9 on chromatin in foetal testes, therefore equating this signature to a genomic bar code of the fate of foetal Sertoli cells. Starting from the hypothesis that nuclear factors that bind to genomic regions with SCS could functionally interact with SOX9, we identified TRIM28 as a new SOX9 partner in foetal testes.
Fil: Rahmoun, Massilva. Universite de Montpellier; Francia
Fil: Lavery, Rowena. Monash University; Australia
Fil: Laurent-Chaballier, Sabine. Institut de Recherche En Cancerologie de Montpellier; Francia
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: Philip, Gayle K.. Vlsci; Australia
Fil: Rossitto, Moïra. Universite de Montpellier; Francia
Fil: Symon, Aleisha. Monash University; Australia
Fil: Pailhoux, Eric. Institut National de la Recherche Agronomique; Francia
Fil: Cammas, Florence. Institut de Recherche En Cancerologie de Montpellier; Francia
Fil: Chung, Jessica. Vlsci; Australia
Fil: Bagheri-Fam, Stefan. Monash University; Australia
Fil: Murphy, Mark. University of Minnesota; Estados Unidos
Fil: Bardwell, Vivian. University of Minnesota; Estados Unidos
Fil: Zarkower, David. University of Minnesota; Estados Unidos
Fil: Boizet Bonhoure, Brigitte. Universite de Montpellier; Francia
Fil: Clair, Philippe. Universite de Montpellier; Francia
Fil: Harley, Vincent R.. Monash University; Australia
Fil: Poulat, Francis. Universite de Montpellier; Francia
Materia
Mammalian embryonic
SOX9
Transcription
Splicing
Sequence motifs
ChIP-seq
RNA-seq
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58492
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/58492
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signaturesRahmoun, MassilvaLavery, RowenaLaurent-Chaballier, SabineBellora, NicolásPhilip, Gayle K.Rossitto, MoïraSymon, AleishaPailhoux, EricCammas, FlorenceChung, JessicaBagheri-Fam, StefanMurphy, MarkBardwell, VivianZarkower, DavidBoizet Bonhoure, BrigitteClair, PhilippeHarley, Vincent R.Poulat, FrancisMammalian embryonicSOX9TranscriptionSplicingSequence motifsChIP-seqRNA-seqhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS). The SCS is characterized by a precise organization of binding motifs for the Sertoli cell reprogramming factors SOX9, GATA4 and DMRT1. As SOX9 biological role in mammalian gonads is to determine Sertoli cells, we correlated this genomic signature with the presence of SOX9 on chromatin in foetal testes, therefore equating this signature to a genomic bar code of the fate of foetal Sertoli cells. Starting from the hypothesis that nuclear factors that bind to genomic regions with SCS could functionally interact with SOX9, we identified TRIM28 as a new SOX9 partner in foetal testes.Fil: Rahmoun, Massilva. Universite de Montpellier; FranciaFil: Lavery, Rowena. Monash University; AustraliaFil: Laurent-Chaballier, Sabine. Institut de Recherche En Cancerologie de Montpellier; FranciaFil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; ArgentinaFil: Philip, Gayle K.. Vlsci; AustraliaFil: Rossitto, Moïra. Universite de Montpellier; FranciaFil: Symon, Aleisha. Monash University; AustraliaFil: Pailhoux, Eric. Institut National de la Recherche Agronomique; FranciaFil: Cammas, Florence. Institut de Recherche En Cancerologie de Montpellier; FranciaFil: Chung, Jessica. Vlsci; AustraliaFil: Bagheri-Fam, Stefan. Monash University; AustraliaFil: Murphy, Mark. University of Minnesota; Estados UnidosFil: Bardwell, Vivian. University of Minnesota; Estados UnidosFil: Zarkower, David. University of Minnesota; Estados UnidosFil: Boizet Bonhoure, Brigitte. Universite de Montpellier; FranciaFil: Clair, Philippe. Universite de Montpellier; FranciaFil: Harley, Vincent R.. Monash University; AustraliaFil: Poulat, Francis. Universite de Montpellier; FranciaOxford University Press2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58492Rahmoun, Massilva; Lavery, Rowena; Laurent-Chaballier, Sabine; Bellora, Nicolás; Philip, Gayle K.; et al.; In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures; Oxford University Press; Nucleic Acids Research; 45; 12; 7-2017; 7191-72111362-4962CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkx328info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/45/12/7191/3787816info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:21:56Zoai:ri.conicet.gov.ar:11336/58492instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:21:57.123CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
title In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
spellingShingle In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
Rahmoun, Massilva
Mammalian embryonic
SOX9
Transcription
Splicing
Sequence motifs
ChIP-seq
RNA-seq
title_short In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
title_full In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
title_fullStr In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
title_full_unstemmed In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
title_sort In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures
dc.creator.none.fl_str_mv Rahmoun, Massilva
Lavery, Rowena
Laurent-Chaballier, Sabine
Bellora, Nicolás
Philip, Gayle K.
Rossitto, Moïra
Symon, Aleisha
Pailhoux, Eric
Cammas, Florence
Chung, Jessica
Bagheri-Fam, Stefan
Murphy, Mark
Bardwell, Vivian
Zarkower, David
Boizet Bonhoure, Brigitte
Clair, Philippe
Harley, Vincent R.
