Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes

Autores
Flor, Sabrina Andrea; Estevez, Pablo Nicolás; Tripodi, Valeria Paula; Legaspi, María Julia; Mammarela, Carlos; Lucangioli, Silvia Edith
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water were necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Estevez, Pablo Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Legaspi, María Julia. Laboratorios Raffo S. A.; Argentina
Fil: Mammarela, Carlos. Laboratorios Raffo S. A.; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Doxorubicin Encapsulation
Liposomes
Sulfate
Capillary Zone Electrophoresis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16768
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/16768
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomesFlor, Sabrina AndreaEstevez, Pablo NicolásTripodi, Valeria PaulaLegaspi, María JuliaMammarela, CarlosLucangioli, Silvia EdithDoxorubicin EncapsulationLiposomesSulfateCapillary Zone Electrophoresishttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water were necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Estevez, Pablo Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; ArgentinaFil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Legaspi, María Julia. Laboratorios Raffo S. A.; ArgentinaFil: Mammarela, Carlos. Laboratorios Raffo S. A.; ArgentinaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaBentham Science Publishers2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16768Flor, Sabrina Andrea; Estevez, Pablo Nicolás; Tripodi, Valeria Paula; Legaspi, María Julia; Mammarela, Carlos; et al.; Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 4-2014; 241-2471573-41101875-6727enginfo:eu-repo/semantics/altIdentifier/doi/10.2174/15734110113099990006info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/node/110250/articleinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:14Zoai:ri.conicet.gov.ar:11336/16768instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:14.329CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
title Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
spellingShingle Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
Flor, Sabrina Andrea
Doxorubicin Encapsulation
Liposomes
Sulfate
Capillary Zone Electrophoresis
title_short Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
title_full Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
title_fullStr Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
title_full_unstemmed Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
title_sort Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Estevez, Pablo Nicolás
Tripodi, Valeria Paula
Legaspi, María Julia
Mammarela, Carlos
Lucangioli, Silvia Edith
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Estevez, Pablo Nicolás
Tripodi, Valeria Paula
Legaspi, María Julia
Mammarela, Carlos
Lucangioli, Silvia Edith
author_role author
author2 Estevez, Pablo Nicolás
Tripodi, Valeria Paula
Legaspi, María Julia
Mammarela, Carlos
Lucangioli, Silvia Edith
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Doxorubicin Encapsulation
Liposomes
Sulfate
Capillary Zone Electrophoresis
topic Doxorubicin Encapsulation
Liposomes
Sulfate
Capillary Zone Electrophoresis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water were necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.
Fil: Flor, Sabrina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Estevez, Pablo Nicolás. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Legaspi, María Julia. Laboratorios Raffo S. A.; Argentina
Fil: Mammarela, Carlos. Laboratorios Raffo S. A.; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water were necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16768
Flor, Sabrina Andrea; Estevez, Pablo Nicolás; Tripodi, Valeria Paula; Legaspi, María Julia; Mammarela, Carlos; et al.; Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 4-2014; 241-247
1573-4110
1875-6727
url http://hdl.handle.net/11336/16768
identifier_str_mv Flor, Sabrina Andrea; Estevez, Pablo Nicolás; Tripodi, Valeria Paula; Legaspi, María Julia; Mammarela, Carlos; et al.; Development and validation of capillary electrophoretic method for determination of inorganic sulfate into doxorubicin loaded liposomes; Bentham Science Publishers; Current Analytical Chemistry; 10; 2; 4-2014; 241-247
1573-4110
1875-6727
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2174/15734110113099990006
info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/node/110250/article
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers
publisher.none.fl_str_mv Bentham Science Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268844478955520
score 13.13397

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