Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts

Autores
Flor, Sabrina Andrea; Iglesias Rando, Matías Rubén; Contin, Mario Daniel; Scioscia, Silvia Liliana; Tripodi, Valeria Paula; Lucangioli, Silvia Edith
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A CD-MEKC system using sodium dodecyl sulfate and sulfated-β-cyclodextrin to achieve the simultaneous separation of Penicillin G procaine, Penicillin G benzatine and dihydrostreptomycin sulfate in pharmaceutical and veterinary dosage forms was developed. Several parameters were evaluated, including buffer type and concentration, cyclodextrin type and concentration, and organic solvent and instrumental parameters. The optimized system consisted of 50 mM SDS, 1% sulfated-ß-cyclodextrin, and 10 mM pH 9.0 borate buffer. Capillary temperature was kept at 35°C, and an electrophoretic system was operated under positive polarity at a constant voltage of 18 kV. Validation parameters such as specificity, linearity, LOD, and LOQ, precision, accuracy, and robustness were assayed. Therefore, the developed method was found to be appropriate for quality control of Penicillin G procaine, Penicillin G benzatine, and dihydrostreptomycin sulfate.
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias Rando, Matías Rubén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scioscia, Silvia Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chiral Micellar Capillary Electrophoresis
Dihydrostreptomycin Sulfate
Penicillin G Benzatine
Penicillin G Procaine
Quality Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38753
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38753
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin saltsFlor, Sabrina AndreaIglesias Rando, Matías RubénContin, Mario DanielScioscia, Silvia LilianaTripodi, Valeria PaulaLucangioli, Silvia EdithChiral Micellar Capillary ElectrophoresisDihydrostreptomycin SulfatePenicillin G BenzatinePenicillin G ProcaineQuality Controlhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3A CD-MEKC system using sodium dodecyl sulfate and sulfated-β-cyclodextrin to achieve the simultaneous separation of Penicillin G procaine, Penicillin G benzatine and dihydrostreptomycin sulfate in pharmaceutical and veterinary dosage forms was developed. Several parameters were evaluated, including buffer type and concentration, cyclodextrin type and concentration, and organic solvent and instrumental parameters. The optimized system consisted of 50 mM SDS, 1% sulfated-ß-cyclodextrin, and 10 mM pH 9.0 borate buffer. Capillary temperature was kept at 35°C, and an electrophoretic system was operated under positive polarity at a constant voltage of 18 kV. Validation parameters such as specificity, linearity, LOD, and LOQ, precision, accuracy, and robustness were assayed. Therefore, the developed method was found to be appropriate for quality control of Penicillin G procaine, Penicillin G benzatine, and dihydrostreptomycin sulfate.Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Iglesias Rando, Matías Rubén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scioscia, Silvia Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38753Flor, Sabrina Andrea; Iglesias Rando, Matías Rubén; Contin, Mario Daniel; Scioscia, Silvia Liliana; Tripodi, Valeria Paula; et al.; Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts; Taylor; Journal of Liquid Chromatography and Related Technologies; 38; 9; 5-2015; 955-9621082-6076CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2014.999198info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10826076.2014.999198info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:59Zoai:ri.conicet.gov.ar:11336/38753instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:59.636CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
title Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
spellingShingle Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
Flor, Sabrina Andrea
Chiral Micellar Capillary Electrophoresis
Dihydrostreptomycin Sulfate
Penicillin G Benzatine
Penicillin G Procaine
Quality Control
title_short Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
title_full Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
title_fullStr Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
title_full_unstemmed Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
title_sort Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Iglesias Rando, Matías Rubén
Contin, Mario Daniel
Scioscia, Silvia Liliana
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Iglesias Rando, Matías Rubén
Contin, Mario Daniel
Scioscia, Silvia Liliana
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author_role author
author2 Iglesias Rando, Matías Rubén
Contin, Mario Daniel
Scioscia, Silvia Liliana
Tripodi, Valeria Paula
Lucangioli, Silvia Edith
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Chiral Micellar Capillary Electrophoresis
Dihydrostreptomycin Sulfate
Penicillin G Benzatine
Penicillin G Procaine
Quality Control
topic Chiral Micellar Capillary Electrophoresis
Dihydrostreptomycin Sulfate
Penicillin G Benzatine
Penicillin G Procaine
Quality Control
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A CD-MEKC system using sodium dodecyl sulfate and sulfated-β-cyclodextrin to achieve the simultaneous separation of Penicillin G procaine, Penicillin G benzatine and dihydrostreptomycin sulfate in pharmaceutical and veterinary dosage forms was developed. Several parameters were evaluated, including buffer type and concentration, cyclodextrin type and concentration, and organic solvent and instrumental parameters. The optimized system consisted of 50 mM SDS, 1% sulfated-ß-cyclodextrin, and 10 mM pH 9.0 borate buffer. Capillary temperature was kept at 35°C, and an electrophoretic system was operated under positive polarity at a constant voltage of 18 kV. Validation parameters such as specificity, linearity, LOD, and LOQ, precision, accuracy, and robustness were assayed. Therefore, the developed method was found to be appropriate for quality control of Penicillin G procaine, Penicillin G benzatine, and dihydrostreptomycin sulfate.
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iglesias Rando, Matías Rubén. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Contin, Mario Daniel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scioscia, Silvia Liliana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description A CD-MEKC system using sodium dodecyl sulfate and sulfated-β-cyclodextrin to achieve the simultaneous separation of Penicillin G procaine, Penicillin G benzatine and dihydrostreptomycin sulfate in pharmaceutical and veterinary dosage forms was developed. Several parameters were evaluated, including buffer type and concentration, cyclodextrin type and concentration, and organic solvent and instrumental parameters. The optimized system consisted of 50 mM SDS, 1% sulfated-ß-cyclodextrin, and 10 mM pH 9.0 borate buffer. Capillary temperature was kept at 35°C, and an electrophoretic system was operated under positive polarity at a constant voltage of 18 kV. Validation parameters such as specificity, linearity, LOD, and LOQ, precision, accuracy, and robustness were assayed. Therefore, the developed method was found to be appropriate for quality control of Penicillin G procaine, Penicillin G benzatine, and dihydrostreptomycin sulfate.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38753
Flor, Sabrina Andrea; Iglesias Rando, Matías Rubén; Contin, Mario Daniel; Scioscia, Silvia Liliana; Tripodi, Valeria Paula; et al.; Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts; Taylor; Journal of Liquid Chromatography and Related Technologies; 38; 9; 5-2015; 955-962
1082-6076
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38753
identifier_str_mv Flor, Sabrina Andrea; Iglesias Rando, Matías Rubén; Contin, Mario Daniel; Scioscia, Silvia Liliana; Tripodi, Valeria Paula; et al.; Development and validation of a CD-MEKC system for the simultaneous determination of dihydrostreptomycin sulfate and two benzylpenicillin salts; Taylor; Journal of Liquid Chromatography and Related Technologies; 38; 9; 5-2015; 955-962
1082-6076
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/10826076.2014.999198
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10826076.2014.999198
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor
publisher.none.fl_str_mv Taylor
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269728763019264
score 13.13397

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