Poulat, Francis
author Rahmoun, Massilva
author_facet Rahmoun, Massilva
Lavery, Rowena
Laurent-Chaballier, Sabine
Bellora, Nicolás
Philip, Gayle K.
Rossitto, Moïra
Symon, Aleisha
Pailhoux, Eric
Cammas, Florence
Chung, Jessica
Bagheri-Fam, Stefan
Murphy, Mark
Bardwell, Vivian
Zarkower, David
Boizet Bonhoure, Brigitte
Clair, Philippe
Harley, Vincent R.
Poulat, Francis
author_role author
author2 Lavery, Rowena
Laurent-Chaballier, Sabine
Bellora, Nicolás
Philip, Gayle K.
Rossitto, Moïra
Symon, Aleisha
Pailhoux, Eric
Cammas, Florence
Chung, Jessica
Bagheri-Fam, Stefan
Murphy, Mark
Bardwell, Vivian
Zarkower, David
Boizet Bonhoure, Brigitte
Clair, Philippe
Harley, Vincent R.
Poulat, Francis
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mammalian embryonic
SOX9
Transcription
Splicing
Sequence motifs
ChIP-seq
RNA-seq
topic Mammalian embryonic
SOX9
Transcription
Splicing
Sequence motifs
ChIP-seq
RNA-seq
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS). The SCS is characterized by a precise organization of binding motifs for the Sertoli cell reprogramming factors SOX9, GATA4 and DMRT1. As SOX9 biological role in mammalian gonads is to determine Sertoli cells, we correlated this genomic signature with the presence of SOX9 on chromatin in foetal testes, therefore equating this signature to a genomic bar code of the fate of foetal Sertoli cells. Starting from the hypothesis that nuclear factors that bind to genomic regions with SCS could functionally interact with SOX9, we identified TRIM28 as a new SOX9 partner in foetal testes.
Fil: Rahmoun, Massilva. Universite de Montpellier; Francia
Fil: Lavery, Rowena. Monash University; Australia
Fil: Laurent-Chaballier, Sabine. Institut de Recherche En Cancerologie de Montpellier; Francia
Fil: Bellora, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales.; Argentina
Fil: Philip, Gayle K.. Vlsci; Australia
Fil: Rossitto, Moïra. Universite de Montpellier; Francia
Fil: Symon, Aleisha. Monash University; Australia
Fil: Pailhoux, Eric. Institut National de la Recherche Agronomique; Francia
Fil: Cammas, Florence. Institut de Recherche En Cancerologie de Montpellier; Francia
Fil: Chung, Jessica. Vlsci; Australia
Fil: Bagheri-Fam, Stefan. Monash University; Australia
Fil: Murphy, Mark. University of Minnesota; Estados Unidos
Fil: Bardwell, Vivian. University of Minnesota; Estados Unidos
Fil: Zarkower, David. University of Minnesota; Estados Unidos
Fil: Boizet Bonhoure, Brigitte. Universite de Montpellier; Francia
Fil: Clair, Philippe. Universite de Montpellier; Francia
Fil: Harley, Vincent R.. Monash University; Australia
Fil: Poulat, Francis. Universite de Montpellier; Francia
description In mammalian embryonic gonads, SOX9 is required for the determination of Sertoli cells that orchestrate testis morphogenesis. To identify genetic networks directly regulated by SOX9, we combined analysis of SOX9-bound chromatin regions from murine and bovine foetal testes with sequencing of RNA samples from mouse testes lacking Sox9. We found that SOX9 controls a conserved genetic programme that involves most of the sex-determining genes. In foetal testes, SOX9 modulates both transcription and directly or indirectly sex-specific differential splicing of its target genes through binding to genomic regions with sequence motifs that are conserved among mammals and that we called 'Sertoli Cell Signature' (SCS). The SCS is characterized by a precise organization of binding motifs for the Sertoli cell reprogramming factors SOX9, GATA4 and DMRT1. As SOX9 biological role in mammalian gonads is to determine Sertoli cells, we correlated this genomic signature with the presence of SOX9 on chromatin in foetal testes, therefore equating this signature to a genomic bar code of the fate of foetal Sertoli cells. Starting from the hypothesis that nuclear factors that bind to genomic regions with SCS could functionally interact with SOX9, we identified TRIM28 as a new SOX9 partner in foetal testes.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58492
Rahmoun, Massilva; Lavery, Rowena; Laurent-Chaballier, Sabine; Bellora, Nicolás; Philip, Gayle K.; et al.; In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures; Oxford University Press; Nucleic Acids Research; 45; 12; 7-2017; 7191-7211
1362-4962
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58492
identifier_str_mv Rahmoun, Massilva; Lavery, Rowena; Laurent-Chaballier, Sabine; Bellora, Nicolás; Philip, Gayle K.; et al.; In mammalian foetal testes, SOX9 regulates expression of its target genes by binding to genomic regions with conserved signatures; Oxford University Press; Nucleic Acids Research; 45; 12; 7-2017; 7191-7211
1362-4962
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkx328
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/45/12/7191/3787816
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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