Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora

Autores
Izaguirre, Miriam Mercedes; Scopel, Ana Leonor; Baldwin, Ian T.; Ballare, Carlos Luis
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound- and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), α-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.
Fil: Izaguirre, Miriam Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Scopel, Ana Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Baldwin, Ian T.. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
Materia
STRESS
MANDUCA SEXTA
UVB
HERBIVORY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216614
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/216614
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longifloraIzaguirre, Miriam MercedesScopel, Ana LeonorBaldwin, Ian T.Ballare, Carlos LuisSTRESSMANDUCA SEXTAUVBHERBIVORYhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound- and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), α-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.Fil: Izaguirre, Miriam Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; AlemaniaFil: Scopel, Ana Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; AlemaniaFil: Baldwin, Ian T.. Instituto Max Planck Institut für Chemische Okologie; AlemaniaFil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; AlemaniaAmerican Society of Plant Biologist2003-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216614Izaguirre, Miriam Mercedes; Scopel, Ana Leonor; Baldwin, Ian T.; Ballare, Carlos Luis; Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora; American Society of Plant Biologist; Plant Physiology; 132; 4; 12-2003; 1755-17670032-0889CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/132/4/1755/6111711info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.103.024323info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:23Zoai:ri.conicet.gov.ar:11336/216614instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:24.18CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
title Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
spellingShingle Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
Izaguirre, Miriam Mercedes
STRESS
MANDUCA SEXTA
UVB
HERBIVORY
title_short Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
title_full Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
title_fullStr Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
title_full_unstemmed Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
title_sort Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora
dc.creator.none.fl_str_mv Izaguirre, Miriam Mercedes
Scopel, Ana Leonor
Baldwin, Ian T.
Ballare, Carlos Luis
author Izaguirre, Miriam Mercedes
author_facet Izaguirre, Miriam Mercedes
Scopel, Ana Leonor
Baldwin, Ian T.
Ballare, Carlos Luis
author_role author
author2 Scopel, Ana Leonor
Baldwin, Ian T.
Ballare, Carlos Luis
author2_role author
author
author
dc.subject.none.fl_str_mv STRESS
MANDUCA SEXTA
UVB
HERBIVORY
topic STRESS
MANDUCA SEXTA
UVB
HERBIVORY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound- and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), α-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.
Fil: Izaguirre, Miriam Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Scopel, Ana Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Baldwin, Ian T.. Instituto Max Planck Institut für Chemische Okologie; Alemania
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Instituto Max Planck Institut für Chemische Okologie; Alemania
description The effects of solar ultraviolet (UV)-B (280-315 nm) on plants have been studied intensively over the last 2 decades in connection with research on the biological impacts of stratospheric ozone depletion. However, the molecular mechanisms that mediate plant responses to solar (ambient) UV-B and their interactions with response mechanisms activated by other stressors remain for the most part unclear. Using a microarray enriched in wound- and insect-responsive sequences, we examined expression responses of 241 genes to ambient UV-B in field-grown plants of Nicotiana longiflora Cav. Approximately 20% of the sequences represented on the array showed differential expression in response to solar UV-B. The expression responses to UV-B had parallels with those elicited by simulated Manduca sexta herbivory. The most obvious similarities were: (a) down-regulation of several photosynthesis-related genes, and (b) up-regulation of genes involved in fatty acid metabolism and oxylipin biosynthesis such as HPL (hydroperoxide lyase), α-DIOX (alpha-dioxygenase), LOX (13-lipoxygenase), and AOS (allene oxide synthase). Genes encoding a WRKY transcription factor, a ferredoxin-dependent glutamate-synthase, and several other insect-responsive genes of unknown function were also similarly regulated by UV-B and insect herbivory treatments. Our results suggest that UV-B and caterpillar herbivory activate common regulatory elements and provide a platform for understanding the mechanisms of UV-B impacts on insect herbivory that have been documented in recent field studies.
publishDate 2003
dc.date.none.fl_str_mv 2003-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216614
Izaguirre, Miriam Mercedes; Scopel, Ana Leonor; Baldwin, Ian T.; Ballare, Carlos Luis; Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora; American Society of Plant Biologist; Plant Physiology; 132; 4; 12-2003; 1755-1767
0032-0889
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216614
identifier_str_mv Izaguirre, Miriam Mercedes; Scopel, Ana Leonor; Baldwin, Ian T.; Ballare, Carlos Luis; Convergent responses to stress: Solar ultraviolet-B radiation and Manduca sexta herbivory elicit overlapping transcriptional responses in field-grown plants of Nicotiana longiflora; American Society of Plant Biologist; Plant Physiology; 132; 4; 12-2003; 1755-1767
0032-0889
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/article/132/4/1755/6111711
info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.103.024323
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Plant Biologist
publisher.none.fl_str_mv American Society of Plant Biologist
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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